Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0483 and DVU0484 are separated by 202 nucleotides DVU0484 and DVU0485 overlap by 4 nucleotides DVU0485 and DVU0486 are separated by 43 nucleotides DVU0486 and purE are separated by 299 nucleotides
DVU0483: DVU0483 - DNA mismatch repair protein MutL, putative (TIGR), at 549,943 to 552,177
DVU0483
DVU0484: DVU0484 - ABC transporter, ATP-binding protein (TIGR), at 552,380 to 553,174
DVU0484
DVU0485: DVU0485 - membrane protein, putative (TIGR), at 553,171 to 553,956
DVU0485
DVU0486: DVU0486 - hypothetical protein (TIGR), at 554,000 to 554,329
DVU0486
DVU0487: purE - phosphoribosylaminoimidazole carboxylase, catalytic subunit (TIGR), at 554,629 to 555,129
purE
Position (kb) 553
554 Strain fitness (log2 ratio) -2
-1
0
1
2 at 552.194 kb on - strand at 552.210 kb on - strand at 552.217 kb on - strand at 552.250 kb on + strand at 552.333 kb on + strand at 552.341 kb on - strand at 552.341 kb on - strand at 552.341 kb on - strand at 552.341 kb on - strand at 552.360 kb on + strand at 552.360 kb on + strand at 552.374 kb on + strand at 552.374 kb on - strand at 552.460 kb on - strand, within DVU0484 at 552.540 kb on + strand, within DVU0484 at 552.622 kb on + strand, within DVU0484 at 552.706 kb on + strand, within DVU0484 at 552.778 kb on - strand, within DVU0484 at 552.804 kb on + strand, within DVU0484 at 552.812 kb on - strand, within DVU0484 at 552.826 kb on + strand, within DVU0484 at 552.829 kb on - strand, within DVU0484 at 553.016 kb on + strand, within DVU0484 at 553.144 kb on + strand at 553.275 kb on - strand, within DVU0485 at 553.305 kb on - strand, within DVU0485 at 553.389 kb on + strand, within DVU0485 at 553.390 kb on + strand, within DVU0485 at 553.398 kb on - strand, within DVU0485 at 553.398 kb on - strand, within DVU0485 at 553.427 kb on + strand, within DVU0485 at 553.442 kb on - strand, within DVU0485 at 553.467 kb on + strand, within DVU0485 at 553.482 kb on - strand, within DVU0485 at 553.554 kb on + strand, within DVU0485 at 553.554 kb on - strand, within DVU0485 at 553.620 kb on - strand, within DVU0485 at 553.631 kb on - strand, within DVU0485 at 553.633 kb on - strand, within DVU0485 at 553.662 kb on - strand, within DVU0485 at 553.679 kb on - strand, within DVU0485 at 553.800 kb on + strand, within DVU0485 at 553.809 kb on - strand, within DVU0485 at 553.902 kb on - strand at 553.902 kb on - strand at 553.957 kb on + strand at 553.970 kb on - strand at 554.005 kb on - strand at 554.089 kb on + strand, within DVU0486 at 554.149 kb on - strand, within DVU0486 at 554.162 kb on + strand, within DVU0486 at 554.250 kb on + strand, within DVU0486 at 554.268 kb on - strand, within DVU0486 at 554.331 kb on - strand at 554.408 kb on + strand at 554.423 kb on + strand at 554.457 kb on - strand at 554.514 kb on + strand at 554.631 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 552,194 - -1.3 552,210 - -1.8 552,217 - +0.1 552,250 + +0.4 552,333 + -0.2 552,341 - -0.1 552,341 - -0.0 552,341 - +0.6 552,341 - +1.6 552,360 + -0.2 552,360 + +0.2 552,374 + +0.8 552,374 - -0.0 552,460 - DVU0484 0.10 +0.1 552,540 + DVU0484 0.20 -0.3 552,622 + DVU0484 0.30 +0.7 552,706 + DVU0484 0.41 -0.1 552,778 - DVU0484 0.50 -0.3 552,804 + DVU0484 0.53 -0.6 552,812 - DVU0484 0.54 -0.2 552,826 + DVU0484 0.56 -2.0 552,829 - DVU0484 0.56 +0.6 553,016 + DVU0484 0.80 -0.8 553,144 + -0.3 553,275 - DVU0485 0.13 +0.5 553,305 - DVU0485 0.17 +0.5 553,389 + DVU0485 0.28 +0.1 553,390 + DVU0485 0.28 +0.1 553,398 - DVU0485 0.29 +0.2 553,398 - DVU0485 0.29 +0.2 553,427 + DVU0485 0.33 -0.2 553,442 - DVU0485 0.34 -0.8 553,467 + DVU0485 0.38 -0.4 553,482 - DVU0485 0.40 +0.0 553,554 + DVU0485 0.49 +2.0 553,554 - DVU0485 0.49 +0.5 553,620 - DVU0485 0.57 +0.6 553,631 - DVU0485 0.59 +0.3 553,633 - DVU0485 0.59 -0.1 553,662 - DVU0485 0.62 +0.0 553,679 - DVU0485 0.65 +0.2 553,800 + DVU0485 0.80 -1.4 553,809 - DVU0485 0.81 +0.2 553,902 - -1.3 553,902 - -0.2 553,957 + -0.8 553,970 - -0.3 554,005 - -1.0 554,089 + DVU0486 0.27 +0.2 554,149 - DVU0486 0.45 -1.0 554,162 + DVU0486 0.49 -0.1 554,250 + DVU0486 0.76 +0.4 554,268 - DVU0486 0.81 -0.1 554,331 - +0.1 554,408 + -1.5 554,423 + -2.1 554,457 - -2.3 554,514 + -0.1 554,631 + -1.2
Or see this region's nucleotide sequence