Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1187 and Shew_1188 are separated by 6 nucleotides Shew_1188 and Shew_1189 are separated by 82 nucleotides Shew_1189 and Shew_1190 are separated by 123 nucleotides Shew_1190 and nusB are separated by 14 nucleotides
Shew_1187: Shew_1187 - riboflavin biosynthesis protein RibD (RefSeq), at 1,379,520 to 1,380,680
_1187
Shew_1188: Shew_1188 - riboflavin synthase subunit alpha (RefSeq), at 1,380,687 to 1,381,352
_1188
Shew_1189: Shew_1189 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (RefSeq), at 1,381,435 to 1,382,538
_1189
Shew_1190: Shew_1190 - riboflavin synthase (RefSeq), at 1,382,662 to 1,383,138
_1190
Shew_1191: nusB - transcription antitermination protein NusB (RefSeq), at 1,383,153 to 1,383,557
nusB
Position (kb)
1381
1382
1383 Strain fitness (log2 ratio)
-1
0
1
2 at 1380.772 kb on - strand, within Shew_1188 at 1381.246 kb on + strand, within Shew_1188 at 1381.456 kb on - strand at 1381.456 kb on - strand at 1381.697 kb on + strand, within Shew_1189 at 1381.765 kb on + strand, within Shew_1189 at 1381.879 kb on + strand, within Shew_1189 at 1381.905 kb on - strand, within Shew_1189 at 1382.036 kb on - strand, within Shew_1189 at 1382.074 kb on + strand, within Shew_1189 at 1382.449 kb on - strand at 1382.466 kb on + strand at 1382.482 kb on - strand at 1382.483 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 1,380,772 - Shew_1188 0.13 +1.6 1,381,246 + Shew_1188 0.84 +1.4 1,381,456 - +2.1 1,381,456 - +1.4 1,381,697 + Shew_1189 0.24 +1.7 1,381,765 + Shew_1189 0.30 +1.3 1,381,879 + Shew_1189 0.40 +1.5 1,381,905 - Shew_1189 0.43 +1.9 1,382,036 - Shew_1189 0.54 +1.9 1,382,074 + Shew_1189 0.58 +1.0 1,382,449 - +1.7 1,382,466 + +1.7 1,382,482 - +1.8 1,382,483 - +1.7
Or see this region's nucleotide sequence