Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2149 and dksA are separated by 19 nucleotides dksA and tRNA-Gly are separated by 745 nucleotides tRNA-Gly and tRNA-Cys are separated by 11 nucleotides tRNA-Cys and tRNA-Gly are separated by 7 nucleotides
DVU2149: DVU2149 - conserved hypothetical protein (TIGR), at 2,247,518 to 2,249,257
DVU2149
DVU2150: dksA - dnaK suppressor protein, putative (TIGR), at 2,249,277 to 2,249,639
dksA
DVU_tRNA-Gly-1: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,385 to 2,250,459
tRNA-Gly
DVU_tRNA-Cys-1: tRNA-Cys - tRNA with anticodon GCA for Cys (VIMSS-AUTO), at 2,250,471 to 2,250,545
tRNA-Cys
DVU_tRNA-Gly-2: tRNA-Gly - tRNA with anticodon GCC for Gly (VIMSS-AUTO), at 2,250,553 to 2,250,627
tRNA-Gly
Position (kb)
2249
2250 Strain fitness (log2 ratio)
-2
-1
0
1 at 2248.277 kb on - strand, within DVU2149 at 2248.328 kb on - strand, within DVU2149 at 2248.390 kb on - strand, within DVU2149 at 2248.422 kb on + strand, within DVU2149 at 2248.473 kb on + strand, within DVU2149 at 2248.473 kb on + strand, within DVU2149 at 2248.484 kb on + strand, within DVU2149 at 2248.564 kb on + strand, within DVU2149 at 2248.571 kb on + strand, within DVU2149 at 2248.572 kb on - strand, within DVU2149 at 2248.572 kb on - strand, within DVU2149 at 2248.576 kb on + strand, within DVU2149 at 2248.579 kb on - strand, within DVU2149 at 2248.579 kb on - strand, within DVU2149 at 2248.584 kb on - strand, within DVU2149 at 2248.588 kb on + strand, within DVU2149 at 2248.595 kb on + strand, within DVU2149 at 2248.596 kb on - strand, within DVU2149 at 2248.718 kb on - strand, within DVU2149 at 2248.722 kb on + strand, within DVU2149 at 2248.793 kb on + strand, within DVU2149 at 2248.830 kb on - strand, within DVU2149 at 2248.843 kb on + strand, within DVU2149 at 2248.928 kb on - strand, within DVU2149 at 2248.968 kb on - strand, within DVU2149 at 2249.010 kb on + strand, within DVU2149 at 2249.037 kb on - strand, within DVU2149 at 2249.092 kb on + strand at 2249.154 kb on + strand at 2249.216 kb on + strand at 2249.282 kb on - strand at 2249.347 kb on - strand, within dksA at 2249.454 kb on + strand, within dksA at 2249.550 kb on + strand, within dksA at 2249.558 kb on - strand, within dksA at 2249.606 kb on - strand at 2249.863 kb on + strand at 2249.871 kb on - strand at 2249.871 kb on - strand at 2249.898 kb on + strand at 2249.903 kb on + strand at 2250.167 kb on + strand at 2250.188 kb on + strand at 2250.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 2,248,277 - DVU2149 0.44 +0.6 2,248,328 - DVU2149 0.47 +0.3 2,248,390 - DVU2149 0.50 +0.0 2,248,422 + DVU2149 0.52 -0.9 2,248,473 + DVU2149 0.55 +0.2 2,248,473 + DVU2149 0.55 +0.2 2,248,484 + DVU2149 0.56 -1.0 2,248,564 + DVU2149 0.60 +0.1 2,248,571 + DVU2149 0.61 -0.5 2,248,572 - DVU2149 0.61 -0.2 2,248,572 - DVU2149 0.61 -0.3 2,248,576 + DVU2149 0.61 -0.4 2,248,579 - DVU2149 0.61 +1.1 2,248,579 - DVU2149 0.61 -0.7 2,248,584 - DVU2149 0.61 +0.5 2,248,588 + DVU2149 0.61 -0.3 2,248,595 + DVU2149 0.62 -0.2 2,248,596 - DVU2149 0.62 -0.5 2,248,718 - DVU2149 0.69 -0.2 2,248,722 + DVU2149 0.69 +0.0 2,248,793 + DVU2149 0.73 +0.4 2,248,830 - DVU2149 0.75 +0.3 2,248,843 + DVU2149 0.76 +0.1 2,248,928 - DVU2149 0.81 -0.6 2,248,968 - DVU2149 0.83 +0.3 2,249,010 + DVU2149 0.86 +0.0 2,249,037 - DVU2149 0.87 -0.2 2,249,092 + -0.0 2,249,154 + -0.1 2,249,216 + +0.3 2,249,282 - -0.4 2,249,347 - dksA DVU2150 0.19 -1.5 2,249,454 + dksA DVU2150 0.49 -1.1 2,249,550 + dksA DVU2150 0.75 -1.8 2,249,558 - dksA DVU2150 0.77 +0.4 2,249,606 - -0.7 2,249,863 + +0.4 2,249,871 - -0.5 2,249,871 - -1.8 2,249,898 + -1.0 2,249,903 + -0.1 2,250,167 + -0.8 2,250,188 + -0.0 2,250,234 - -0.2
Or see this region's nucleotide sequence