Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2013 and fprA-1 are separated by 74 nucleotides fprA-1 and DORF15009 overlap by 4 nucleotides DORF15009 and DVU2016 overlap by 115 nucleotides
DVU2013: DVU2013 - hybrid cluster protein (TIGR), at 2,090,668 to 2,092,329
DVU2013
DVU2014: fprA-1 - metallo-beta-lactamase family protein (TIGR), at 2,092,404 to 2,093,579
fprA-1
DORF15009: DORF15009 - SEED: Response regulator containing a CheY-like receiver domain and a GGDEF domain, at 2,093,576 to 2,093,878
DORF15009
DVU2016: DVU2016 - GGDEF domain protein (TIGR), at 2,093,764 to 2,094,645
DVU2016
Position (kb)
2092
2093
2094 Strain fitness (log2 ratio)
-2
-1
0
1 at 2091.411 kb on + strand, within DVU2013 at 2091.536 kb on + strand, within DVU2013 at 2091.544 kb on - strand, within DVU2013 at 2091.602 kb on - strand, within DVU2013 at 2091.672 kb on - strand, within DVU2013 at 2091.714 kb on - strand, within DVU2013 at 2091.840 kb on + strand, within DVU2013 at 2091.880 kb on - strand, within DVU2013 at 2091.895 kb on + strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.940 kb on - strand, within DVU2013 at 2091.961 kb on - strand, within DVU2013 at 2092.120 kb on - strand, within DVU2013 at 2092.162 kb on - strand, within DVU2013 at 2092.274 kb on + strand at 2092.379 kb on + strand at 2092.458 kb on + strand at 2092.491 kb on + strand at 2092.500 kb on - strand at 2092.500 kb on - strand at 2092.500 kb on - strand at 2092.502 kb on + strand at 2092.502 kb on + strand at 2092.517 kb on - strand at 2092.648 kb on + strand, within fprA-1 at 2092.665 kb on + strand, within fprA-1 at 2092.812 kb on - strand, within fprA-1 at 2092.862 kb on - strand, within fprA-1 at 2092.911 kb on + strand, within fprA-1 at 2093.082 kb on + strand, within fprA-1 at 2093.167 kb on - strand, within fprA-1 at 2093.256 kb on + strand, within fprA-1 at 2093.272 kb on - strand, within fprA-1 at 2093.286 kb on + strand, within fprA-1 at 2093.313 kb on - strand, within fprA-1 at 2093.399 kb on - strand, within fprA-1 at 2093.424 kb on - strand, within fprA-1 at 2093.504 kb on + strand at 2094.049 kb on + strand, within DVU2016 at 2094.391 kb on + strand, within DVU2016 at 2094.391 kb on + strand, within DVU2016
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 2,091,411 + DVU2013 0.45 -0.5 2,091,536 + DVU2013 0.52 +0.8 2,091,544 - DVU2013 0.53 +0.1 2,091,602 - DVU2013 0.56 +0.0 2,091,672 - DVU2013 0.60 -0.4 2,091,714 - DVU2013 0.63 +0.3 2,091,840 + DVU2013 0.71 +0.1 2,091,880 - DVU2013 0.73 +0.9 2,091,895 + DVU2013 0.74 +0.3 2,091,940 - DVU2013 0.77 +0.2 2,091,940 - DVU2013 0.77 -1.3 2,091,940 - DVU2013 0.77 -0.3 2,091,940 - DVU2013 0.77 +0.0 2,091,961 - DVU2013 0.78 +0.2 2,092,120 - DVU2013 0.87 +0.0 2,092,162 - DVU2013 0.90 -0.0 2,092,274 + -0.2 2,092,379 + -0.1 2,092,458 + +0.2 2,092,491 + -0.6 2,092,500 - -0.3 2,092,500 - +0.2 2,092,500 - -0.1 2,092,502 + -0.4 2,092,502 + -1.9 2,092,517 - -1.0 2,092,648 + fprA-1 DVU2014 0.21 +0.1 2,092,665 + fprA-1 DVU2014 0.22 -0.8 2,092,812 - fprA-1 DVU2014 0.35 +0.7 2,092,862 - fprA-1 DVU2014 0.39 +1.1 2,092,911 + fprA-1 DVU2014 0.43 -0.0 2,093,082 + fprA-1 DVU2014 0.58 -0.4 2,093,167 - fprA-1 DVU2014 0.65 +0.2 2,093,256 + fprA-1 DVU2014 0.72 +0.8 2,093,272 - fprA-1 DVU2014 0.74 -0.3 2,093,286 + fprA-1 DVU2014 0.75 +0.3 2,093,313 - fprA-1 DVU2014 0.77 -1.3 2,093,399 - fprA-1 DVU2014 0.85 +1.7 2,093,424 - fprA-1 DVU2014 0.87 -0.7 2,093,504 + +0.1 2,094,049 + DVU2016 0.32 -0.7 2,094,391 + DVU2016 0.71 -0.2 2,094,391 + DVU2016 0.71 -0.4
Or see this region's nucleotide sequence