Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4193 and BT4194 are separated by 269 nucleotides BT4194 and BT4195 are separated by 27 nucleotides BT4195 and BT4196 are separated by 78 nucleotides BT4196 and BT4197 are separated by 89 nucleotides
BT4193: BT4193 - dipeptidyl peptidase IV (NCBI ptt file), at 5,527,974 to 5,530,184
BT4193
BT4194: BT4194 - putative integral membrane protein (NCBI ptt file), at 5,530,454 to 5,531,401
BT4194
BT4195: BT4195 - conserved hypothetical protein (NCBI ptt file), at 5,531,429 to 5,531,641
BT4195
BT4196: BT4196 - hypothetical protein (NCBI ptt file), at 5,531,720 to 5,532,022
BT4196
BT4197: BT4197 - hypothetical protein (NCBI ptt file), at 5,532,112 to 5,532,417
BT4197
Position (kb)
5530
5531
5532 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5529.456 kb on - strand, within BT4193 at 5529.539 kb on + strand, within BT4193 at 5529.539 kb on + strand, within BT4193 at 5529.539 kb on + strand, within BT4193 at 5529.590 kb on - strand, within BT4193 at 5529.590 kb on - strand, within BT4193 at 5529.591 kb on + strand, within BT4193 at 5529.684 kb on + strand, within BT4193 at 5529.789 kb on - strand, within BT4193 at 5529.818 kb on - strand, within BT4193 at 5529.819 kb on + strand, within BT4193 at 5529.820 kb on - strand, within BT4193 at 5529.820 kb on - strand, within BT4193 at 5529.821 kb on + strand, within BT4193 at 5529.838 kb on + strand, within BT4193 at 5529.907 kb on + strand, within BT4193 at 5529.907 kb on + strand, within BT4193 at 5529.908 kb on - strand, within BT4193 at 5530.167 kb on + strand at 5530.271 kb on - strand at 5530.293 kb on - strand at 5530.301 kb on - strand at 5530.363 kb on + strand at 5530.363 kb on + strand at 5530.364 kb on - strand at 5530.490 kb on - strand at 5530.616 kb on - strand, within BT4194 at 5530.666 kb on + strand, within BT4194 at 5530.671 kb on + strand, within BT4194 at 5530.732 kb on + strand, within BT4194 at 5530.733 kb on - strand, within BT4194 at 5530.799 kb on - strand, within BT4194 at 5530.822 kb on - strand, within BT4194 at 5530.856 kb on + strand, within BT4194 at 5530.857 kb on - strand, within BT4194 at 5530.900 kb on + strand, within BT4194 at 5531.071 kb on + strand, within BT4194 at 5531.128 kb on - strand, within BT4194 at 5531.141 kb on - strand, within BT4194 at 5531.143 kb on + strand, within BT4194 at 5531.143 kb on + strand, within BT4194 at 5531.144 kb on - strand, within BT4194 at 5531.253 kb on + strand, within BT4194 at 5531.254 kb on - strand, within BT4194 at 5531.420 kb on - strand at 5531.472 kb on + strand, within BT4195 at 5531.551 kb on + strand, within BT4195 at 5531.640 kb on - strand at 5531.669 kb on + strand at 5531.748 kb on - strand at 5531.772 kb on + strand, within BT4196 at 5532.008 kb on - strand at 5532.021 kb on - strand at 5532.024 kb on - strand at 5532.062 kb on - strand at 5532.064 kb on - strand at 5532.064 kb on - strand at 5532.074 kb on + strand at 5532.075 kb on - strand at 5532.075 kb on - strand at 5532.098 kb on + strand at 5532.099 kb on - strand at 5532.101 kb on - strand at 5532.101 kb on - strand at 5532.185 kb on + strand, within BT4197 at 5532.198 kb on + strand, within BT4197 at 5532.208 kb on - strand, within BT4197 at 5532.215 kb on + strand, within BT4197 at 5532.234 kb on - strand, within BT4197 at 5532.237 kb on + strand, within BT4197 at 5532.240 kb on + strand, within BT4197 at 5532.282 kb on - strand, within BT4197 at 5532.344 kb on + strand, within BT4197 at 5532.345 kb on - strand, within BT4197 at 5532.367 kb on - strand, within BT4197 at 5532.377 kb on + strand, within BT4197 at 5532.401 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 5,529,456 - BT4193 0.67 -1.8 5,529,539 + BT4193 0.71 -1.0 5,529,539 + BT4193 0.71 -0.5 5,529,539 + BT4193 0.71 -1.0 5,529,590 - BT4193 0.73 -1.0 5,529,590 - BT4193 0.73 -1.2 5,529,591 + BT4193 0.73 +0.4 5,529,684 + BT4193 0.77 -2.9 5,529,789 - BT4193 0.82 +1.0 5,529,818 - BT4193 0.83 -2.4 5,529,819 + BT4193 0.83 -0.3 5,529,820 - BT4193 0.83 -1.4 5,529,820 - BT4193 0.83 -1.4 5,529,821 + BT4193 0.84 -1.3 5,529,838 + BT4193 0.84 -1.9 5,529,907 + BT4193 0.87 -2.2 5,529,907 + BT4193 0.87 -0.8 5,529,908 - BT4193 0.87 -1.0 5,530,167 + -0.8 5,530,271 - -0.6 5,530,293 - +0.1 5,530,301 - -3.0 5,530,363 + -1.1 5,530,363 + -1.9 5,530,364 - -2.3 5,530,490 - +0.1 5,530,616 - BT4194 0.17 -1.9 5,530,666 + BT4194 0.22 -0.6 5,530,671 + BT4194 0.23 -1.6 5,530,732 + BT4194 0.29 -0.6 5,530,733 - BT4194 0.29 +0.8 5,530,799 - BT4194 0.36 -1.6 5,530,822 - BT4194 0.39 -1.1 5,530,856 + BT4194 0.42 -2.3 5,530,857 - BT4194 0.43 +0.2 5,530,900 + BT4194 0.47 -1.6 5,531,071 + BT4194 0.65 -1.9 5,531,128 - BT4194 0.71 +0.7 5,531,141 - BT4194 0.72 -2.1 5,531,143 + BT4194 0.73 -1.0 5,531,143 + BT4194 0.73 -1.1 5,531,144 - BT4194 0.73 -1.6 5,531,253 + BT4194 0.84 +2.0 5,531,254 - BT4194 0.84 -1.6 5,531,420 - -0.1 5,531,472 + BT4195 0.20 -1.8 5,531,551 + BT4195 0.57 -0.7 5,531,640 - -0.8 5,531,669 + +0.4 5,531,748 - -1.6 5,531,772 + BT4196 0.17 -0.2 5,532,008 - -3.0 5,532,021 - +0.7 5,532,024 - -2.6 5,532,062 - -1.9 5,532,064 - -0.5 5,532,064 - -2.8 5,532,074 + +1.3 5,532,075 - -0.9 5,532,075 - -1.3 5,532,098 + +0.5 5,532,099 - -2.7 5,532,101 - -2.7 5,532,101 - +1.2 5,532,185 + BT4197 0.24 -0.3 5,532,198 + BT4197 0.28 -0.2 5,532,208 - BT4197 0.31 -0.0 5,532,215 + BT4197 0.34 -0.6 5,532,234 - BT4197 0.40 -1.3 5,532,237 + BT4197 0.41 +3.0 5,532,240 + BT4197 0.42 -0.3 5,532,282 - BT4197 0.56 -1.1 5,532,344 + BT4197 0.76 -1.6 5,532,345 - BT4197 0.76 +0.1 5,532,367 - BT4197 0.83 +0.4 5,532,377 + BT4197 0.87 -0.3 5,532,401 - -0.8
Or see this region's nucleotide sequence