Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0603 and DVU0604 overlap by 4 nucleotides DVU0604 and DVU0605 overlap by 18 nucleotides DVU0605 and DVU0606 are separated by 272 nucleotides DVU0606 and ahcY are separated by 25 nucleotides
DVU0603: DVU0603 - hypothetical protein (TIGR), at 673,432 to 673,698
DVU0603
DVU0604: DVU0604 - hypothetical protein (TIGR), at 673,695 to 673,826
DVU0604
DVU0605: DVU0605 - hypothetical protein (TIGR), at 673,809 to 673,937
DVU0605
DVU0606: DVU0606 - transcriptional regulator, ArsR family/methyltransferase, UbiE/COQ5 family (TIGR), at 674,210 to 675,133
DVU0606
DVU0607: ahcY - adenosylhomocysteinase (TIGR), at 675,159 to 676,598
ahcY
Position (kb)
674
675
676 Strain fitness (log2 ratio)
-2
-1
0
1 at 673.491 kb on + strand, within DVU0603 at 673.509 kb on - strand, within DVU0603 at 673.613 kb on - strand, within DVU0603 at 673.994 kb on - strand at 674.025 kb on - strand at 674.042 kb on - strand at 674.121 kb on - strand at 674.291 kb on - strand at 674.407 kb on + strand, within DVU0606 at 674.437 kb on - strand, within DVU0606 at 674.454 kb on + strand, within DVU0606 at 674.497 kb on + strand, within DVU0606 at 674.532 kb on + strand, within DVU0606 at 674.579 kb on + strand, within DVU0606 at 674.602 kb on - strand, within DVU0606 at 674.622 kb on - strand, within DVU0606 at 674.644 kb on + strand, within DVU0606 at 674.715 kb on + strand, within DVU0606 at 674.787 kb on + strand, within DVU0606 at 674.872 kb on - strand, within DVU0606 at 674.938 kb on - strand, within DVU0606 at 675.056 kb on - strand at 675.096 kb on - strand at 675.098 kb on - strand at 675.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 673,491 + DVU0603 0.22 -0.4 673,509 - DVU0603 0.29 +0.9 673,613 - DVU0603 0.68 -0.4 673,994 - -1.0 674,025 - -0.9 674,042 - +0.3 674,121 - -0.1 674,291 - +0.3 674,407 + DVU0606 0.21 -0.5 674,437 - DVU0606 0.25 -0.7 674,454 + DVU0606 0.26 +0.0 674,497 + DVU0606 0.31 -1.0 674,532 + DVU0606 0.35 -0.3 674,579 + DVU0606 0.40 -1.7 674,602 - DVU0606 0.42 -0.6 674,622 - DVU0606 0.45 -0.8 674,644 + DVU0606 0.47 -0.5 674,715 + DVU0606 0.55 -0.8 674,787 + DVU0606 0.62 -1.9 674,872 - DVU0606 0.72 -0.3 674,938 - DVU0606 0.79 -0.2 675,056 - +1.0 675,096 - -2.0 675,098 - -0.3 675,145 + +0.3
Or see this region's nucleotide sequence