Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2359 and DVU2360 are separated by 103 nucleotides DVU2360 and thiE-2 are separated by 223 nucleotides thiE-2 and thiM overlap by 4 nucleotides
DVU2359: DVU2359 - sigma-54 dependent transcriptional regulator (TIGR), at 2,456,656 to 2,458,311
DVU2359
DVU2360: DVU2360 - oxidoreductase, FAD/NAD-binding family (TIGR), at 2,458,415 to 2,459,221
DVU2360
DVU2362: thiE-2 - thiamine-phosphate pyrophosphorylase (TIGR), at 2,459,445 to 2,460,104
thiE-2
DVU2363: thiM - hydroxyethylthiazole kinase (TIGR), at 2,460,101 to 2,460,904
thiM
Position (kb)
2458
2459
2460 Strain fitness (log2 ratio)
-2
-1
0
1 at 2457.522 kb on + strand, within DVU2359 at 2457.528 kb on - strand, within DVU2359 at 2457.530 kb on - strand, within DVU2359 at 2457.674 kb on + strand, within DVU2359 at 2457.722 kb on + strand, within DVU2359 at 2457.902 kb on - strand, within DVU2359 at 2457.920 kb on + strand, within DVU2359 at 2457.951 kb on + strand, within DVU2359 at 2457.951 kb on + strand, within DVU2359 at 2458.022 kb on - strand, within DVU2359 at 2458.040 kb on + strand, within DVU2359 at 2458.045 kb on + strand, within DVU2359 at 2458.087 kb on - strand, within DVU2359 at 2458.093 kb on - strand, within DVU2359 at 2458.230 kb on - strand at 2458.369 kb on + strand at 2458.375 kb on - strand at 2458.425 kb on - strand at 2458.528 kb on + strand, within DVU2360 at 2458.558 kb on + strand, within DVU2360 at 2458.627 kb on + strand, within DVU2360 at 2458.634 kb on + strand, within DVU2360 at 2458.637 kb on - strand, within DVU2360 at 2458.677 kb on - strand, within DVU2360 at 2458.711 kb on - strand, within DVU2360 at 2458.801 kb on + strand, within DVU2360 at 2458.839 kb on - strand, within DVU2360 at 2458.895 kb on + strand, within DVU2360 at 2458.908 kb on - strand, within DVU2360 at 2458.930 kb on + strand, within DVU2360 at 2458.965 kb on + strand, within DVU2360 at 2458.973 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.086 kb on - strand, within DVU2360 at 2459.121 kb on - strand, within DVU2360 at 2459.180 kb on + strand at 2459.188 kb on - strand at 2459.204 kb on + strand at 2459.229 kb on + strand at 2459.237 kb on - strand at 2459.237 kb on - strand at 2459.329 kb on + strand at 2459.369 kb on + strand at 2459.370 kb on + strand at 2459.403 kb on - strand at 2459.440 kb on - strand at 2459.485 kb on - strand at 2459.578 kb on + strand, within thiE-2 at 2459.578 kb on + strand, within thiE-2 at 2459.727 kb on + strand, within thiE-2 at 2459.748 kb on - strand, within thiE-2 at 2459.978 kb on + strand, within thiE-2 at 2460.052 kb on - strand at 2460.061 kb on + strand at 2460.103 kb on - strand at 2460.122 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 2,457,522 + DVU2359 0.52 -0.2 2,457,528 - DVU2359 0.53 -0.5 2,457,530 - DVU2359 0.53 -0.2 2,457,674 + DVU2359 0.61 -0.9 2,457,722 + DVU2359 0.64 +1.2 2,457,902 - DVU2359 0.75 -0.2 2,457,920 + DVU2359 0.76 +0.4 2,457,951 + DVU2359 0.78 -0.0 2,457,951 + DVU2359 0.78 +0.6 2,458,022 - DVU2359 0.82 +0.4 2,458,040 + DVU2359 0.84 -0.1 2,458,045 + DVU2359 0.84 -2.0 2,458,087 - DVU2359 0.86 -0.5 2,458,093 - DVU2359 0.87 -0.1 2,458,230 - -0.5 2,458,369 + -0.3 2,458,375 - -0.3 2,458,425 - -0.2 2,458,528 + DVU2360 0.14 -0.0 2,458,558 + DVU2360 0.18 -1.6 2,458,627 + DVU2360 0.26 -0.0 2,458,634 + DVU2360 0.27 -0.4 2,458,637 - DVU2360 0.28 -0.0 2,458,677 - DVU2360 0.32 +0.0 2,458,711 - DVU2360 0.37 -0.5 2,458,801 + DVU2360 0.48 +0.1 2,458,839 - DVU2360 0.53 +0.3 2,458,895 + DVU2360 0.59 -0.7 2,458,908 - DVU2360 0.61 +0.3 2,458,930 + DVU2360 0.64 -0.1 2,458,965 + DVU2360 0.68 -0.2 2,458,973 - DVU2360 0.69 +1.2 2,459,022 - DVU2360 0.75 +0.2 2,459,022 - DVU2360 0.75 +0.6 2,459,022 - DVU2360 0.75 +0.1 2,459,086 - DVU2360 0.83 -0.4 2,459,121 - DVU2360 0.87 +1.6 2,459,180 + -0.9 2,459,188 - +0.8 2,459,204 + -0.2 2,459,229 + -0.1 2,459,237 - -0.2 2,459,237 - -2.4 2,459,329 + +0.2 2,459,369 + +0.2 2,459,370 + +1.3 2,459,403 - -1.7 2,459,440 - -0.3 2,459,485 - -0.7 2,459,578 + thiE-2 DVU2362 0.20 +0.1 2,459,578 + thiE-2 DVU2362 0.20 -0.3 2,459,727 + thiE-2 DVU2362 0.43 -0.1 2,459,748 - thiE-2 DVU2362 0.46 +0.3 2,459,978 + thiE-2 DVU2362 0.81 -0.7 2,460,052 - -0.3 2,460,061 + -0.2 2,460,103 - -0.3 2,460,122 - -0.4
Or see this region's nucleotide sequence