Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliQ and fliP are separated by 16 nucleotides fliP and DVU0045 are separated by 28 nucleotides DVU0045 and fliN overlap by 8 nucleotides fliN and DVU0047 are separated by 25 nucleotides
DVU0043: fliQ - flagellar biosynthetic protein FliQ (TIGR), at 50,554 to 50,823
fliQ
DVU0044: fliP - flagellar biosynthetic protein fliP (TIGR), at 50,840 to 51,547
fliP
DVU0045: DVU0045 - flagellar biosynthesis protein, FliO, putative (TIGR), at 51,576 to 52,097
DVU0045
DVU0046: fliN - flagellar motor switch protein FliN (TIGR), at 52,090 to 52,602
fliN
DVU0047: DVU0047 - flagellar protein FliL (TIGR), at 52,628 to 53,143
DVU0047
Position (kb) 51
52
53 Strain fitness (log2 ratio) -2
-1
0
1 at 50.592 kb on + strand, within fliQ at 50.599 kb on - strand, within fliQ at 50.606 kb on + strand, within fliQ at 50.661 kb on + strand, within fliQ at 50.770 kb on - strand, within fliQ at 50.770 kb on - strand, within fliQ at 50.778 kb on + strand, within fliQ at 50.878 kb on + strand at 50.952 kb on - strand, within fliP at 50.986 kb on + strand, within fliP at 51.104 kb on - strand, within fliP at 51.220 kb on - strand, within fliP at 51.241 kb on - strand, within fliP at 51.243 kb on - strand, within fliP at 51.321 kb on - strand, within fliP at 51.390 kb on - strand, within fliP at 51.410 kb on + strand, within fliP at 51.467 kb on - strand, within fliP at 51.527 kb on - strand at 51.542 kb on - strand at 51.565 kb on + strand at 51.619 kb on + strand at 51.619 kb on - strand at 51.686 kb on + strand, within DVU0045 at 51.694 kb on - strand, within DVU0045 at 51.702 kb on + strand, within DVU0045 at 51.855 kb on + strand, within DVU0045 at 51.863 kb on - strand, within DVU0045 at 52.022 kb on - strand, within DVU0045 at 52.029 kb on - strand, within DVU0045 at 52.033 kb on - strand, within DVU0045 at 52.118 kb on + strand at 52.162 kb on + strand, within fliN at 52.212 kb on - strand, within fliN at 52.226 kb on + strand, within fliN at 52.236 kb on + strand, within fliN at 52.257 kb on - strand, within fliN at 52.267 kb on - strand, within fliN at 52.335 kb on + strand, within fliN at 52.391 kb on + strand, within fliN at 52.392 kb on - strand, within fliN at 52.476 kb on + strand, within fliN at 52.527 kb on + strand, within fliN at 52.528 kb on + strand, within fliN at 52.576 kb on + strand at 52.584 kb on - strand at 52.602 kb on + strand at 52.619 kb on + strand at 52.687 kb on + strand, within DVU0047 at 52.705 kb on + strand, within DVU0047 at 52.718 kb on - strand, within DVU0047 at 52.769 kb on - strand, within DVU0047 at 52.792 kb on + strand, within DVU0047 at 52.841 kb on - strand, within DVU0047 at 52.939 kb on - strand, within DVU0047 at 53.014 kb on - strand, within DVU0047
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 50,592 + fliQ DVU0043 0.14 -0.4 50,599 - fliQ DVU0043 0.17 +0.5 50,606 + fliQ DVU0043 0.19 -0.1 50,661 + fliQ DVU0043 0.40 +0.7 50,770 - fliQ DVU0043 0.80 +0.5 50,770 - fliQ DVU0043 0.80 +0.3 50,778 + fliQ DVU0043 0.83 +0.7 50,878 + +0.7 50,952 - fliP DVU0044 0.16 -0.6 50,986 + fliP DVU0044 0.21 +1.0 51,104 - fliP DVU0044 0.37 -0.8 51,220 - fliP DVU0044 0.54 -0.3 51,241 - fliP DVU0044 0.57 +0.1 51,243 - fliP DVU0044 0.57 +0.5 51,321 - fliP DVU0044 0.68 +0.5 51,390 - fliP DVU0044 0.78 +0.5 51,410 + fliP DVU0044 0.81 +0.4 51,467 - fliP DVU0044 0.89 +0.3 51,527 - +0.5 51,542 - -0.9 51,565 + +0.3 51,619 + +0.3 51,619 - -0.6 51,686 + DVU0045 0.21 +0.7 51,694 - DVU0045 0.23 +0.1 51,702 + DVU0045 0.24 +0.5 51,855 + DVU0045 0.53 +0.2 51,863 - DVU0045 0.55 +0.1 52,022 - DVU0045 0.85 +0.4 52,029 - DVU0045 0.87 +0.5 52,033 - DVU0045 0.88 +0.5 52,118 + +0.1 52,162 + fliN DVU0046 0.14 -0.3 52,212 - fliN DVU0046 0.24 -0.0 52,226 + fliN DVU0046 0.27 +0.1 52,236 + fliN DVU0046 0.28 +1.1 52,257 - fliN DVU0046 0.33 -0.1 52,267 - fliN DVU0046 0.35 +0.2 52,335 + fliN DVU0046 0.48 +0.4 52,391 + fliN DVU0046 0.59 -0.4 52,392 - fliN DVU0046 0.59 +1.1 52,476 + fliN DVU0046 0.75 -1.2 52,527 + fliN DVU0046 0.85 -0.2 52,528 + fliN DVU0046 0.85 +0.4 52,576 + +0.5 52,584 - +0.2 52,602 + -1.8 52,619 + -0.1 52,687 + DVU0047 0.11 +0.1 52,705 + DVU0047 0.15 -0.2 52,718 - DVU0047 0.17 +0.5 52,769 - DVU0047 0.27 +0.4 52,792 + DVU0047 0.32 +0.1 52,841 - DVU0047 0.41 -1.1 52,939 - DVU0047 0.60 -0.0 53,014 - DVU0047 0.75 -0.0
Or see this region's nucleotide sequence