Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2049 and DVU2051 are separated by 791 nucleotides DVU2051 and tRNA-Met are separated by 220 nucleotides tRNA-Met and dmt are separated by 102 nucleotides
DVU2049: DVU2049 - transposase OrfA, IS3 family, degenerate (TIGR), at 2,126,385 to 2,126,851
DVU2049
DVU2051: DVU2051 - hypothetical protein (TIGR), at 2,127,643 to 2,128,941
DVU2051
DVU_tRNA-Met-4: tRNA-Met - tRNA with anticodon CAT for Met (VIMSS-AUTO), at 2,129,162 to 2,129,238
tRNA-Met
DVU2052: dmt - glycosyl transferase, group 2 family protein (TIGR), at 2,129,341 to 2,130,063
dmt
Position (kb)
2127
2128
2129 Strain fitness (log2 ratio)
-1
0
1 at 2126.645 kb on + strand, within DVU2049 at 2126.683 kb on - strand, within DVU2049 at 2126.691 kb on + strand, within DVU2049 at 2126.698 kb on + strand, within DVU2049 at 2126.922 kb on - strand at 2126.965 kb on + strand at 2126.973 kb on - strand at 2127.121 kb on + strand at 2127.140 kb on + strand at 2127.140 kb on + strand at 2127.148 kb on - strand at 2127.150 kb on + strand at 2127.152 kb on + strand at 2127.199 kb on + strand at 2127.200 kb on + strand at 2127.200 kb on + strand at 2127.208 kb on - strand at 2127.208 kb on - strand at 2127.221 kb on + strand at 2127.410 kb on - strand at 2127.555 kb on - strand at 2128.493 kb on + strand, within DVU2051 at 2128.500 kb on + strand, within DVU2051 at 2128.739 kb on + strand, within DVU2051 at 2128.824 kb on - strand at 2129.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 2,126,645 + DVU2049 0.56 -0.5 2,126,683 - DVU2049 0.64 +0.0 2,126,691 + DVU2049 0.66 -0.5 2,126,698 + DVU2049 0.67 +0.1 2,126,922 - -0.2 2,126,965 + +0.4 2,126,973 - -0.5 2,127,121 + -0.2 2,127,140 + +0.2 2,127,140 + +0.8 2,127,148 - -0.3 2,127,150 + +0.3 2,127,152 + +0.9 2,127,199 + +0.1 2,127,200 + -0.5 2,127,200 + -0.2 2,127,208 - +0.5 2,127,208 - -0.2 2,127,221 + -1.3 2,127,410 - +0.2 2,127,555 - -0.4 2,128,493 + DVU2051 0.65 +0.1 2,128,500 + DVU2051 0.66 +0.1 2,128,739 + DVU2051 0.84 +0.3 2,128,824 - +0.5 2,129,077 + -0.6
Or see this region's nucleotide sequence