Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1051 and BT1052 are separated by 59 nucleotides BT1052 and BT1053 are separated by 163 nucleotides BT1053 and BT1054 are separated by 19 nucleotides
BT1051: BT1051 - beta-hexosaminidase precursor (NCBI ptt file), at 1,332,865 to 1,335,186
BT1051
BT1052: BT1052 - putative anti-sigma factor (NCBI ptt file), at 1,335,246 to 1,336,238
BT1052
BT1053: BT1053 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 1,336,402 to 1,337,010
BT1053
BT1054: BT1054 - ATP-dependent helicase (NCBI ptt file), at 1,337,030 to 1,340,203
BT1054
Position (kb)
1335
1336
1337 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1334.301 kb on - strand, within BT1051 at 1334.301 kb on - strand, within BT1051 at 1334.323 kb on + strand, within BT1051 at 1334.324 kb on - strand, within BT1051 at 1334.463 kb on + strand, within BT1051 at 1334.468 kb on - strand, within BT1051 at 1334.504 kb on + strand, within BT1051 at 1334.540 kb on + strand, within BT1051 at 1334.566 kb on + strand, within BT1051 at 1334.592 kb on + strand, within BT1051 at 1334.608 kb on - strand, within BT1051 at 1334.610 kb on - strand, within BT1051 at 1334.907 kb on - strand, within BT1051 at 1334.914 kb on + strand, within BT1051 at 1334.914 kb on + strand, within BT1051 at 1334.925 kb on - strand, within BT1051 at 1334.989 kb on - strand at 1335.034 kb on - strand at 1335.085 kb on + strand at 1335.112 kb on + strand at 1335.115 kb on - strand at 1335.119 kb on + strand at 1335.164 kb on + strand at 1335.165 kb on - strand at 1335.217 kb on - strand at 1335.321 kb on + strand at 1335.505 kb on + strand, within BT1052 at 1335.537 kb on - strand, within BT1052 at 1335.539 kb on + strand, within BT1052 at 1335.803 kb on - strand, within BT1052 at 1335.807 kb on - strand, within BT1052 at 1336.001 kb on + strand, within BT1052 at 1336.095 kb on - strand, within BT1052 at 1336.117 kb on + strand, within BT1052 at 1336.117 kb on + strand, within BT1052 at 1336.120 kb on + strand, within BT1052 at 1336.134 kb on + strand, within BT1052 at 1336.134 kb on + strand, within BT1052 at 1336.213 kb on + strand at 1336.311 kb on - strand at 1336.371 kb on + strand at 1336.418 kb on + strand at 1336.420 kb on + strand at 1336.428 kb on + strand at 1336.571 kb on + strand, within BT1053 at 1336.614 kb on + strand, within BT1053 at 1336.971 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 1,334,301 - BT1051 0.62 -1.5 1,334,301 - BT1051 0.62 +1.2 1,334,323 + BT1051 0.63 -1.2 1,334,324 - BT1051 0.63 +0.7 1,334,463 + BT1051 0.69 -0.7 1,334,468 - BT1051 0.69 -1.3 1,334,504 + BT1051 0.71 -2.5 1,334,540 + BT1051 0.72 -1.2 1,334,566 + BT1051 0.73 -3.3 1,334,592 + BT1051 0.74 +0.4 1,334,608 - BT1051 0.75 -2.2 1,334,610 - BT1051 0.75 -1.5 1,334,907 - BT1051 0.88 -1.7 1,334,914 + BT1051 0.88 +0.2 1,334,914 + BT1051 0.88 -0.9 1,334,925 - BT1051 0.89 -2.9 1,334,989 - -3.8 1,335,034 - -1.5 1,335,085 + -0.1 1,335,112 + +1.1 1,335,115 - +0.4 1,335,119 + -3.0 1,335,164 + +0.6 1,335,165 - -1.5 1,335,217 - +0.6 1,335,321 + +0.2 1,335,505 + BT1052 0.26 -2.3 1,335,537 - BT1052 0.29 -0.9 1,335,539 + BT1052 0.30 +0.3 1,335,803 - BT1052 0.56 -2.9 1,335,807 - BT1052 0.56 -2.5 1,336,001 + BT1052 0.76 -3.4 1,336,095 - BT1052 0.85 -1.5 1,336,117 + BT1052 0.88 -0.7 1,336,117 + BT1052 0.88 +1.1 1,336,120 + BT1052 0.88 +2.1 1,336,134 + BT1052 0.89 -1.6 1,336,134 + BT1052 0.89 -1.1 1,336,213 + +0.1 1,336,311 - +0.7 1,336,371 + -2.1 1,336,418 + -2.8 1,336,420 + -2.0 1,336,428 + -1.5 1,336,571 + BT1053 0.28 +0.6 1,336,614 + BT1053 0.35 -1.1 1,336,971 + +1.5
Or see this region's nucleotide sequence