Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1371 and DVU1372 are separated by 104 nucleotides DVU1372 and divIVA are separated by 8 nucleotides divIVA and DVU1374 overlap by 8 nucleotides DVU1374 and DVU1375 are separated by 20 nucleotides DVU1375 and ilvB-2 are separated by 0 nucleotides
DVU1371: DVU1371 - HAD-superfamily hydrolase, subfamily IA (TIGR), at 1,449,380 to 1,450,054
DVU1371
DVU1372: DVU1372 - membrane protein, putative (TIGR), at 1,450,159 to 1,450,461
DVU1372
DVU1373: divIVA - cell-division initiation protein DivIVA (TIGR), at 1,450,470 to 1,450,979
divIVA
DVU1374: DVU1374 - conserved hypothetical protein (TIGR), at 1,450,972 to 1,451,292
DVU1374
DVU1375: DVU1375 - hypothetical protein (TIGR), at 1,451,313 to 1,451,543
DVU1375
DVU1376: ilvB-2 - acetolactate synthase, large subunit, biosynthetic type (TIGR), at 1,451,544 to 1,453,235
ilvB-2
Position (kb)
1450
1451 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1449.606 kb on - strand, within DVU1371 at 1449.677 kb on - strand, within DVU1371 at 1449.751 kb on - strand, within DVU1371 at 1449.752 kb on - strand, within DVU1371 at 1449.763 kb on - strand, within DVU1371 at 1449.772 kb on - strand, within DVU1371 at 1449.773 kb on - strand, within DVU1371 at 1449.854 kb on - strand, within DVU1371 at 1449.949 kb on + strand, within DVU1371 at 1449.966 kb on + strand, within DVU1371 at 1450.008 kb on - strand at 1450.161 kb on - strand at 1450.249 kb on + strand, within DVU1372 at 1450.254 kb on + strand, within DVU1372 at 1450.254 kb on + strand, within DVU1372 at 1450.268 kb on - strand, within DVU1372 at 1450.275 kb on + strand, within DVU1372 at 1450.283 kb on - strand, within DVU1372 at 1450.297 kb on - strand, within DVU1372 at 1450.353 kb on - strand, within DVU1372 at 1450.364 kb on + strand, within DVU1372 at 1450.442 kb on - strand at 1450.492 kb on + strand at 1450.687 kb on - strand, within divIVA at 1450.689 kb on + strand, within divIVA at 1450.992 kb on + strand at 1451.000 kb on - strand at 1451.060 kb on + strand, within DVU1374 at 1451.084 kb on + strand, within DVU1374 at 1451.097 kb on + strand, within DVU1374 at 1451.126 kb on - strand, within DVU1374 at 1451.172 kb on + strand, within DVU1374 at 1451.262 kb on + strand at 1451.352 kb on + strand, within DVU1375 at 1451.352 kb on + strand, within DVU1375 at 1451.360 kb on - strand, within DVU1375 at 1451.362 kb on - strand, within DVU1375 at 1451.522 kb on + strand at 1451.530 kb on - strand at 1451.530 kb on - strand at 1451.537 kb on + strand at 1451.567 kb on - strand at 1451.723 kb on + strand, within ilvB-2 at 1451.730 kb on + strand, within ilvB-2 at 1451.738 kb on - strand, within ilvB-2 at 1451.740 kb on + strand, within ilvB-2 at 1451.813 kb on - strand, within ilvB-2 at 1451.831 kb on + strand, within ilvB-2 at 1451.924 kb on + strand, within ilvB-2 at 1451.924 kb on + strand, within ilvB-2 at 1451.932 kb on - strand, within ilvB-2 at 1451.932 kb on - strand, within ilvB-2
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 1,449,606 - DVU1371 0.33 -0.1 1,449,677 - DVU1371 0.44 -2.1 1,449,751 - DVU1371 0.55 -0.1 1,449,752 - DVU1371 0.55 +0.3 1,449,763 - DVU1371 0.57 -1.2 1,449,772 - DVU1371 0.58 +0.4 1,449,773 - DVU1371 0.58 +0.1 1,449,854 - DVU1371 0.70 +0.1 1,449,949 + DVU1371 0.84 +0.6 1,449,966 + DVU1371 0.87 -0.7 1,450,008 - -0.0 1,450,161 - +0.1 1,450,249 + DVU1372 0.30 -0.1 1,450,254 + DVU1372 0.31 -0.4 1,450,254 + DVU1372 0.31 -0.5 1,450,268 - DVU1372 0.36 -0.3 1,450,275 + DVU1372 0.38 -0.4 1,450,283 - DVU1372 0.41 -1.5 1,450,297 - DVU1372 0.46 +0.2 1,450,353 - DVU1372 0.64 -1.2 1,450,364 + DVU1372 0.68 -0.0 1,450,442 - -0.7 1,450,492 + -0.3 1,450,687 - divIVA DVU1373 0.43 -1.3 1,450,689 + divIVA DVU1373 0.43 -0.6 1,450,992 + -0.2 1,451,000 - -0.1 1,451,060 + DVU1374 0.27 -0.5 1,451,084 + DVU1374 0.35 -0.2 1,451,097 + DVU1374 0.39 -0.4 1,451,126 - DVU1374 0.48 -0.3 1,451,172 + DVU1374 0.62 -0.6 1,451,262 + -1.2 1,451,352 + DVU1375 0.17 +2.5 1,451,352 + DVU1375 0.17 -0.4 1,451,360 - DVU1375 0.20 -0.1 1,451,362 - DVU1375 0.21 +0.2 1,451,522 + -1.3 1,451,530 - -1.0 1,451,530 - -0.2 1,451,537 + -0.2 1,451,567 - -0.4 1,451,723 + ilvB-2 DVU1376 0.11 +0.9 1,451,730 + ilvB-2 DVU1376 0.11 -0.0 1,451,738 - ilvB-2 DVU1376 0.11 -0.1 1,451,740 + ilvB-2 DVU1376 0.12 +0.5 1,451,813 - ilvB-2 DVU1376 0.16 -1.3 1,451,831 + ilvB-2 DVU1376 0.17 -0.6 1,451,924 + ilvB-2 DVU1376 0.22 -0.1 1,451,924 + ilvB-2 DVU1376 0.22 +0.1 1,451,932 - ilvB-2 DVU1376 0.23 +0.6 1,451,932 - ilvB-2 DVU1376 0.23 +0.1
Or see this region's nucleotide sequence