Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpD and trpC overlap by 8 nucleotides trpC and trpF-1 are separated by 47 nucleotides trpF-1 and trpB-2 overlap by 4 nucleotides
DVU0467: trpD - anthranilate phosphoribosyltransferase (TIGR), at 535,058 to 536,056
trpD
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
DVU0469: trpF-1 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 536,870 to 537,724
trpF-1
DVU0470: trpB-2 - tryptophan synthase, beta subunit (TIGR), at 537,721 to 538,908
trpB-2
Position (kb) 536
537
538 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 535.871 kb on - strand, within trpD at 535.897 kb on + strand, within trpD at 535.905 kb on - strand, within trpD at 535.920 kb on - strand, within trpD at 535.938 kb on + strand, within trpD at 535.942 kb on - strand, within trpD at 535.942 kb on - strand, within trpD at 535.955 kb on + strand, within trpD at 535.955 kb on + strand, within trpD at 535.963 kb on - strand at 535.983 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 536.095 kb on - strand at 536.158 kb on + strand, within trpC at 536.164 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.166 kb on - strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.179 kb on + strand, within trpC at 536.301 kb on - strand, within trpC at 536.303 kb on + strand, within trpC at 536.311 kb on - strand, within trpC at 536.318 kb on + strand, within trpC at 536.318 kb on + strand, within trpC at 536.369 kb on - strand, within trpC at 536.425 kb on + strand, within trpC at 536.461 kb on + strand, within trpC at 536.467 kb on + strand, within trpC at 536.506 kb on - strand, within trpC at 536.511 kb on + strand, within trpC at 536.519 kb on - strand, within trpC at 536.519 kb on - strand, within trpC at 536.547 kb on - strand, within trpC at 536.566 kb on + strand, within trpC at 536.611 kb on + strand, within trpC at 536.619 kb on - strand, within trpC at 536.669 kb on + strand, within trpC at 536.750 kb on - strand at 536.757 kb on + strand at 536.757 kb on + strand at 536.765 kb on - strand at 536.799 kb on + strand at 536.807 kb on - strand at 536.860 kb on + strand at 536.979 kb on + strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.135 kb on + strand, within trpF-1 at 537.206 kb on - strand, within trpF-1 at 537.288 kb on + strand, within trpF-1 at 537.389 kb on - strand, within trpF-1 at 537.476 kb on + strand, within trpF-1 at 537.479 kb on - strand, within trpF-1 at 537.489 kb on - strand, within trpF-1 at 537.574 kb on - strand, within trpF-1 at 537.579 kb on - strand, within trpF-1 at 537.664 kb on - strand at 537.671 kb on - strand at 537.688 kb on - strand at 537.690 kb on + strand at 537.692 kb on + strand at 538.023 kb on - strand, within trpB-2 at 538.233 kb on + strand, within trpB-2 at 538.236 kb on - strand, within trpB-2 at 538.244 kb on - strand, within trpB-2 at 538.254 kb on + strand, within trpB-2 at 538.264 kb on + strand, within trpB-2 at 538.432 kb on + strand, within trpB-2 at 538.527 kb on - strand, within trpB-2 at 538.529 kb on - strand, within trpB-2 at 538.594 kb on - strand, within trpB-2
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 535,871 - trpD DVU0467 0.81 -5.2 535,897 + trpD DVU0467 0.84 -3.9 535,905 - trpD DVU0467 0.85 -2.6 535,920 - trpD DVU0467 0.86 -4.8 535,938 + trpD DVU0467 0.88 -3.0 535,942 - trpD DVU0467 0.88 -1.1 535,942 - trpD DVU0467 0.88 -1.8 535,955 + trpD DVU0467 0.90 -1.8 535,955 + trpD DVU0467 0.90 -5.0 535,963 - -2.2 535,983 + -0.5 535,985 + -6.1 535,985 + +0.3 535,985 + -3.7 536,095 - -3.8 536,158 + trpC DVU0468 0.14 -5.5 536,164 - trpC DVU0468 0.15 -2.8 536,166 - trpC DVU0468 0.15 -3.5 536,166 - trpC DVU0468 0.15 -3.6 536,179 + trpC DVU0468 0.17 -4.0 536,179 + trpC DVU0468 0.17 -3.6 536,179 + trpC DVU0468 0.17 -3.5 536,301 - trpC DVU0468 0.33 -2.0 536,303 + trpC DVU0468 0.33 -1.9 536,311 - trpC DVU0468 0.34 -4.4 536,318 + trpC DVU0468 0.35 -4.1 536,318 + trpC DVU0468 0.35 -1.8 536,369 - trpC DVU0468 0.41 -3.5 536,425 + trpC DVU0468 0.49 -3.6 536,461 + trpC DVU0468 0.53 -5.9 536,467 + trpC DVU0468 0.54 -1.8 536,506 - trpC DVU0468 0.59 -3.4 536,511 + trpC DVU0468 0.60 -4.0 536,519 - trpC DVU0468 0.61 -1.8 536,519 - trpC DVU0468 0.61 -2.7 536,547 - trpC DVU0468 0.64 +0.7 536,566 + trpC DVU0468 0.67 -1.8 536,611 + trpC DVU0468 0.73 -4.5 536,619 - trpC DVU0468 0.74 -3.5 536,669 + trpC DVU0468 0.80 -3.7 536,750 - -4.0 536,757 + -2.3 536,757 + -1.0 536,765 - -3.7 536,799 + -0.7 536,807 - -3.4 536,860 + -1.3 536,979 + trpF-1 DVU0469 0.13 -4.6 537,037 - trpF-1 DVU0469 0.20 -2.6 537,037 - trpF-1 DVU0469 0.20 -5.0 537,044 - trpF-1 DVU0469 0.20 -3.7 537,044 - trpF-1 DVU0469 0.20 -4.3 537,135 + trpF-1 DVU0469 0.31 -3.4 537,206 - trpF-1 DVU0469 0.39 -1.7 537,288 + trpF-1 DVU0469 0.49 -3.9 537,389 - trpF-1 DVU0469 0.61 -4.5 537,476 + trpF-1 DVU0469 0.71 -0.7 537,479 - trpF-1 DVU0469 0.71 -4.3 537,489 - trpF-1 DVU0469 0.72 -1.1 537,574 - trpF-1 DVU0469 0.82 -3.2 537,579 - trpF-1 DVU0469 0.83 -0.7 537,664 - +1.7 537,671 - -0.1 537,688 - -0.1 537,690 + +0.2 537,692 + +0.2 538,023 - trpB-2 DVU0470 0.25 -0.3 538,233 + trpB-2 DVU0470 0.43 -2.6 538,236 - trpB-2 DVU0470 0.43 -0.6 538,244 - trpB-2 DVU0470 0.44 -3.5 538,254 + trpB-2 DVU0470 0.45 -2.3 538,264 + trpB-2 DVU0470 0.46 -2.6 538,432 + trpB-2 DVU0470 0.60 -2.1 538,527 - trpB-2 DVU0470 0.68 -2.3 538,529 - trpB-2 DVU0470 0.68 -4.6 538,594 - trpB-2 DVU0470 0.73 -3.5
Or see this region's nucleotide sequence