Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1239 and DVU1240 overlap by 11 nucleotides DVU1240 and DVU1241 are separated by 305 nucleotides DVU1241 and vacJhomolog are separated by 122 nucleotides vacJhomolog and DVU1243 are separated by 8 nucleotides
DVU1239: DVU1239 - membrane protein, putative (TIGR), at 1,327,711 to 1,328,313
DVU1239
DVU1240: DVU1240 - hypothetical protein (TIGR), at 1,328,303 to 1,328,710
DVU1240
DVU1241: DVU1241 - conserved hypothetical protein (TIGR), at 1,329,016 to 1,329,333
DVU1241
DVU1242: vacJhomolog - vacJ lipoprotein, putative (TIGR), at 1,329,456 to 1,330,277
vacJhomolog
DVU1243: DVU1243 - conserved hypothetical protein (TIGR), at 1,330,286 to 1,330,918
DVU1243
Position (kb)
1329
1330 Strain fitness (log2 ratio)
-2
-1
0
1 at 1328.018 kb on - strand, within DVU1239 at 1328.055 kb on + strand, within DVU1239 at 1328.168 kb on + strand, within DVU1239 at 1328.169 kb on - strand, within DVU1239 at 1328.220 kb on + strand, within DVU1239 at 1328.300 kb on - strand at 1328.342 kb on - strand at 1328.344 kb on + strand, within DVU1240 at 1328.349 kb on - strand, within DVU1240 at 1328.352 kb on + strand, within DVU1240 at 1328.388 kb on - strand, within DVU1240 at 1328.441 kb on - strand, within DVU1240 at 1328.489 kb on + strand, within DVU1240 at 1328.499 kb on - strand, within DVU1240 at 1328.504 kb on - strand, within DVU1240 at 1328.554 kb on - strand, within DVU1240 at 1328.587 kb on - strand, within DVU1240 at 1328.633 kb on + strand, within DVU1240 at 1328.633 kb on + strand, within DVU1240 at 1328.633 kb on + strand, within DVU1240 at 1328.633 kb on + strand, within DVU1240 at 1328.641 kb on - strand, within DVU1240 at 1328.657 kb on - strand, within DVU1240 at 1328.678 kb on + strand at 1328.678 kb on + strand at 1328.727 kb on - strand at 1328.732 kb on - strand at 1328.737 kb on - strand at 1328.789 kb on + strand at 1328.791 kb on - strand at 1328.797 kb on - strand at 1328.891 kb on - strand at 1328.902 kb on + strand at 1328.902 kb on + strand at 1329.035 kb on - strand at 1329.073 kb on + strand, within DVU1241 at 1329.135 kb on - strand, within DVU1241 at 1329.156 kb on + strand, within DVU1241 at 1329.226 kb on - strand, within DVU1241 at 1329.289 kb on + strand, within DVU1241 at 1329.290 kb on + strand, within DVU1241 at 1329.290 kb on + strand, within DVU1241 at 1329.320 kb on - strand at 1329.357 kb on - strand at 1329.739 kb on - strand, within vacJhomolog at 1330.202 kb on - strand at 1330.224 kb on + strand at 1330.231 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 1,328,018 - DVU1239 0.51 -0.6 1,328,055 + DVU1239 0.57 -0.4 1,328,168 + DVU1239 0.76 +0.9 1,328,169 - DVU1239 0.76 -0.3 1,328,220 + DVU1239 0.84 +0.2 1,328,300 - -0.3 1,328,342 - -1.3 1,328,344 + DVU1240 0.10 -0.7 1,328,349 - DVU1240 0.11 -2.2 1,328,352 + DVU1240 0.12 -0.6 1,328,388 - DVU1240 0.21 +1.5 1,328,441 - DVU1240 0.34 -0.6 1,328,489 + DVU1240 0.46 -1.1 1,328,499 - DVU1240 0.48 -0.2 1,328,504 - DVU1240 0.49 -1.6 1,328,554 - DVU1240 0.62 -0.9 1,328,587 - DVU1240 0.70 +0.3 1,328,633 + DVU1240 0.81 -0.2 1,328,633 + DVU1240 0.81 -1.3 1,328,633 + DVU1240 0.81 +0.1 1,328,633 + DVU1240 0.81 -0.6 1,328,641 - DVU1240 0.83 -0.2 1,328,657 - DVU1240 0.87 -0.2 1,328,678 + +0.0 1,328,678 + +0.7 1,328,727 - -0.1 1,328,732 - +0.1 1,328,737 - -0.7 1,328,789 + -0.0 1,328,791 - +0.1 1,328,797 - +0.1 1,328,891 - -0.4 1,328,902 + -0.5 1,328,902 + -1.6 1,329,035 - +0.6 1,329,073 + DVU1241 0.18 -1.4 1,329,135 - DVU1241 0.37 -0.7 1,329,156 + DVU1241 0.44 -1.2 1,329,226 - DVU1241 0.66 -0.9 1,329,289 + DVU1241 0.86 -0.7 1,329,290 + DVU1241 0.86 -0.2 1,329,290 + DVU1241 0.86 -0.2 1,329,320 - +0.7 1,329,357 - -0.2 1,329,739 - vacJhomolog DVU1242 0.34 -0.1 1,330,202 - -0.9 1,330,224 + -0.3 1,330,231 + +0.9
Or see this region's nucleotide sequence