Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpE and trpG overlap by 20 nucleotides trpG and trpD overlap by 4 nucleotides trpD and trpC overlap by 8 nucleotides
DVU0465: trpE - anthranilate synthase, component I (TIGR), at 532,853 to 534,451
trpE
DVU0466: trpG - anthranilate synthase, glutamine amidotransferase component (TIGR), at 534,432 to 535,061
trpG
DVU0467: trpD - anthranilate phosphoribosyltransferase (TIGR), at 535,058 to 536,056
trpD
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
Position (kb) 534
535
536 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 533.461 kb on + strand, within trpE at 533.466 kb on + strand, within trpE at 533.489 kb on - strand, within trpE at 533.512 kb on + strand, within trpE at 533.547 kb on - strand, within trpE at 533.554 kb on - strand, within trpE at 533.608 kb on - strand, within trpE at 533.613 kb on - strand, within trpE at 533.615 kb on + strand, within trpE at 533.615 kb on + strand, within trpE at 533.623 kb on - strand, within trpE at 533.651 kb on - strand, within trpE at 533.687 kb on - strand, within trpE at 533.715 kb on - strand, within trpE at 533.719 kb on + strand, within trpE at 533.741 kb on - strand, within trpE at 533.743 kb on + strand, within trpE at 533.751 kb on - strand, within trpE at 533.769 kb on - strand, within trpE at 533.774 kb on + strand, within trpE at 533.871 kb on - strand, within trpE at 534.030 kb on - strand, within trpE at 534.037 kb on + strand, within trpE at 534.073 kb on + strand, within trpE at 534.090 kb on + strand, within trpE at 534.098 kb on - strand, within trpE at 534.107 kb on + strand, within trpE at 534.149 kb on + strand, within trpE at 534.180 kb on - strand, within trpE at 534.256 kb on - strand, within trpE at 534.322 kb on - strand at 534.382 kb on + strand at 534.418 kb on + strand at 534.466 kb on + strand at 534.490 kb on - strand at 534.500 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.501 kb on - strand, within trpG at 534.563 kb on + strand, within trpG at 534.596 kb on - strand, within trpG at 534.717 kb on - strand, within trpG at 534.766 kb on + strand, within trpG at 534.796 kb on + strand, within trpG at 534.807 kb on + strand, within trpG at 534.895 kb on - strand, within trpG at 534.905 kb on + strand, within trpG at 534.921 kb on - strand, within trpG at 534.935 kb on - strand, within trpG at 535.038 kb on - strand at 535.103 kb on + strand at 535.130 kb on + strand at 535.204 kb on + strand, within trpD at 535.206 kb on - strand, within trpD at 535.229 kb on - strand, within trpD at 535.233 kb on - strand, within trpD at 535.239 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.244 kb on + strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.252 kb on - strand, within trpD at 535.257 kb on - strand, within trpD at 535.272 kb on + strand, within trpD at 535.447 kb on - strand, within trpD at 535.508 kb on - strand, within trpD at 535.546 kb on + strand, within trpD at 535.591 kb on - strand, within trpD at 535.634 kb on - strand, within trpD at 535.709 kb on + strand, within trpD at 535.756 kb on - strand, within trpD at 535.839 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.841 kb on + strand, within trpD at 535.849 kb on - strand, within trpD at 535.871 kb on - strand, within trpD at 535.897 kb on + strand, within trpD at 535.905 kb on - strand, within trpD at 535.920 kb on - strand, within trpD at 535.938 kb on + strand, within trpD at 535.942 kb on - strand, within trpD at 535.942 kb on - strand, within trpD at 535.955 kb on + strand, within trpD at 535.955 kb on + strand, within trpD at 535.963 kb on - strand at 535.983 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand at 535.985 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 533,461 + trpE DVU0465 0.38 -2.8 533,466 + trpE DVU0465 0.38 -2.6 533,489 - trpE DVU0465 0.40 -2.6 533,512 + trpE DVU0465 0.41 -4.4 533,547 - trpE DVU0465 0.43 -3.0 533,554 - trpE DVU0465 0.44 -3.5 533,608 - trpE DVU0465 0.47 -5.0 533,613 - trpE DVU0465 0.48 -2.1 533,615 + trpE DVU0465 0.48 -3.0 533,615 + trpE DVU0465 0.48 -3.8 533,623 - trpE DVU0465 0.48 -3.7 533,651 - trpE DVU0465 0.50 -1.7 533,687 - trpE DVU0465 0.52 -4.3 533,715 - trpE DVU0465 0.54 -3.0 533,719 + trpE DVU0465 0.54 -3.1 533,741 - trpE DVU0465 0.56 -3.2 533,743 + trpE DVU0465 0.56 -2.4 533,751 - trpE DVU0465 0.56 -2.5 533,769 - trpE DVU0465 0.57 -3.7 533,774 + trpE DVU0465 0.58 -2.0 533,871 - trpE DVU0465 0.64 -3.9 534,030 - trpE DVU0465 0.74 -4.5 534,037 + trpE DVU0465 0.74 -1.7 534,073 + trpE DVU0465 0.76 -2.9 534,090 + trpE DVU0465 0.77 -4.2 534,098 - trpE DVU0465 0.78 -2.2 534,107 + trpE DVU0465 0.78 -4.1 534,149 + trpE DVU0465 0.81 -1.8 534,180 - trpE DVU0465 0.83 -4.4 534,256 - trpE DVU0465 0.88 -3.6 534,322 - -3.2 534,382 + -4.4 534,418 + -3.0 534,466 + -3.5 534,490 - -0.1 534,500 - trpG DVU0466 0.11 -3.5 534,501 - trpG DVU0466 0.11 -3.0 534,501 - trpG DVU0466 0.11 -3.3 534,563 + trpG DVU0466 0.21 -5.4 534,596 - trpG DVU0466 0.26 -3.5 534,717 - trpG DVU0466 0.45 -1.7 534,766 + trpG DVU0466 0.53 -0.8 534,796 + trpG DVU0466 0.58 -3.4 534,807 + trpG DVU0466 0.60 -3.1 534,895 - trpG DVU0466 0.73 -4.2 534,905 + trpG DVU0466 0.75 -1.6 534,921 - trpG DVU0466 0.78 -2.5 534,935 - trpG DVU0466 0.80 -1.1 535,038 - -1.3 535,103 + -4.5 535,130 + -2.4 535,204 + trpD DVU0467 0.15 -3.1 535,206 - trpD DVU0467 0.15 -4.7 535,229 - trpD DVU0467 0.17 -4.4 535,233 - trpD DVU0467 0.18 -3.1 535,239 + trpD DVU0467 0.18 -4.0 535,244 + trpD DVU0467 0.19 -3.8 535,244 + trpD DVU0467 0.19 -2.7 535,244 + trpD DVU0467 0.19 -4.3 535,244 + trpD DVU0467 0.19 -2.6 535,244 + trpD DVU0467 0.19 -4.8 535,252 - trpD DVU0467 0.19 -4.6 535,252 - trpD DVU0467 0.19 -2.1 535,252 - trpD DVU0467 0.19 -2.9 535,252 - trpD DVU0467 0.19 -0.8 535,257 - trpD DVU0467 0.20 -2.7 535,272 + trpD DVU0467 0.21 -2.8 535,447 - trpD DVU0467 0.39 -3.0 535,508 - trpD DVU0467 0.45 -4.6 535,546 + trpD DVU0467 0.49 -3.5 535,591 - trpD DVU0467 0.53 -3.6 535,634 - trpD DVU0467 0.58 -2.7 535,709 + trpD DVU0467 0.65 -2.5 535,756 - trpD DVU0467 0.70 -1.8 535,839 + trpD DVU0467 0.78 -1.9 535,841 + trpD DVU0467 0.78 -2.6 535,841 + trpD DVU0467 0.78 -1.1 535,849 - trpD DVU0467 0.79 -2.7 535,871 - trpD DVU0467 0.81 -5.2 535,897 + trpD DVU0467 0.84 -3.9 535,905 - trpD DVU0467 0.85 -2.6 535,920 - trpD DVU0467 0.86 -4.8 535,938 + trpD DVU0467 0.88 -3.0 535,942 - trpD DVU0467 0.88 -1.1 535,942 - trpD DVU0467 0.88 -1.8 535,955 + trpD DVU0467 0.90 -1.8 535,955 + trpD DVU0467 0.90 -5.0 535,963 - -2.2 535,983 + -0.5 535,985 + -6.1 535,985 + +0.3 535,985 + -3.7
Or see this region's nucleotide sequence