Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0542 and Shew_0543 are separated by 4 nucleotides Shew_0543 and tolC are separated by 67 nucleotides tolC and Shew_0545 are separated by 263 nucleotides Shew_0545 and Shew_0546 overlap by 4 nucleotides
Shew_0542: Shew_0542 - hypothetical protein (RefSeq), at 612,691 to 613,662
_0542
Shew_0543: Shew_0543 - hypothetical protein (RefSeq), at 613,667 to 614,425
_0543
Shew_0544: tolC - outer membrane channel protein (RefSeq), at 614,493 to 615,839
tolC
Shew_0545: Shew_0545 - ADP-ribose diphosphatase (RefSeq), at 616,103 to 616,711
_0545
Shew_0546: Shew_0546 - hypothetical protein (RefSeq), at 616,708 to 617,154
_0546
Position (kb) 614
615
616 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 613.564 kb on - strand, within Shew_0542 at 613.581 kb on - strand at 613.702 kb on - strand at 614.025 kb on + strand, within Shew_0543 at 614.249 kb on - strand, within Shew_0543 at 614.414 kb on - strand at 614.637 kb on - strand, within tolC at 614.676 kb on + strand, within tolC at 615.022 kb on + strand, within tolC at 615.922 kb on + strand at 615.992 kb on + strand at 616.296 kb on + strand, within Shew_0545 at 616.304 kb on - strand, within Shew_0545 at 616.347 kb on + strand, within Shew_0545 at 616.414 kb on - strand, within Shew_0545 at 616.439 kb on - strand, within Shew_0545 at 616.700 kb on - strand at 616.781 kb on - strand, within Shew_0546 at 616.795 kb on + strand, within Shew_0546
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 613,564 - Shew_0542 0.90 -1.0 613,581 - -1.0 613,702 - +0.3 614,025 + Shew_0543 0.47 -0.8 614,249 - Shew_0543 0.77 +0.2 614,414 - -1.4 614,637 - tolC Shew_0544 0.11 -5.6 614,676 + tolC Shew_0544 0.14 -5.9 615,022 + tolC Shew_0544 0.39 -2.6 615,922 + -3.8 615,992 + -0.9 616,296 + Shew_0545 0.32 -0.1 616,304 - Shew_0545 0.33 +0.6 616,347 + Shew_0545 0.40 +0.6 616,414 - Shew_0545 0.51 -0.3 616,439 - Shew_0545 0.55 -0.2 616,700 - -0.2 616,781 - Shew_0546 0.16 -0.7 616,795 + Shew_0546 0.19 +0.7
Or see this region's nucleotide sequence