Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3226 and DVU3227 are separated by 1 nucleotides DVU3227 and cheY-3 are separated by 75 nucleotides cheY-3 and fliA are separated by 22 nucleotides fliA and DVU3230 overlap by 47 nucleotides
DVU3226: DVU3226 - hypothetical protein (TIGR), at 3,395,327 to 3,395,656
DVU3226
DVU3227: DVU3227 - flagellar basal body-associated protein, putative (TIGR), at 3,395,658 to 3,396,341
DVU3227
DVU3228: cheY-3 - chemotaxis protein CheY (TIGR), at 3,396,417 to 3,396,797
cheY-3
DVU3229: fliA - RNA polymerase sigma factor for flagellar operon FliA (TIGR), at 3,396,820 to 3,397,617
fliA
DVU3230: DVU3230 - flagellar synthesis regulator FleN (TIGR), at 3,397,571 to 3,398,398
DVU3230
Position (kb)
3396
3397 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3395.450 kb on + strand, within DVU3226 at 3395.587 kb on - strand, within DVU3226 at 3395.676 kb on - strand at 3395.676 kb on - strand at 3395.862 kb on - strand, within DVU3227 at 3395.937 kb on - strand, within DVU3227 at 3395.967 kb on - strand, within DVU3227 at 3396.004 kb on + strand, within DVU3227 at 3396.010 kb on + strand, within DVU3227 at 3396.012 kb on + strand, within DVU3227 at 3396.018 kb on - strand, within DVU3227 at 3396.077 kb on - strand, within DVU3227 at 3396.106 kb on + strand, within DVU3227 at 3396.134 kb on - strand, within DVU3227 at 3396.304 kb on + strand at 3396.310 kb on + strand at 3396.312 kb on - strand at 3396.443 kb on + strand at 3396.510 kb on - strand, within cheY-3 at 3396.566 kb on + strand, within cheY-3 at 3396.602 kb on + strand, within cheY-3 at 3396.646 kb on - strand, within cheY-3 at 3396.651 kb on - strand, within cheY-3 at 3396.673 kb on + strand, within cheY-3 at 3396.795 kb on + strand at 3396.797 kb on + strand at 3396.813 kb on - strand at 3396.817 kb on + strand at 3396.825 kb on - strand at 3396.825 kb on - strand at 3396.839 kb on + strand at 3396.876 kb on + strand at 3396.879 kb on + strand at 3397.086 kb on - strand, within fliA at 3397.262 kb on - strand, within fliA at 3397.401 kb on + strand, within fliA at 3397.417 kb on - strand, within fliA at 3397.417 kb on - strand, within fliA at 3397.436 kb on - strand, within fliA at 3397.453 kb on - strand, within fliA at 3397.459 kb on - strand, within fliA at 3397.472 kb on - strand, within fliA at 3397.513 kb on - strand, within fliA at 3397.610 kb on - strand at 3397.669 kb on + strand, within DVU3230
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 3,395,450 + DVU3226 0.37 -0.6 3,395,587 - DVU3226 0.79 -0.1 3,395,676 - -0.2 3,395,676 - +0.1 3,395,862 - DVU3227 0.30 -0.4 3,395,937 - DVU3227 0.41 +0.1 3,395,967 - DVU3227 0.45 -0.7 3,396,004 + DVU3227 0.51 -0.8 3,396,010 + DVU3227 0.51 +0.3 3,396,012 + DVU3227 0.52 -0.4 3,396,018 - DVU3227 0.53 +0.3 3,396,077 - DVU3227 0.61 -0.0 3,396,106 + DVU3227 0.65 +0.2 3,396,134 - DVU3227 0.70 -0.0 3,396,304 + +0.3 3,396,310 + -0.4 3,396,312 - +0.1 3,396,443 + -0.2 3,396,510 - cheY-3 DVU3228 0.24 -0.6 3,396,566 + cheY-3 DVU3228 0.39 -1.1 3,396,602 + cheY-3 DVU3228 0.49 +0.1 3,396,646 - cheY-3 DVU3228 0.60 -1.2 3,396,651 - cheY-3 DVU3228 0.61 +1.2 3,396,673 + cheY-3 DVU3228 0.67 -2.1 3,396,795 + -0.0 3,396,797 + -0.1 3,396,813 - -0.8 3,396,817 + -1.5 3,396,825 - +0.8 3,396,825 - -0.8 3,396,839 + +0.3 3,396,876 + -0.2 3,396,879 + +0.6 3,397,086 - fliA DVU3229 0.33 +0.1 3,397,262 - fliA DVU3229 0.55 -0.0 3,397,401 + fliA DVU3229 0.73 +0.9 3,397,417 - fliA DVU3229 0.75 +0.8 3,397,417 - fliA DVU3229 0.75 +0.2 3,397,436 - fliA DVU3229 0.77 +0.4 3,397,453 - fliA DVU3229 0.79 -0.2 3,397,459 - fliA DVU3229 0.80 -0.2 3,397,472 - fliA DVU3229 0.82 +0.1 3,397,513 - fliA DVU3229 0.87 -0.0 3,397,610 - -3.0 3,397,669 + DVU3230 0.12 -1.5
Or see this region's nucleotide sequence