Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2326 and DORF17147 overlap by 1 nucleotides DORF17147 and hypA are separated by 273 nucleotides hypA and hypB are separated by 10 nucleotides hypB and E.coliMinDlike) overlap by 4 nucleotides
DVU2326: DVU2326 - conserved domain protein (TIGR), at 2,422,922 to 2,423,824
DVU2326
DORF17147: DORF17147 - SEED: TPR domain protein, at 2,423,824 to 2,424,540
DORF17147
DVU2328: hypA - hydrogenase nickel insertion protein HypA, putative (TIGR), at 2,424,814 to 2,425,167
hypA
DVU2329: hypB - hydrogenase accessory protein HypB (TIGR), at 2,425,178 to 2,425,852
hypB
DVU2330: E.coliMinDlike) - MRP family protein (TIGR), at 2,425,849 to 2,426,730
E.coliMinDlike)
Position (kb)
2424
2425
2426 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2423.960 kb on - strand, within DORF17147 at 2424.014 kb on + strand, within DORF17147 at 2424.080 kb on + strand, within DORF17147 at 2424.145 kb on + strand, within DORF17147 at 2424.320 kb on - strand, within DORF17147 at 2424.389 kb on + strand, within DORF17147 at 2424.402 kb on - strand, within DORF17147 at 2424.411 kb on + strand, within DORF17147 at 2424.437 kb on + strand, within DORF17147 at 2424.449 kb on + strand, within DORF17147 at 2424.464 kb on + strand, within DORF17147 at 2424.464 kb on + strand, within DORF17147 at 2424.473 kb on + strand at 2424.511 kb on - strand at 2424.558 kb on + strand at 2424.566 kb on + strand at 2424.612 kb on + strand at 2424.620 kb on - strand at 2424.720 kb on + strand at 2424.748 kb on + strand at 2424.785 kb on + strand at 2424.817 kb on + strand at 2424.840 kb on - strand at 2424.953 kb on + strand, within hypA at 2425.004 kb on - strand, within hypA at 2425.064 kb on + strand, within hypA at 2425.123 kb on + strand, within hypA at 2425.152 kb on + strand at 2425.813 kb on + strand at 2425.854 kb on - strand at 2425.854 kb on - strand at 2425.890 kb on - strand at 2425.917 kb on + strand at 2425.925 kb on - strand at 2426.016 kb on - strand, within E.coliMinDlike) at 2426.050 kb on - strand, within E.coliMinDlike) at 2426.110 kb on - strand, within E.coliMinDlike)
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 2,423,960 - DORF17147 0.19 +0.3 2,424,014 + DORF17147 0.26 -0.3 2,424,080 + DORF17147 0.36 -0.1 2,424,145 + DORF17147 0.45 -0.2 2,424,320 - DORF17147 0.69 +0.5 2,424,389 + DORF17147 0.79 +0.1 2,424,402 - DORF17147 0.81 -0.4 2,424,411 + DORF17147 0.82 -0.1 2,424,437 + DORF17147 0.85 +0.3 2,424,449 + DORF17147 0.87 +0.4 2,424,464 + DORF17147 0.89 -0.5 2,424,464 + DORF17147 0.89 -0.0 2,424,473 + +0.1 2,424,511 - +0.5 2,424,558 + -0.3 2,424,566 + -0.0 2,424,612 + +0.1 2,424,620 - +0.1 2,424,720 + -0.1 2,424,748 + -0.2 2,424,785 + -1.0 2,424,817 + -0.4 2,424,840 - -0.1 2,424,953 + hypA DVU2328 0.39 +0.3 2,425,004 - hypA DVU2328 0.54 -1.1 2,425,064 + hypA DVU2328 0.71 -1.0 2,425,123 + hypA DVU2328 0.87 -1.1 2,425,152 + +0.4 2,425,813 + -0.6 2,425,854 - -2.1 2,425,854 - -0.7 2,425,890 - -0.2 2,425,917 + +2.0 2,425,925 - +1.3 2,426,016 - E.coliMinDlike) DVU2330 0.19 -0.3 2,426,050 - E.coliMinDlike) DVU2330 0.23 +0.3 2,426,110 - E.coliMinDlike) DVU2330 0.30 +0.3
Or see this region's nucleotide sequence