Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0924 and Dshi_R0021 are separated by 240 nucleotides Dshi_R0021 and Dshi_0925 are separated by 287 nucleotides Dshi_0925 and Dshi_0926 are separated by 51 nucleotides Dshi_0926 and Dshi_0927 are separated by 23 nucleotides
Dshi_0924: Dshi_0924 - Sporulation domain protein (RefSeq), at 947,994 to 948,800
_0924
Dshi_R0021: Dshi_R0021 - tRNA-Ser (RefSeq), at 949,041 to 949,130
_R0021
Dshi_0925: Dshi_0925 - hypothetical protein (RefSeq), at 949,418 to 949,618
_0925
Dshi_0926: Dshi_0926 - Integrase catalytic region (RefSeq), at 949,670 to 950,479
_0926
Dshi_0927: Dshi_0927 - transposase IS3/IS911 family protein (RefSeq), at 950,503 to 950,730
_0927
Position (kb)
949
950 Strain fitness (log2 ratio)
-1
0
1
2 at 948.964 kb on + strand at 948.972 kb on - strand at 948.979 kb on + strand at 948.987 kb on - strand at 949.343 kb on + strand at 949.408 kb on + strand at 949.413 kb on + strand at 949.430 kb on + strand at 949.520 kb on - strand, within Dshi_0925 at 949.541 kb on - strand, within Dshi_0925 at 949.554 kb on - strand, within Dshi_0925 at 949.599 kb on + strand at 949.607 kb on - strand at 949.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 948,964 + -1.0 948,972 - -0.3 948,979 + -0.3 948,987 - +0.4 949,343 + +1.0 949,408 + -0.0 949,413 + -0.0 949,430 + -1.4 949,520 - Dshi_0925 0.51 +1.8 949,541 - Dshi_0925 0.61 -0.1 949,554 - Dshi_0925 0.68 +1.6 949,599 + +0.6 949,607 - -0.3 949,622 - -1.3
Or see this region's nucleotide sequence