Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1266 and DVU1267 are separated by 10 nucleotides DVU1267 and DVU1268 overlap by 4 nucleotides DVU1268 and DORF10279 are separated by 152 nucleotides DORF10279 and pilT-1 are separated by 31 nucleotides
DVU1266: DVU1266 - hypothetical protein (TIGR), at 1,354,391 to 1,357,828
DVU1266
DVU1267: DVU1267 - hypothetical protein (TIGR), at 1,357,839 to 1,358,522
DVU1267
DVU1268: DVU1268 - hypothetical protein (TIGR), at 1,358,519 to 1,358,932
DVU1268
DORF10279: DORF10279 - no description, at 1,359,085 to 1,359,201
DORF10279
DVU1270: pilT-1 - twitching motility protein (TIGR), at 1,359,233 to 1,360,513
pilT-1
Position (kb)
1358
1359 Strain fitness (log2 ratio)
-1
0
1 at 1357.578 kb on - strand at 1357.585 kb on + strand at 1357.600 kb on + strand at 1357.600 kb on + strand at 1357.622 kb on - strand at 1357.721 kb on - strand at 1357.823 kb on - strand at 1357.823 kb on - strand at 1357.859 kb on - strand at 1357.895 kb on + strand at 1358.017 kb on + strand, within DVU1267 at 1358.242 kb on - strand, within DVU1267 at 1358.242 kb on - strand, within DVU1267 at 1358.242 kb on - strand, within DVU1267 at 1358.345 kb on - strand, within DVU1267 at 1358.354 kb on + strand, within DVU1267 at 1358.409 kb on - strand, within DVU1267 at 1358.430 kb on - strand, within DVU1267 at 1358.491 kb on - strand at 1358.496 kb on - strand at 1358.530 kb on + strand at 1358.591 kb on + strand, within DVU1268 at 1358.655 kb on - strand, within DVU1268 at 1358.683 kb on - strand, within DVU1268 at 1358.688 kb on + strand, within DVU1268 at 1358.729 kb on + strand, within DVU1268 at 1358.738 kb on + strand, within DVU1268 at 1358.756 kb on + strand, within DVU1268 at 1358.803 kb on + strand, within DVU1268 at 1359.099 kb on + strand, within DORF10279 at 1359.107 kb on - strand, within DORF10279 at 1359.107 kb on - strand, within DORF10279 at 1359.107 kb on - strand, within DORF10279 at 1359.107 kb on - strand, within DORF10279 at 1359.107 kb on - strand, within DORF10279 at 1359.172 kb on - strand, within DORF10279 at 1359.196 kb on + strand at 1359.196 kb on + strand at 1359.204 kb on - strand at 1359.307 kb on + strand at 1359.328 kb on + strand at 1359.339 kb on + strand at 1359.438 kb on - strand, within pilT-1 at 1359.481 kb on + strand, within pilT-1 at 1359.662 kb on + strand, within pilT-1 at 1359.761 kb on + strand, within pilT-1 at 1359.776 kb on + strand, within pilT-1 at 1359.791 kb on - strand, within pilT-1 at 1359.791 kb on - strand, within pilT-1 at 1359.802 kb on + strand, within pilT-1 at 1359.837 kb on + strand, within pilT-1 at 1359.868 kb on + strand, within pilT-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 1,357,578 - +0.4 1,357,585 + +0.7 1,357,600 + -1.3 1,357,600 + -0.4 1,357,622 - +0.2 1,357,721 - -0.7 1,357,823 - -0.4 1,357,823 - -0.2 1,357,859 - -0.8 1,357,895 + -0.6 1,358,017 + DVU1267 0.26 +0.8 1,358,242 - DVU1267 0.59 +0.6 1,358,242 - DVU1267 0.59 +0.6 1,358,242 - DVU1267 0.59 +0.0 1,358,345 - DVU1267 0.74 +0.1 1,358,354 + DVU1267 0.75 -0.2 1,358,409 - DVU1267 0.83 -0.2 1,358,430 - DVU1267 0.86 +0.8 1,358,491 - +0.2 1,358,496 - -0.3 1,358,530 + -0.1 1,358,591 + DVU1268 0.17 +0.7 1,358,655 - DVU1268 0.33 -0.0 1,358,683 - DVU1268 0.40 +1.3 1,358,688 + DVU1268 0.41 +1.0 1,358,729 + DVU1268 0.51 -0.4 1,358,738 + DVU1268 0.53 +0.4 1,358,756 + DVU1268 0.57 -0.0 1,358,803 + DVU1268 0.69 +0.2 1,359,099 + DORF10279 0.12 -0.9 1,359,107 - DORF10279 0.19 -1.7 1,359,107 - DORF10279 0.19 -0.3 1,359,107 - DORF10279 0.19 -0.0 1,359,107 - DORF10279 0.19 -0.8 1,359,107 - DORF10279 0.19 +1.4 1,359,172 - DORF10279 0.74 -0.2 1,359,196 + +0.2 1,359,196 + -0.3 1,359,204 - -0.1 1,359,307 + -0.0 1,359,328 + -1.0 1,359,339 + -0.1 1,359,438 - pilT-1 DVU1270 0.16 -1.3 1,359,481 + pilT-1 DVU1270 0.19 +0.3 1,359,662 + pilT-1 DVU1270 0.33 +0.2 1,359,761 + pilT-1 DVU1270 0.41 -1.4 1,359,776 + pilT-1 DVU1270 0.42 -1.0 1,359,791 - pilT-1 DVU1270 0.44 +0.0 1,359,791 - pilT-1 DVU1270 0.44 -0.4 1,359,802 + pilT-1 DVU1270 0.44 -0.3 1,359,837 + pilT-1 DVU1270 0.47 +0.1 1,359,868 + pilT-1 DVU1270 0.50 -0.2
Or see this region's nucleotide sequence