Experiment: MoLS4 without vitamins with Casaminos 0.2%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0659 and DVU0660 are separated by 210 nucleotides DVU0660 and DVU0661 are separated by 217 nucleotides DVU0661 and cysE are separated by 185 nucleotides
DVU0659: DVU0659 - hypothetical protein (TIGR), at 730,307 to 731,077
DVU0659
DVU0660: DVU0660 - phosphoesterase, putative (TIGR), at 731,288 to 731,854
DVU0660
DVU0661: DVU0661 - dihydrouridine synthase family protein (TIGR), at 732,072 to 733,070
DVU0661
DVU0662: cysE - serine O-acetyltransferase (TIGR), at 733,256 to 734,227
cysE
Position (kb) 732
733
734 Strain fitness (log2 ratio) -2
-1
0
1 at 731.140 kb on - strand at 731.158 kb on - strand at 731.177 kb on - strand at 731.200 kb on - strand at 731.235 kb on - strand at 731.248 kb on + strand at 731.286 kb on - strand at 731.419 kb on + strand, within DVU0660 at 731.439 kb on - strand, within DVU0660 at 731.645 kb on - strand, within DVU0660 at 731.673 kb on + strand, within DVU0660 at 731.696 kb on + strand, within DVU0660 at 731.770 kb on + strand, within DVU0660 at 731.785 kb on + strand, within DVU0660 at 731.793 kb on - strand, within DVU0660 at 731.864 kb on + strand at 731.872 kb on - strand at 732.072 kb on + strand at 732.075 kb on - strand at 732.080 kb on - strand at 732.080 kb on - strand at 732.093 kb on + strand at 732.103 kb on + strand at 732.109 kb on + strand at 732.112 kb on - strand at 732.112 kb on - strand at 732.185 kb on + strand, within DVU0661 at 732.186 kb on - strand, within DVU0661 at 732.217 kb on - strand, within DVU0661 at 732.234 kb on - strand, within DVU0661 at 732.290 kb on - strand, within DVU0661 at 732.303 kb on - strand, within DVU0661 at 732.303 kb on - strand, within DVU0661 at 732.602 kb on - strand, within DVU0661 at 732.764 kb on - strand, within DVU0661 at 732.979 kb on - strand at 733.008 kb on + strand at 733.072 kb on - strand at 733.123 kb on - strand at 733.151 kb on + strand at 733.159 kb on - strand at 733.173 kb on + strand at 733.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without vitamins with Casaminos 0.2% remove 731,140 - -1.4 731,158 - -0.0 731,177 - -0.1 731,200 - -2.5 731,235 - -0.2 731,248 + +0.4 731,286 - -0.5 731,419 + DVU0660 0.23 -0.6 731,439 - DVU0660 0.27 +0.3 731,645 - DVU0660 0.63 +0.4 731,673 + DVU0660 0.68 -0.8 731,696 + DVU0660 0.72 -1.2 731,770 + DVU0660 0.85 -0.4 731,785 + DVU0660 0.88 -1.5 731,793 - DVU0660 0.89 +0.6 731,864 + -0.2 731,872 - -0.8 732,072 + +0.3 732,075 - -0.2 732,080 - +0.8 732,080 - +0.2 732,093 + -0.8 732,103 + -0.3 732,109 + -0.3 732,112 - -1.3 732,112 - +0.1 732,185 + DVU0661 0.11 +0.1 732,186 - DVU0661 0.11 +0.5 732,217 - DVU0661 0.15 -0.2 732,234 - DVU0661 0.16 -0.1 732,290 - DVU0661 0.22 -0.1 732,303 - DVU0661 0.23 -0.3 732,303 - DVU0661 0.23 +0.3 732,602 - DVU0661 0.53 +0.1 732,764 - DVU0661 0.69 +0.3 732,979 - +0.0 733,008 + -1.6 733,072 - -0.0 733,123 - +0.3 733,151 + -2.6 733,159 - +0.6 733,173 + +0.2 733,312 + -0.5
Or see this region's nucleotide sequence