Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1450 and BT1451 are separated by 245 nucleotides BT1451 and BT1452 are separated by 24 nucleotides BT1452 and BT1453 are separated by 240 nucleotides
BT1450: BT1450 - propionyl-CoA carboxylase beta chain (NCBI ptt file), at 1,785,763 to 1,787,307
BT1450
BT1451: BT1451 - hypothetical protein (NCBI ptt file), at 1,787,553 to 1,788,140
BT1451
BT1452: BT1452 - conserved hypothetical protein (NCBI ptt file), at 1,788,165 to 1,789,619
BT1452
BT1453: BT1453 - L-lactate permease (NCBI ptt file), at 1,789,860 to 1,791,359
BT1453
Position (kb)
1788
1789
1790 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1787.307 kb on + strand at 1787.308 kb on - strand at 1787.318 kb on + strand at 1787.398 kb on - strand at 1787.430 kb on - strand at 1787.501 kb on + strand at 1787.515 kb on + strand at 1787.565 kb on - strand at 1787.641 kb on - strand, within BT1451 at 1787.755 kb on - strand, within BT1451 at 1787.804 kb on - strand, within BT1451 at 1787.965 kb on - strand, within BT1451 at 1787.975 kb on + strand, within BT1451 at 1788.038 kb on + strand, within BT1451 at 1788.038 kb on + strand, within BT1451 at 1788.126 kb on + strand at 1788.180 kb on - strand at 1788.191 kb on + strand at 1788.192 kb on - strand at 1788.193 kb on + strand at 1788.211 kb on + strand at 1788.212 kb on - strand at 1788.265 kb on + strand at 1788.384 kb on - strand, within BT1452 at 1788.489 kb on + strand, within BT1452 at 1788.634 kb on + strand, within BT1452 at 1788.634 kb on + strand, within BT1452 at 1788.853 kb on + strand, within BT1452 at 1788.853 kb on + strand, within BT1452 at 1789.167 kb on + strand, within BT1452 at 1789.291 kb on + strand, within BT1452 at 1789.298 kb on + strand, within BT1452 at 1789.299 kb on - strand, within BT1452 at 1789.299 kb on - strand, within BT1452 at 1789.303 kb on - strand, within BT1452 at 1789.328 kb on - strand, within BT1452 at 1789.348 kb on + strand, within BT1452 at 1789.352 kb on + strand, within BT1452 at 1789.352 kb on + strand, within BT1452 at 1789.352 kb on + strand, within BT1452 at 1789.353 kb on - strand, within BT1452 at 1789.353 kb on - strand, within BT1452 at 1789.353 kb on - strand, within BT1452 at 1789.357 kb on - strand, within BT1452 at 1789.372 kb on + strand, within BT1452 at 1789.434 kb on + strand, within BT1452 at 1789.489 kb on - strand at 1789.490 kb on + strand at 1789.509 kb on - strand at 1789.520 kb on + strand at 1789.521 kb on - strand at 1789.549 kb on + strand at 1789.602 kb on - strand at 1789.643 kb on + strand at 1789.696 kb on - strand at 1789.740 kb on - strand at 1789.788 kb on - strand at 1789.820 kb on + strand at 1789.878 kb on - strand at 1789.904 kb on - strand at 1789.904 kb on - strand at 1789.947 kb on - strand at 1789.961 kb on - strand at 1789.961 kb on - strand at 1790.023 kb on + strand, within BT1453 at 1790.024 kb on - strand, within BT1453 at 1790.026 kb on - strand, within BT1453 at 1790.026 kb on - strand, within BT1453 at 1790.056 kb on + strand, within BT1453 at 1790.056 kb on + strand, within BT1453 at 1790.057 kb on - strand, within BT1453 at 1790.120 kb on + strand, within BT1453 at 1790.159 kb on + strand, within BT1453 at 1790.235 kb on - strand, within BT1453 at 1790.275 kb on + strand at 1790.390 kb on + strand, within BT1453 at 1790.549 kb on + strand, within BT1453 at 1790.550 kb on - strand, within BT1453 at 1790.591 kb on + strand, within BT1453 at 1790.592 kb on - strand, within BT1453
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 1,787,307 + -2.3 1,787,308 - -0.9 1,787,318 + -1.7 1,787,398 - -0.1 1,787,430 - -1.3 1,787,501 + +0.5 1,787,515 + -1.0 1,787,565 - -1.1 1,787,641 - BT1451 0.15 +0.5 1,787,755 - BT1451 0.34 +3.8 1,787,804 - BT1451 0.43 -1.4 1,787,965 - BT1451 0.70 -1.4 1,787,975 + BT1451 0.72 -1.1 1,788,038 + BT1451 0.82 +0.2 1,788,038 + BT1451 0.82 -2.5 1,788,126 + -0.7 1,788,180 - +0.3 1,788,191 + -0.4 1,788,192 - +0.5 1,788,193 + +1.5 1,788,211 + -1.1 1,788,212 - +0.0 1,788,265 + +0.2 1,788,384 - BT1452 0.15 +1.9 1,788,489 + BT1452 0.22 -3.1 1,788,634 + BT1452 0.32 +2.9 1,788,634 + BT1452 0.32 -3.3 1,788,853 + BT1452 0.47 -1.0 1,788,853 + BT1452 0.47 +1.8 1,789,167 + BT1452 0.69 -1.4 1,789,291 + BT1452 0.77 +0.9 1,789,298 + BT1452 0.78 +0.8 1,789,299 - BT1452 0.78 -0.4 1,789,299 - BT1452 0.78 +0.7 1,789,303 - BT1452 0.78 +1.2 1,789,328 - BT1452 0.80 +1.5 1,789,348 + BT1452 0.81 -0.3 1,789,352 + BT1452 0.82 +1.2 1,789,352 + BT1452 0.82 -0.5 1,789,352 + BT1452 0.82 +0.7 1,789,353 - BT1452 0.82 -0.4 1,789,353 - BT1452 0.82 -0.7 1,789,353 - BT1452 0.82 -2.1 1,789,357 - BT1452 0.82 -0.3 1,789,372 + BT1452 0.83 -2.5 1,789,434 + BT1452 0.87 +0.5 1,789,489 - +1.2 1,789,490 + +0.6 1,789,509 - -1.8 1,789,520 + -0.1 1,789,521 - -2.4 1,789,549 + -2.3 1,789,602 - -0.4 1,789,643 + +0.9 1,789,696 - +0.2 1,789,740 - -2.9 1,789,788 - +0.4 1,789,820 + +0.8 1,789,878 - +1.2 1,789,904 - +1.5 1,789,904 - +0.9 1,789,947 - -2.8 1,789,961 - +0.2 1,789,961 - -2.4 1,790,023 + BT1453 0.11 -0.9 1,790,024 - BT1453 0.11 -2.3 1,790,026 - BT1453 0.11 -1.2 1,790,026 - BT1453 0.11 -2.1 1,790,056 + BT1453 0.13 -2.0 1,790,056 + BT1453 0.13 +2.2 1,790,057 - BT1453 0.13 +0.1 1,790,120 + BT1453 0.17 -1.6 1,790,159 + BT1453 0.20 -2.3 1,790,235 - BT1453 0.25 -0.1 1,790,275 + +1.2 1,790,390 + BT1453 0.35 +1.0 1,790,549 + BT1453 0.46 -1.7 1,790,550 - BT1453 0.46 +2.5 1,790,591 + BT1453 0.49 -0.8 1,790,592 - BT1453 0.49 -0.3
Or see this region's nucleotide sequence