Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1563 and DORF35977 are separated by 220 nucleotides DORF35977 and tRNA-Arg are separated by 114 nucleotides tRNA-Arg and tRNA-Arg are separated by 19 nucleotides tRNA-Arg and tRNA-Arg are separated by 32 nucleotides tRNA-Arg and DORF11999 are separated by 47 nucleotides DORF11999 and cysD are separated by 76 nucleotides
DVU1563: DVU1563 - sensory box histidine kinase/response regulator (TIGR), at 1,644,419 to 1,647,538
DVU1563
DORF35977: DORF35977 - no description, at 1,647,759 to 1,648,106
DORF35977
DVU_tRNA-Arg-1: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,221 to 1,648,297
tRNA-Arg
DVU_tRNA-Arg-2: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,317 to 1,648,393
tRNA-Arg
DVU_tRNA-Arg-3: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,426 to 1,648,502
tRNA-Arg
DORF11999: DORF11999 - SEED: FIG00606676: hypothetical protein, at 1,648,550 to 1,648,987
DORF11999
DVU1566: cysD - phosphoadenosine phosphosulfate reductase, putative (TIGR), at 1,649,064 to 1,649,738
cysD
Position (kb) 1648
1649 Strain fitness (log2 ratio) -2
-1
0
1 at 1647.243 kb on - strand at 1647.326 kb on - strand at 1647.326 kb on - strand at 1647.326 kb on - strand at 1647.326 kb on - strand at 1647.575 kb on - strand at 1647.909 kb on + strand, within DORF35977 at 1647.917 kb on - strand, within DORF35977 at 1647.981 kb on - strand, within DORF35977 at 1648.038 kb on - strand, within DORF35977 at 1648.258 kb on - strand, within tRNA-Arg at 1648.258 kb on - strand, within tRNA-Arg at 1648.758 kb on - strand, within DORF11999 at 1648.853 kb on - strand, within DORF11999 at 1648.853 kb on - strand, within DORF11999 at 1648.855 kb on + strand, within DORF11999 at 1648.871 kb on + strand, within DORF11999 at 1648.893 kb on + strand, within DORF11999 at 1649.111 kb on - strand at 1649.150 kb on + strand, within cysD at 1649.196 kb on - strand, within cysD at 1649.196 kb on - strand, within cysD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 1,647,243 - -0.3 1,647,326 - -2.3 1,647,326 - -0.2 1,647,326 - -0.3 1,647,326 - -0.7 1,647,575 - -0.3 1,647,909 + DORF35977 0.43 +0.0 1,647,917 - DORF35977 0.45 -0.2 1,647,981 - DORF35977 0.64 -0.4 1,648,038 - DORF35977 0.80 -0.8 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 -1.0 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 +0.1 1,648,758 - DORF11999 0.47 -0.1 1,648,853 - DORF11999 0.69 +0.1 1,648,853 - DORF11999 0.69 -1.4 1,648,855 + DORF11999 0.70 -0.1 1,648,871 + DORF11999 0.73 -0.9 1,648,893 + DORF11999 0.78 -1.0 1,649,111 - +0.4 1,649,150 + cysD DVU1566 0.13 -0.5 1,649,196 - cysD DVU1566 0.20 -0.4 1,649,196 - cysD DVU1566 0.20 +0.2
Or see this region's nucleotide sequence