Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbA and rfbC overlap by 4 nucleotides rfbC and DVU0699 are separated by 176 nucleotides
DVU0697: rfbA - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (TIGR), at 771,808 to 773,226
rfbA
DVU0698: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase (TIGR), at 773,223 to 773,795
rfbC
DVU0699: DVU0699 - sensory box sensor histidine kinase/response regulator, authentic frameshift (TIGR), at 773,972 to 775,650
DVU0699
Position (kb)
773
774 Strain fitness (log2 ratio)
-1
0
1 at 773.386 kb on - strand, within rfbC at 773.395 kb on + strand, within rfbC at 773.482 kb on - strand, within rfbC at 773.497 kb on - strand, within rfbC at 773.542 kb on - strand, within rfbC at 773.572 kb on - strand, within rfbC at 773.577 kb on - strand, within rfbC at 773.878 kb on - strand at 773.882 kb on + strand at 773.981 kb on + strand at 773.983 kb on + strand at 774.054 kb on - strand at 774.054 kb on - strand at 774.060 kb on - strand at 774.068 kb on + strand at 774.123 kb on + strand at 774.123 kb on + strand at 774.131 kb on - strand at 774.131 kb on - strand at 774.135 kb on + strand at 774.151 kb on + strand, within DVU0699 at 774.158 kb on + strand, within DVU0699 at 774.208 kb on + strand, within DVU0699 at 774.286 kb on + strand, within DVU0699 at 774.323 kb on + strand, within DVU0699 at 774.375 kb on - strand, within DVU0699 at 774.460 kb on + strand, within DVU0699 at 774.540 kb on + strand, within DVU0699 at 774.541 kb on - strand, within DVU0699 at 774.564 kb on + strand, within DVU0699 at 774.567 kb on - strand, within DVU0699 at 774.567 kb on - strand, within DVU0699 at 774.593 kb on + strand, within DVU0699 at 774.685 kb on + strand, within DVU0699 at 774.706 kb on + strand, within DVU0699 at 774.706 kb on + strand, within DVU0699 at 774.716 kb on - strand, within DVU0699
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 773,386 - rfbC DVU0698 0.28 +0.4 773,395 + rfbC DVU0698 0.30 -0.0 773,482 - rfbC DVU0698 0.45 +0.3 773,497 - rfbC DVU0698 0.48 -0.1 773,542 - rfbC DVU0698 0.56 -0.3 773,572 - rfbC DVU0698 0.61 +0.2 773,577 - rfbC DVU0698 0.62 -0.2 773,878 - -0.2 773,882 + +0.3 773,981 + +0.0 773,983 + -0.8 774,054 - +0.5 774,054 - -0.2 774,060 - -0.4 774,068 + -0.1 774,123 + -0.2 774,123 + +0.1 774,131 - -0.5 774,131 - -0.3 774,135 + -0.4 774,151 + DVU0699 0.11 -0.5 774,158 + DVU0699 0.11 -0.2 774,208 + DVU0699 0.14 -0.4 774,286 + DVU0699 0.19 -0.0 774,323 + DVU0699 0.21 -0.4 774,375 - DVU0699 0.24 +0.2 774,460 + DVU0699 0.29 +0.1 774,540 + DVU0699 0.34 -0.0 774,541 - DVU0699 0.34 -0.2 774,564 + DVU0699 0.35 -0.7 774,567 - DVU0699 0.35 -0.1 774,567 - DVU0699 0.35 +0.1 774,593 + DVU0699 0.37 +0.1 774,685 + DVU0699 0.42 -0.0 774,706 + DVU0699 0.44 -0.2 774,706 + DVU0699 0.44 -0.2 774,716 - DVU0699 0.44 +0.9
Or see this region's nucleotide sequence