Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phoU and DVU1086 are separated by 427 nucleotides DVU1086 and DVU1087 are separated by 3 nucleotides DVU1087 and DVU1088 are separated by 46 nucleotides DVU1088 and alaS are separated by 379 nucleotides
DVU1085: phoU - phosphate transport system protein PhoU (TIGR), at 1,188,620 to 1,189,285
phoU
DVU1086: DVU1086 - hypothetical protein (TIGR), at 1,189,713 to 1,190,192
DVU1086
DVU1087: DVU1087 - conserved hypothetical protein (TIGR), at 1,190,196 to 1,191,176
DVU1087
DVU1088: DVU1088 - hypothetical protein (TIGR), at 1,191,223 to 1,191,399
DVU1088
DVU1089: alaS - alanyl-tRNA synthetase (TIGR), at 1,191,779 to 1,194,418
alaS
Position (kb)
1190
1191
1192 Strain fitness (log2 ratio)
-2
-1
0
1 at 1189.280 kb on + strand at 1189.336 kb on - strand at 1189.390 kb on - strand at 1189.514 kb on - strand at 1189.627 kb on + strand at 1189.627 kb on + strand at 1189.653 kb on + strand at 1189.789 kb on + strand, within DVU1086 at 1189.848 kb on + strand, within DVU1086 at 1189.911 kb on + strand, within DVU1086 at 1189.963 kb on + strand, within DVU1086 at 1189.966 kb on - strand, within DVU1086 at 1190.011 kb on - strand, within DVU1086 at 1190.063 kb on - strand, within DVU1086 at 1190.084 kb on + strand, within DVU1086 at 1190.084 kb on + strand, within DVU1086 at 1190.106 kb on + strand, within DVU1086 at 1190.186 kb on + strand at 1190.215 kb on - strand at 1190.537 kb on - strand, within DVU1087 at 1190.640 kb on + strand, within DVU1087 at 1190.768 kb on - strand, within DVU1087 at 1190.773 kb on - strand, within DVU1087 at 1190.915 kb on + strand, within DVU1087 at 1190.938 kb on - strand, within DVU1087 at 1190.985 kb on - strand, within DVU1087 at 1191.028 kb on - strand, within DVU1087 at 1191.083 kb on + strand at 1191.083 kb on + strand at 1191.109 kb on + strand at 1191.112 kb on - strand at 1191.117 kb on - strand at 1191.117 kb on - strand at 1191.284 kb on + strand, within DVU1088 at 1191.402 kb on - strand at 1191.402 kb on - strand at 1191.402 kb on - strand at 1191.427 kb on + strand at 1191.435 kb on - strand at 1191.446 kb on - strand at 1191.472 kb on + strand at 1191.480 kb on - strand at 1191.514 kb on - strand at 1191.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 1,189,280 + -1.0 1,189,336 - -0.3 1,189,390 - -0.1 1,189,514 - -0.4 1,189,627 + -0.8 1,189,627 + -1.8 1,189,653 + -0.2 1,189,789 + DVU1086 0.16 -0.8 1,189,848 + DVU1086 0.28 -0.4 1,189,911 + DVU1086 0.41 -1.1 1,189,963 + DVU1086 0.52 -0.5 1,189,966 - DVU1086 0.53 -0.4 1,190,011 - DVU1086 0.62 +0.3 1,190,063 - DVU1086 0.73 -1.3 1,190,084 + DVU1086 0.77 -0.3 1,190,084 + DVU1086 0.77 -0.3 1,190,106 + DVU1086 0.82 +0.0 1,190,186 + -1.0 1,190,215 - -0.3 1,190,537 - DVU1087 0.35 -0.6 1,190,640 + DVU1087 0.45 -1.0 1,190,768 - DVU1087 0.58 -0.3 1,190,773 - DVU1087 0.59 -0.9 1,190,915 + DVU1087 0.73 -0.8 1,190,938 - DVU1087 0.76 -0.9 1,190,985 - DVU1087 0.80 -0.8 1,191,028 - DVU1087 0.85 -0.8 1,191,083 + -0.1 1,191,083 + -1.8 1,191,109 + -0.6 1,191,112 - -2.1 1,191,117 - -0.1 1,191,117 - -0.8 1,191,284 + DVU1088 0.34 -1.2 1,191,402 - -2.3 1,191,402 - -0.1 1,191,402 - -0.5 1,191,427 + -0.3 1,191,435 - +0.0 1,191,446 - -0.7 1,191,472 + +0.4 1,191,480 - -0.0 1,191,514 - -1.0 1,191,665 + -1.5
Or see this region's nucleotide sequence