Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3349 and oorD are separated by 3 nucleotides oorD and queA are separated by 363 nucleotides queA and DVU3352 are separated by 112 nucleotides DVU3352 and coaBC overlap by 1 nucleotides
DVU3349: DVU3349 - pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-binding domain protein (TIGR), at 3,520,596 to 3,521,669
DVU3349
DVU3350: oorD - iron-sulfur cluster-binding protein (TIGR), at 3,521,673 to 3,521,906
oorD
DVU3351: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (TIGR), at 3,522,270 to 3,523,385
queA
DVU3352: DVU3352 - lipoprotein, putative (TIGR), at 3,523,498 to 3,524,067
DVU3352
DVU3353: coaBC - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase (TIGR), at 3,524,067 to 3,525,281
coaBC
Position (kb)
3522
3523
3524 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3521.403 kb on - strand, within DVU3349 at 3521.691 kb on - strand at 3521.716 kb on - strand, within oorD at 3521.745 kb on + strand, within oorD at 3521.851 kb on + strand, within oorD at 3521.854 kb on + strand, within oorD at 3521.854 kb on + strand, within oorD at 3521.859 kb on - strand, within oorD at 3522.051 kb on - strand at 3522.051 kb on - strand at 3522.120 kb on - strand at 3522.122 kb on - strand at 3522.122 kb on - strand at 3522.137 kb on + strand at 3522.137 kb on + strand at 3522.196 kb on + strand at 3522.209 kb on + strand at 3522.209 kb on + strand at 3522.217 kb on - strand at 3522.246 kb on + strand at 3522.254 kb on - strand at 3522.266 kb on + strand at 3522.319 kb on - strand at 3522.366 kb on + strand at 3522.367 kb on + strand at 3522.375 kb on - strand at 3522.400 kb on - strand, within queA at 3522.401 kb on + strand, within queA at 3522.439 kb on - strand, within queA at 3522.480 kb on + strand, within queA at 3522.488 kb on - strand, within queA at 3522.488 kb on - strand, within queA at 3522.490 kb on - strand, within queA at 3522.490 kb on - strand, within queA at 3522.492 kb on + strand, within queA at 3522.513 kb on + strand, within queA at 3522.530 kb on + strand, within queA at 3522.558 kb on + strand, within queA at 3522.558 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.565 kb on + strand, within queA at 3522.592 kb on - strand, within queA at 3522.592 kb on - strand, within queA at 3522.621 kb on + strand, within queA at 3522.653 kb on + strand, within queA at 3522.671 kb on - strand, within queA at 3522.680 kb on + strand, within queA at 3522.680 kb on + strand, within queA at 3522.688 kb on - strand, within queA at 3522.692 kb on + strand, within queA at 3522.694 kb on - strand, within queA at 3522.786 kb on + strand, within queA at 3522.813 kb on - strand, within queA at 3522.822 kb on + strand, within queA at 3522.832 kb on - strand, within queA at 3522.887 kb on - strand, within queA at 3522.912 kb on - strand, within queA at 3522.917 kb on + strand, within queA at 3522.925 kb on - strand, within queA at 3522.935 kb on - strand, within queA at 3522.945 kb on - strand, within queA at 3522.958 kb on + strand, within queA at 3522.959 kb on + strand, within queA at 3522.971 kb on + strand, within queA at 3523.004 kb on + strand, within queA at 3523.012 kb on - strand, within queA at 3523.022 kb on + strand, within queA at 3523.032 kb on - strand, within queA at 3523.032 kb on - strand, within queA at 3523.052 kb on + strand, within queA at 3523.057 kb on + strand, within queA at 3523.067 kb on + strand, within queA at 3523.126 kb on + strand, within queA at 3523.127 kb on - strand, within queA at 3523.144 kb on - strand, within queA at 3523.179 kb on - strand, within queA at 3523.260 kb on - strand, within queA at 3523.305 kb on + strand at 3523.338 kb on - strand at 3523.339 kb on - strand at 3523.346 kb on - strand at 3523.374 kb on - strand at 3523.428 kb on - strand at 3523.435 kb on + strand at 3523.444 kb on + strand at 3523.444 kb on + strand at 3523.444 kb on + strand at 3523.456 kb on + strand at 3523.488 kb on + strand at 3523.496 kb on - strand at 3523.535 kb on + strand at 3523.574 kb on + strand, within DVU3352 at 3523.635 kb on + strand, within DVU3352 at 3523.675 kb on + strand, within DVU3352 at 3523.827 kb on + strand, within DVU3352 at 3523.827 kb on + strand, within DVU3352 at 3523.901 kb on + strand, within DVU3352 at 3523.901 kb on + strand, within DVU3352 at 3523.959 kb on + strand, within DVU3352 at 3524.064 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 3,521,403 - DVU3349 0.75 -1.0 3,521,691 - -0.5 3,521,716 - oorD DVU3350 0.18 +0.0 3,521,745 + oorD DVU3350 0.31 -1.7 3,521,851 + oorD DVU3350 0.76 -1.9 3,521,854 + oorD DVU3350 0.77 -2.5 3,521,854 + oorD DVU3350 0.77 -0.0 3,521,859 - oorD DVU3350 0.79 -0.9 3,522,051 - -0.3 3,522,051 - -0.0 3,522,120 - -0.2 3,522,122 - +0.8 3,522,122 - -0.2 3,522,137 + -1.2 3,522,137 + -0.9 3,522,196 + -0.3 3,522,209 + -2.4 3,522,209 + -0.9 3,522,217 - +0.4 3,522,246 + +0.7 3,522,254 - -0.1 3,522,266 + -0.2 3,522,319 - +0.2 3,522,366 + +0.2 3,522,367 + +1.3 3,522,375 - +0.2 3,522,400 - queA DVU3351 0.12 -0.3 3,522,401 + queA DVU3351 0.12 +0.5 3,522,439 - queA DVU3351 0.15 +1.3 3,522,480 + queA DVU3351 0.19 -0.4 3,522,488 - queA DVU3351 0.20 +0.4 3,522,488 - queA DVU3351 0.20 +0.6 3,522,490 - queA DVU3351 0.20 +0.5 3,522,490 - queA DVU3351 0.20 -0.9 3,522,492 + queA DVU3351 0.20 +0.6 3,522,513 + queA DVU3351 0.22 -0.1 3,522,530 + queA DVU3351 0.23 -0.0 3,522,558 + queA DVU3351 0.26 -0.2 3,522,558 + queA DVU3351 0.26 -0.2 3,522,565 + queA DVU3351 0.26 +0.1 3,522,565 + queA DVU3351 0.26 -0.6 3,522,565 + queA DVU3351 0.26 +0.5 3,522,592 - queA DVU3351 0.29 -0.3 3,522,592 - queA DVU3351 0.29 +0.1 3,522,621 + queA DVU3351 0.31 +0.0 3,522,653 + queA DVU3351 0.34 -2.3 3,522,671 - queA DVU3351 0.36 +1.8 3,522,680 + queA DVU3351 0.37 -0.2 3,522,680 + queA DVU3351 0.37 -0.2 3,522,688 - queA DVU3351 0.37 -1.1 3,522,692 + queA DVU3351 0.38 +0.1 3,522,694 - queA DVU3351 0.38 +0.6 3,522,786 + queA DVU3351 0.46 -0.1 3,522,813 - queA DVU3351 0.49 +0.2 3,522,822 + queA DVU3351 0.49 +0.5 3,522,832 - queA DVU3351 0.50 +0.3 3,522,887 - queA DVU3351 0.55 +0.1 3,522,912 - queA DVU3351 0.58 -1.1 3,522,917 + queA DVU3351 0.58 +0.3 3,522,925 - queA DVU3351 0.59 -0.3 3,522,935 - queA DVU3351 0.60 +0.8 3,522,945 - queA DVU3351 0.60 -0.1 3,522,958 + queA DVU3351 0.62 +0.2 3,522,959 + queA DVU3351 0.62 +0.0 3,522,971 + queA DVU3351 0.63 +0.6 3,523,004 + queA DVU3351 0.66 -0.0 3,523,012 - queA DVU3351 0.66 +0.4 3,523,022 + queA DVU3351 0.67 -0.1 3,523,032 - queA DVU3351 0.68 -0.0 3,523,032 - queA DVU3351 0.68 -0.3 3,523,052 + queA DVU3351 0.70 +1.5 3,523,057 + queA DVU3351 0.71 -0.7 3,523,067 + queA DVU3351 0.71 -0.5 3,523,126 + queA DVU3351 0.77 +1.2 3,523,127 - queA DVU3351 0.77 -0.3 3,523,144 - queA DVU3351 0.78 +0.2 3,523,179 - queA DVU3351 0.81 +1.3 3,523,260 - queA DVU3351 0.89 +0.2 3,523,305 + -0.4 3,523,338 - +1.2 3,523,339 - +0.2 3,523,346 - -0.0 3,523,374 - +0.1 3,523,428 - -0.1 3,523,435 + -2.4 3,523,444 + +0.5 3,523,444 + -0.5 3,523,444 + +0.5 3,523,456 + -0.2 3,523,488 + +0.4 3,523,496 - -1.8 3,523,535 + -0.1 3,523,574 + DVU3352 0.13 -0.2 3,523,635 + DVU3352 0.24 +0.1 3,523,675 + DVU3352 0.31 +0.1 3,523,827 + DVU3352 0.58 +0.1 3,523,827 + DVU3352 0.58 +0.5 3,523,901 + DVU3352 0.71 +0.4 3,523,901 + DVU3352 0.71 -0.4 3,523,959 + DVU3352 0.81 -0.3 3,524,064 + -1.8
Or see this region's nucleotide sequence