Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1252 and DVU1253 are separated by 50 nucleotides DVU1253 and DVU1254 are separated by 38 nucleotides DVU1254 and DVU1255 are separated by 352 nucleotides
DVU1252: DVU1252 - membrane protein, putative (TIGR), at 1,337,944 to 1,340,220
DVU1252
DVU1253: DVU1253 - hypothetical protein (TIGR), at 1,340,271 to 1,340,399
DVU1253
DVU1254: DVU1254 - conserved hypothetical protein (TIGR), at 1,340,438 to 1,340,908
DVU1254
DVU1255: DVU1255 - Sua5/YciO/YrdC/YwlC family protein (TIGR), at 1,341,261 to 1,341,893
DVU1255
Position (kb)
1340
1341 Strain fitness (log2 ratio)
-2
-1
0
1 at 1340.218 kb on - strand at 1340.280 kb on - strand at 1340.295 kb on + strand, within DVU1253 at 1340.337 kb on - strand, within DVU1253 at 1340.342 kb on + strand, within DVU1253 at 1340.443 kb on + strand at 1340.467 kb on + strand at 1340.467 kb on + strand at 1340.483 kb on + strand at 1340.486 kb on - strand, within DVU1254 at 1340.574 kb on + strand, within DVU1254 at 1340.584 kb on + strand, within DVU1254 at 1340.689 kb on + strand, within DVU1254 at 1340.817 kb on - strand, within DVU1254 at 1340.817 kb on - strand, within DVU1254 at 1340.829 kb on - strand, within DVU1254 at 1340.840 kb on - strand, within DVU1254 at 1340.842 kb on - strand, within DVU1254 at 1340.901 kb on + strand at 1340.903 kb on + strand at 1340.909 kb on - strand at 1340.909 kb on - strand at 1340.911 kb on - strand at 1340.956 kb on - strand at 1340.966 kb on - strand at 1340.966 kb on - strand at 1341.012 kb on - strand at 1341.093 kb on + strand at 1341.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 1,340,218 - -2.0 1,340,280 - +0.1 1,340,295 + DVU1253 0.19 +0.2 1,340,337 - DVU1253 0.51 +0.1 1,340,342 + DVU1253 0.55 -0.4 1,340,443 + -0.4 1,340,467 + -0.4 1,340,467 + -1.0 1,340,483 + -0.0 1,340,486 - DVU1254 0.10 -0.0 1,340,574 + DVU1254 0.29 +0.5 1,340,584 + DVU1254 0.31 -0.2 1,340,689 + DVU1254 0.53 +0.2 1,340,817 - DVU1254 0.80 -0.1 1,340,817 - DVU1254 0.80 -0.2 1,340,829 - DVU1254 0.83 +0.0 1,340,840 - DVU1254 0.85 -0.8 1,340,842 - DVU1254 0.86 -0.3 1,340,901 + -0.1 1,340,903 + -0.4 1,340,909 - -0.2 1,340,909 - +0.1 1,340,911 - +0.2 1,340,956 - -1.5 1,340,966 - +0.3 1,340,966 - -1.1 1,341,012 - -0.2 1,341,093 + +0.3 1,341,166 - +0.1
Or see this region's nucleotide sequence