Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1560 and BT1561 overlap by 8 nucleotides BT1561 and BT1562 are separated by 127 nucleotides BT1562 and BT1563 are separated by 28 nucleotides BT1563 and BT1564 are separated by 31 nucleotides
BT1560: BT1560 - nicotinate-nucleotide pyrophosphorylase (NCBI ptt file), at 1,926,988 to 1,927,836
BT1560
BT1561: BT1561 - hypothetical protein (NCBI ptt file), at 1,927,829 to 1,928,221
BT1561
BT1562: BT1562 - conserved hypothetical protein (NCBI ptt file), at 1,928,349 to 1,928,822
BT1562
BT1563: BT1563 - conserved hypothetical protein (NCBI ptt file), at 1,928,851 to 1,929,489
BT1563
BT1564: BT1564 - conserved hypothetical protein (NCBI ptt file), at 1,929,521 to 1,929,976
BT1564
Position (kb)
1928
1929 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1927.863 kb on - strand at 1927.870 kb on - strand, within BT1561 at 1928.095 kb on - strand, within BT1561 at 1928.394 kb on + strand at 1928.395 kb on - strand at 1928.397 kb on - strand, within BT1562 at 1928.399 kb on + strand, within BT1562 at 1928.400 kb on - strand, within BT1562 at 1928.400 kb on - strand, within BT1562 at 1928.400 kb on - strand, within BT1562 at 1928.406 kb on - strand, within BT1562 at 1928.408 kb on + strand, within BT1562 at 1928.651 kb on - strand, within BT1562 at 1928.651 kb on - strand, within BT1562 at 1928.692 kb on - strand, within BT1562 at 1928.692 kb on - strand, within BT1562 at 1928.699 kb on - strand, within BT1562 at 1928.702 kb on + strand, within BT1562 at 1928.749 kb on + strand, within BT1562 at 1928.820 kb on + strand at 1928.821 kb on - strand at 1928.876 kb on + strand at 1928.876 kb on + strand at 1928.982 kb on - strand, within BT1563 at 1928.996 kb on + strand, within BT1563 at 1928.997 kb on - strand, within BT1563 at 1929.029 kb on + strand, within BT1563 at 1929.141 kb on - strand, within BT1563 at 1929.145 kb on - strand, within BT1563 at 1929.295 kb on + strand, within BT1563 at 1929.325 kb on - strand, within BT1563 at 1929.325 kb on - strand, within BT1563 at 1929.325 kb on - strand, within BT1563 at 1929.327 kb on - strand, within BT1563 at 1929.327 kb on - strand, within BT1563 at 1929.350 kb on + strand, within BT1563 at 1929.400 kb on + strand, within BT1563 at 1929.456 kb on - strand at 1929.456 kb on - strand at 1929.476 kb on - strand at 1929.476 kb on - strand at 1929.477 kb on + strand at 1929.477 kb on + strand at 1929.478 kb on - strand at 1929.480 kb on + strand at 1929.480 kb on + strand at 1929.490 kb on - strand at 1929.500 kb on + strand at 1929.500 kb on + strand at 1929.503 kb on + strand at 1929.512 kb on + strand at 1929.735 kb on + strand, within BT1564 at 1929.766 kb on + strand, within BT1564
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 1,927,863 - -1.0 1,927,870 - BT1561 0.10 -1.3 1,928,095 - BT1561 0.68 -1.6 1,928,394 + -3.1 1,928,395 - +0.3 1,928,397 - BT1562 0.10 +0.8 1,928,399 + BT1562 0.11 +1.4 1,928,400 - BT1562 0.11 +2.3 1,928,400 - BT1562 0.11 -1.0 1,928,400 - BT1562 0.11 +0.7 1,928,406 - BT1562 0.12 -0.7 1,928,408 + BT1562 0.12 +1.3 1,928,651 - BT1562 0.64 +0.6 1,928,651 - BT1562 0.64 -1.8 1,928,692 - BT1562 0.72 +0.4 1,928,692 - BT1562 0.72 +0.5 1,928,699 - BT1562 0.74 -2.2 1,928,702 + BT1562 0.74 +0.8 1,928,749 + BT1562 0.84 -1.8 1,928,820 + -1.7 1,928,821 - -2.3 1,928,876 + -0.8 1,928,876 + -1.4 1,928,982 - BT1563 0.21 -1.8 1,928,996 + BT1563 0.23 -2.0 1,928,997 - BT1563 0.23 -1.0 1,929,029 + BT1563 0.28 +0.8 1,929,141 - BT1563 0.45 -1.9 1,929,145 - BT1563 0.46 -0.4 1,929,295 + BT1563 0.69 +1.1 1,929,325 - BT1563 0.74 -0.1 1,929,325 - BT1563 0.74 -2.6 1,929,325 - BT1563 0.74 +1.5 1,929,327 - BT1563 0.74 -1.6 1,929,327 - BT1563 0.74 -1.3 1,929,350 + BT1563 0.78 +0.7 1,929,400 + BT1563 0.86 -1.2 1,929,456 - -2.4 1,929,456 - +2.1 1,929,476 - -3.2 1,929,476 - -2.4 1,929,477 + -2.5 1,929,477 + -0.7 1,929,478 - -0.9 1,929,480 + -2.0 1,929,480 + -1.4 1,929,490 - +1.6 1,929,500 + +1.1 1,929,500 + +0.4 1,929,503 + -0.7 1,929,512 + -0.4 1,929,735 + BT1564 0.47 -0.2 1,929,766 + BT1564 0.54 -0.4
Or see this region's nucleotide sequence