Experiment: BHIS with Fusidic 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0457 and BT0458 are separated by 77 nucleotides BT0458 and BT0459 are separated by 38 nucleotides
BT0457: BT0457 - sialic acid-specific 9-O-acetylesterase (NCBI ptt file), at 561,110 to 563,188
BT0457
BT0458: BT0458 - beta-mannosidase precursor (NCBI ptt file), at 563,266 to 565,860
BT0458
BT0459: BT0459 - beta-hexosaminidase precursor (NCBI ptt file), at 565,899 to 568,223
BT0459
Position (kb) 563
564
565
566 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 562.497 kb on - strand, within BT0457 at 562.512 kb on + strand, within BT0457 at 562.677 kb on - strand, within BT0457 at 562.681 kb on - strand, within BT0457 at 562.810 kb on + strand, within BT0457 at 562.810 kb on + strand, within BT0457 at 562.810 kb on + strand, within BT0457 at 562.816 kb on + strand, within BT0457 at 562.816 kb on + strand, within BT0457 at 562.935 kb on + strand, within BT0457 at 563.063 kb on + strand at 563.064 kb on - strand at 563.266 kb on - strand at 563.325 kb on + strand at 563.329 kb on - strand at 563.335 kb on + strand at 563.347 kb on - strand at 563.370 kb on - strand at 563.536 kb on + strand, within BT0458 at 563.539 kb on - strand, within BT0458 at 563.539 kb on - strand, within BT0458 at 563.550 kb on + strand, within BT0458 at 563.551 kb on - strand, within BT0458 at 563.615 kb on - strand, within BT0458 at 563.677 kb on - strand, within BT0458 at 563.721 kb on - strand, within BT0458 at 563.848 kb on - strand, within BT0458 at 563.848 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.925 kb on - strand, within BT0458 at 563.928 kb on + strand, within BT0458 at 564.051 kb on - strand, within BT0458 at 564.066 kb on + strand, within BT0458 at 564.110 kb on + strand, within BT0458 at 564.350 kb on - strand, within BT0458 at 564.350 kb on - strand, within BT0458 at 564.395 kb on - strand, within BT0458 at 564.430 kb on - strand, within BT0458 at 564.445 kb on - strand, within BT0458 at 564.506 kb on + strand, within BT0458 at 564.507 kb on - strand, within BT0458 at 565.056 kb on + strand, within BT0458 at 565.060 kb on + strand, within BT0458 at 565.078 kb on - strand, within BT0458 at 565.182 kb on + strand, within BT0458 at 565.365 kb on + strand, within BT0458 at 565.439 kb on + strand, within BT0458 at 565.440 kb on - strand, within BT0458 at 565.527 kb on + strand, within BT0458 at 565.760 kb on + strand at 565.773 kb on + strand at 565.782 kb on - strand at 565.821 kb on - strand at 565.840 kb on - strand at 565.869 kb on + strand at 566.094 kb on - strand at 566.115 kb on + strand at 566.116 kb on - strand at 566.203 kb on + strand, within BT0459 at 566.220 kb on + strand, within BT0459 at 566.221 kb on - strand, within BT0459 at 566.284 kb on - strand, within BT0459 at 566.441 kb on + strand, within BT0459 at 566.442 kb on - strand, within BT0459 at 566.546 kb on - strand, within BT0459 at 566.580 kb on - strand, within BT0459 at 566.580 kb on - strand, within BT0459 at 566.619 kb on + strand, within BT0459 at 566.620 kb on - strand, within BT0459 at 566.647 kb on + strand, within BT0459 at 566.668 kb on - strand, within BT0459 at 566.669 kb on + strand, within BT0459 at 566.670 kb on - strand, within BT0459 at 566.794 kb on + strand, within BT0459 at 566.794 kb on + strand, within BT0459 at 566.795 kb on - strand, within BT0459 at 566.839 kb on + strand, within BT0459 at 566.839 kb on + strand, within BT0459 at 566.840 kb on - strand, within BT0459
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Fusidic 0.002 mg/ml remove 562,497 - BT0457 0.67 +0.3 562,512 + BT0457 0.67 +0.0 562,677 - BT0457 0.75 +0.7 562,681 - BT0457 0.76 +2.3 562,810 + BT0457 0.82 -2.3 562,810 + BT0457 0.82 -2.4 562,810 + BT0457 0.82 +1.1 562,816 + BT0457 0.82 -1.6 562,816 + BT0457 0.82 -3.0 562,935 + BT0457 0.88 -1.9 563,063 + -1.2 563,064 - +0.9 563,266 - -2.4 563,325 + -0.9 563,329 - -1.7 563,335 + -1.1 563,347 - -2.7 563,370 - +1.1 563,536 + BT0458 0.10 +1.6 563,539 - BT0458 0.11 -2.6 563,539 - BT0458 0.11 -1.7 563,550 + BT0458 0.11 -1.3 563,551 - BT0458 0.11 -3.7 563,615 - BT0458 0.13 -1.7 563,677 - BT0458 0.16 +0.5 563,721 - BT0458 0.18 -0.6 563,848 - BT0458 0.22 -1.7 563,848 - BT0458 0.22 -2.3 563,925 - BT0458 0.25 -2.2 563,925 - BT0458 0.25 -1.4 563,925 - BT0458 0.25 +0.1 563,925 - BT0458 0.25 -1.7 563,928 + BT0458 0.26 -0.1 564,051 - BT0458 0.30 -1.2 564,066 + BT0458 0.31 +0.9 564,110 + BT0458 0.33 +0.5 564,350 - BT0458 0.42 -0.6 564,350 - BT0458 0.42 -3.3 564,395 - BT0458 0.44 +1.1 564,430 - BT0458 0.45 +0.1 564,445 - BT0458 0.45 -2.2 564,506 + BT0458 0.48 -0.2 564,507 - BT0458 0.48 -1.2 565,056 + BT0458 0.69 -2.8 565,060 + BT0458 0.69 -1.7 565,078 - BT0458 0.70 -2.4 565,182 + BT0458 0.74 -1.2 565,365 + BT0458 0.81 -2.9 565,439 + BT0458 0.84 +0.7 565,440 - BT0458 0.84 +0.3 565,527 + BT0458 0.87 +0.6 565,760 + -0.2 565,773 + -1.2 565,782 - -3.9 565,821 - -1.0 565,840 - -0.3 565,869 + -0.6 566,094 - -1.2 566,115 + +0.4 566,116 - -0.4 566,203 + BT0459 0.13 +1.1 566,220 + BT0459 0.14 -1.4 566,221 - BT0459 0.14 +1.1 566,284 - BT0459 0.17 -1.2 566,441 + BT0459 0.23 -0.4 566,442 - BT0459 0.23 +2.4 566,546 - BT0459 0.28 -1.4 566,580 - BT0459 0.29 +2.1 566,580 - BT0459 0.29 -1.8 566,619 + BT0459 0.31 -1.6 566,620 - BT0459 0.31 -1.5 566,647 + BT0459 0.32 -1.4 566,668 - BT0459 0.33 +1.1 566,669 + BT0459 0.33 -1.4 566,670 - BT0459 0.33 +0.4 566,794 + BT0459 0.38 +0.3 566,794 + BT0459 0.38 -2.2 566,795 - BT0459 0.39 +1.0 566,839 + BT0459 0.40 -3.1 566,839 + BT0459 0.40 -1.2 566,840 - BT0459 0.40 -1.9
Or see this region's nucleotide sequence