Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysS and tRNA-Asn are separated by 190 nucleotides tRNA-Asn and DVU2377 are separated by 347 nucleotides DVU2377 and foxR are separated by 109 nucleotides foxR and pqqL are separated by 124 nucleotides
DVU2376: lysS - lysyl-tRNA synthetase (TIGR), at 2,473,176 to 2,474,759
lysS
DVU_tRNA-Asn-1: tRNA-Asn - tRNA with anticodon GTT for Asn (VIMSS-AUTO), at 2,474,950 to 2,475,025
tRNA-Asn
DVU2377: DVU2377 - hypothetical protein (TIGR), at 2,475,373 to 2,475,504
DVU2377
DVU2378: foxR - transcriptional regulator, AraC family (TIGR), at 2,475,614 to 2,476,306
foxR
DVU2379: pqqL - peptidase, M16 family, putative (TIGR), at 2,476,431 to 2,479,448
pqqL
Position (kb)
2475
2476 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2474.924 kb on + strand at 2475.136 kb on - strand at 2475.158 kb on - strand at 2475.165 kb on + strand at 2475.173 kb on - strand at 2475.235 kb on - strand at 2475.254 kb on - strand at 2475.255 kb on + strand at 2475.255 kb on + strand at 2475.256 kb on - strand at 2475.258 kb on + strand at 2475.261 kb on - strand at 2475.266 kb on - strand at 2475.472 kb on + strand, within DVU2377 at 2475.490 kb on - strand, within DVU2377 at 2475.557 kb on - strand at 2475.614 kb on + strand at 2475.624 kb on + strand at 2475.658 kb on - strand at 2475.668 kb on + strand at 2475.671 kb on - strand at 2475.696 kb on + strand, within foxR at 2475.704 kb on - strand, within foxR at 2475.712 kb on + strand, within foxR at 2475.726 kb on + strand, within foxR at 2475.780 kb on - strand, within foxR at 2475.802 kb on + strand, within foxR at 2475.868 kb on - strand, within foxR at 2475.904 kb on - strand, within foxR at 2475.914 kb on + strand, within foxR at 2475.917 kb on - strand, within foxR at 2475.939 kb on - strand, within foxR at 2476.136 kb on + strand, within foxR at 2476.145 kb on + strand, within foxR at 2476.189 kb on + strand, within foxR at 2476.262 kb on + strand at 2476.433 kb on - strand at 2476.433 kb on - strand at 2476.454 kb on + strand at 2476.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 2,474,924 + -1.8 2,475,136 - -0.9 2,475,158 - -1.0 2,475,165 + -0.1 2,475,173 - -0.2 2,475,235 - -0.1 2,475,254 - -0.1 2,475,255 + -0.1 2,475,255 + -0.4 2,475,256 - +1.8 2,475,258 + -0.9 2,475,261 - +0.3 2,475,266 - -1.7 2,475,472 + DVU2377 0.75 +0.0 2,475,490 - DVU2377 0.89 -0.0 2,475,557 - -0.7 2,475,614 + -1.1 2,475,624 + -0.3 2,475,658 - -0.4 2,475,668 + -0.3 2,475,671 - +0.3 2,475,696 + foxR DVU2378 0.12 -1.0 2,475,704 - foxR DVU2378 0.13 -1.2 2,475,712 + foxR DVU2378 0.14 -0.4 2,475,726 + foxR DVU2378 0.16 -1.3 2,475,780 - foxR DVU2378 0.24 -0.0 2,475,802 + foxR DVU2378 0.27 +0.4 2,475,868 - foxR DVU2378 0.37 -0.2 2,475,904 - foxR DVU2378 0.42 -0.1 2,475,914 + foxR DVU2378 0.43 -0.8 2,475,917 - foxR DVU2378 0.44 -0.2 2,475,939 - foxR DVU2378 0.47 -0.8 2,476,136 + foxR DVU2378 0.75 +0.1 2,476,145 + foxR DVU2378 0.77 +0.1 2,476,189 + foxR DVU2378 0.83 -0.2 2,476,262 + +1.3 2,476,433 - +0.0 2,476,433 - -0.2 2,476,454 + +0.3 2,476,490 - +0.2
Or see this region's nucleotide sequence