Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and DVU0045 are separated by 28 nucleotides DVU0045 and fliN overlap by 8 nucleotides fliN and DVU0047 are separated by 25 nucleotides DVU0047 and DVU0048 are separated by 20 nucleotides
DVU0044: fliP - flagellar biosynthetic protein fliP (TIGR), at 50,840 to 51,547
fliP
DVU0045: DVU0045 - flagellar biosynthesis protein, FliO, putative (TIGR), at 51,576 to 52,097
DVU0045
DVU0046: fliN - flagellar motor switch protein FliN (TIGR), at 52,090 to 52,602
fliN
DVU0047: DVU0047 - flagellar protein FliL (TIGR), at 52,628 to 53,143
DVU0047
DVU0048: DVU0048 - chemotaxis protein MotB (TIGR), at 53,164 to 53,904
DVU0048
Position (kb) 52
53 Strain fitness (log2 ratio) -2
-1
0
1 at 51.104 kb on - strand, within fliP at 51.220 kb on - strand, within fliP at 51.241 kb on - strand, within fliP at 51.243 kb on - strand, within fliP at 51.321 kb on - strand, within fliP at 51.390 kb on - strand, within fliP at 51.410 kb on + strand, within fliP at 51.467 kb on - strand, within fliP at 51.527 kb on - strand at 51.542 kb on - strand at 51.565 kb on + strand at 51.619 kb on + strand at 51.619 kb on - strand at 51.686 kb on + strand, within DVU0045 at 51.694 kb on - strand, within DVU0045 at 51.702 kb on + strand, within DVU0045 at 51.855 kb on + strand, within DVU0045 at 51.863 kb on - strand, within DVU0045 at 52.022 kb on - strand, within DVU0045 at 52.029 kb on - strand, within DVU0045 at 52.033 kb on - strand, within DVU0045 at 52.118 kb on + strand at 52.162 kb on + strand, within fliN at 52.212 kb on - strand, within fliN at 52.226 kb on + strand, within fliN at 52.236 kb on + strand, within fliN at 52.257 kb on - strand, within fliN at 52.267 kb on - strand, within fliN at 52.335 kb on + strand, within fliN at 52.391 kb on + strand, within fliN at 52.392 kb on - strand, within fliN at 52.476 kb on + strand, within fliN at 52.527 kb on + strand, within fliN at 52.528 kb on + strand, within fliN at 52.576 kb on + strand at 52.584 kb on - strand at 52.602 kb on + strand at 52.619 kb on + strand at 52.687 kb on + strand, within DVU0047 at 52.705 kb on + strand, within DVU0047 at 52.718 kb on - strand, within DVU0047 at 52.769 kb on - strand, within DVU0047 at 52.792 kb on + strand, within DVU0047 at 52.841 kb on - strand, within DVU0047 at 52.939 kb on - strand, within DVU0047 at 53.014 kb on - strand, within DVU0047 at 53.130 kb on + strand at 53.151 kb on + strand at 53.154 kb on - strand at 53.192 kb on + strand at 53.281 kb on + strand, within DVU0048 at 53.282 kb on + strand, within DVU0048 at 53.290 kb on - strand, within DVU0048 at 53.307 kb on - strand, within DVU0048 at 53.374 kb on + strand, within DVU0048 at 53.410 kb on - strand, within DVU0048 at 53.410 kb on - strand, within DVU0048 at 53.430 kb on + strand, within DVU0048 at 53.487 kb on - strand, within DVU0048 at 53.570 kb on - strand, within DVU0048 at 53.570 kb on - strand, within DVU0048 at 53.574 kb on + strand, within DVU0048 at 53.580 kb on + strand, within DVU0048 at 53.583 kb on - strand, within DVU0048 at 53.595 kb on - strand, within DVU0048 at 53.596 kb on - strand, within DVU0048
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 51,104 - fliP DVU0044 0.37 -0.8 51,220 - fliP DVU0044 0.54 -0.3 51,241 - fliP DVU0044 0.57 +0.1 51,243 - fliP DVU0044 0.57 +0.5 51,321 - fliP DVU0044 0.68 +0.5 51,390 - fliP DVU0044 0.78 +0.5 51,410 + fliP DVU0044 0.81 +0.4 51,467 - fliP DVU0044 0.89 +0.3 51,527 - +0.5 51,542 - -0.9 51,565 + +0.3 51,619 + +0.3 51,619 - -0.6 51,686 + DVU0045 0.21 +0.7 51,694 - DVU0045 0.23 +0.1 51,702 + DVU0045 0.24 +0.5 51,855 + DVU0045 0.53 +0.2 51,863 - DVU0045 0.55 +0.1 52,022 - DVU0045 0.85 +0.4 52,029 - DVU0045 0.87 +0.5 52,033 - DVU0045 0.88 +0.5 52,118 + +0.1 52,162 + fliN DVU0046 0.14 -0.3 52,212 - fliN DVU0046 0.24 -0.0 52,226 + fliN DVU0046 0.27 +0.1 52,236 + fliN DVU0046 0.28 +1.1 52,257 - fliN DVU0046 0.33 -0.1 52,267 - fliN DVU0046 0.35 +0.2 52,335 + fliN DVU0046 0.48 +0.4 52,391 + fliN DVU0046 0.59 -0.4 52,392 - fliN DVU0046 0.59 +1.1 52,476 + fliN DVU0046 0.75 -1.2 52,527 + fliN DVU0046 0.85 -0.2 52,528 + fliN DVU0046 0.85 +0.4 52,576 + +0.5 52,584 - +0.2 52,602 + -1.8 52,619 + -0.1 52,687 + DVU0047 0.11 +0.1 52,705 + DVU0047 0.15 -0.2 52,718 - DVU0047 0.17 +0.5 52,769 - DVU0047 0.27 +0.4 52,792 + DVU0047 0.32 +0.1 52,841 - DVU0047 0.41 -1.1 52,939 - DVU0047 0.60 -0.0 53,014 - DVU0047 0.75 -0.0 53,130 + +0.9 53,151 + -0.1 53,154 - -0.0 53,192 + +0.4 53,281 + DVU0048 0.16 -1.5 53,282 + DVU0048 0.16 +0.4 53,290 - DVU0048 0.17 -0.2 53,307 - DVU0048 0.19 +0.6 53,374 + DVU0048 0.28 +0.5 53,410 - DVU0048 0.33 -0.8 53,410 - DVU0048 0.33 -0.3 53,430 + DVU0048 0.36 +0.1 53,487 - DVU0048 0.44 -0.3 53,570 - DVU0048 0.55 -1.6 53,570 - DVU0048 0.55 +0.2 53,574 + DVU0048 0.55 +0.2 53,580 + DVU0048 0.56 -0.2 53,583 - DVU0048 0.57 +0.2 53,595 - DVU0048 0.58 +0.7 53,596 - DVU0048 0.58 -0.1
Or see this region's nucleotide sequence