Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06350 and MMJJ_RS06355 are separated by 8 nucleotides MMJJ_RS06355 and MMJJ_RS06360 are separated by 11 nucleotides MMJJ_RS06360 and MMJJ_RS06365 are separated by 286 nucleotides
MMJJ_RS06350: MMJJ_RS06350 - methyl-coenzyme M reductase I operon protein C, at 1,176,421 to 1,177,017
_RS06350
MMJJ_RS06355: MMJJ_RS06355 - methyl-coenzyme M reductase operon protein D, at 1,177,026 to 1,177,505
_RS06355
MMJJ_RS06360: MMJJ_RS06360 - coenzyme-B sulfoethylthiotransferase subunit beta, at 1,177,517 to 1,178,848
_RS06360
MMJJ_RS06365: MMJJ_RS06365 - methyl coenzyme M reductase-arginine methyltransferase Mmp10, at 1,179,135 to 1,180,418
_RS06365
Position (kb)
1177
1178
1179 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6 at 1178.469 kb on - strand, within MMJJ_RS06360 at 1178.679 kb on + strand at 1178.737 kb on - strand at 1178.884 kb on + strand at 1178.889 kb on - strand at 1178.905 kb on + strand at 1178.905 kb on + strand at 1178.905 kb on + strand at 1178.941 kb on - strand at 1178.941 kb on - strand at 1178.942 kb on + strand at 1178.942 kb on + strand at 1178.942 kb on + strand at 1178.943 kb on - strand at 1178.943 kb on - strand at 1178.944 kb on + strand at 1178.972 kb on - strand at 1178.973 kb on + strand at 1178.977 kb on - strand at 1178.981 kb on - strand at 1178.993 kb on - strand at 1178.997 kb on + strand at 1179.000 kb on - strand at 1179.000 kb on - strand at 1179.000 kb on - strand at 1179.000 kb on - strand at 1179.000 kb on - strand at 1179.001 kb on + strand at 1179.001 kb on + strand at 1179.001 kb on + strand at 1179.001 kb on + strand at 1179.001 kb on + strand at 1179.001 kb on + strand at 1179.001 kb on + strand at 1179.003 kb on - strand at 1179.003 kb on - strand at 1179.004 kb on + strand at 1179.004 kb on + strand at 1179.011 kb on - strand at 1179.012 kb on + strand at 1179.012 kb on + strand at 1179.031 kb on - strand at 1179.032 kb on + strand at 1179.061 kb on + strand at 1179.065 kb on - strand at 1179.066 kb on + strand at 1179.066 kb on + strand at 1179.075 kb on - strand at 1179.079 kb on + strand at 1179.086 kb on - strand at 1179.097 kb on + strand at 1179.104 kb on - strand at 1179.107 kb on - strand at 1179.107 kb on - strand at 1179.135 kb on + strand at 1179.320 kb on + strand, within MMJJ_RS06365 at 1179.335 kb on - strand, within MMJJ_RS06365 at 1179.369 kb on + strand, within MMJJ_RS06365 at 1179.459 kb on + strand, within MMJJ_RS06365 at 1179.523 kb on - strand, within MMJJ_RS06365 at 1179.523 kb on - strand, within MMJJ_RS06365 at 1179.524 kb on + strand, within MMJJ_RS06365 at 1179.529 kb on + strand, within MMJJ_RS06365 at 1179.530 kb on - strand, within MMJJ_RS06365 at 1179.548 kb on + strand, within MMJJ_RS06365 at 1179.571 kb on + strand, within MMJJ_RS06365 at 1179.589 kb on - strand, within MMJJ_RS06365 at 1179.633 kb on + strand, within MMJJ_RS06365 at 1179.653 kb on - strand, within MMJJ_RS06365 at 1179.683 kb on + strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.754 kb on - strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365 at 1179.755 kb on + strand, within MMJJ_RS06365
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 1,178,469 - MMJJ_RS06360 0.71 -0.1 1,178,679 + +1.1 1,178,737 - +0.1 1,178,884 + +0.1 1,178,889 - +1.1 1,178,905 + +1.1 1,178,905 + -0.5 1,178,905 + -1.1 1,178,941 - -0.9 1,178,941 - -0.3 1,178,942 + -2.2 1,178,942 + +4.2 1,178,942 + +2.2 1,178,943 - -2.0 1,178,943 - -0.6 1,178,944 + +1.8 1,178,972 - -0.3 1,178,973 + +5.8 1,178,977 - +2.2 1,178,981 - +0.6 1,178,993 - -0.6 1,178,997 + -2.1 1,179,000 - +0.2 1,179,000 - +1.8 1,179,000 - +1.2 1,179,000 - +1.2 1,179,000 - -4.4 1,179,001 + -0.7 1,179,001 + -1.4 1,179,001 + +0.8 1,179,001 + -2.1 1,179,001 + -0.9 1,179,001 + -0.5 1,179,001 + +0.1 1,179,003 - +2.3 1,179,003 - -0.5 1,179,004 + +3.0 1,179,004 + -1.6 1,179,011 - -0.2 1,179,012 + +0.1 1,179,012 + +0.2 1,179,031 - -1.4 1,179,032 + -0.6 1,179,061 + -0.3 1,179,065 - +4.3 1,179,066 + +0.1 1,179,066 + -0.7 1,179,075 - +0.4 1,179,079 + +1.1 1,179,086 - +1.2 1,179,097 + -2.6 1,179,104 - -0.2 1,179,107 - -1.5 1,179,107 - -2.2 1,179,135 + +0.7 1,179,320 + MMJJ_RS06365 0.14 -0.7 1,179,335 - MMJJ_RS06365 0.16 -1.2 1,179,369 + MMJJ_RS06365 0.18 -0.2 1,179,459 + MMJJ_RS06365 0.25 -3.0 1,179,523 - MMJJ_RS06365 0.30 +3.6 1,179,523 - MMJJ_RS06365 0.30 +1.7 1,179,524 + MMJJ_RS06365 0.30 -0.6 1,179,529 + MMJJ_RS06365 0.31 +2.1 1,179,530 - MMJJ_RS06365 0.31 -3.6 1,179,548 + MMJJ_RS06365 0.32 +1.1 1,179,571 + MMJJ_RS06365 0.34 -0.7 1,179,589 - MMJJ_RS06365 0.35 -0.7 1,179,633 + MMJJ_RS06365 0.39 -3.2 1,179,653 - MMJJ_RS06365 0.40 -0.2 1,179,683 + MMJJ_RS06365 0.43 -0.9 1,179,754 - MMJJ_RS06365 0.48 +0.2 1,179,754 - MMJJ_RS06365 0.48 -0.3 1,179,754 - MMJJ_RS06365 0.48 -0.4 1,179,754 - MMJJ_RS06365 0.48 +0.6 1,179,754 - MMJJ_RS06365 0.48 -0.7 1,179,754 - MMJJ_RS06365 0.48 +1.7 1,179,754 - MMJJ_RS06365 0.48 +2.3 1,179,754 - MMJJ_RS06365 0.48 -0.3 1,179,754 - MMJJ_RS06365 0.48 +0.0 1,179,754 - MMJJ_RS06365 0.48 -3.7 1,179,755 + MMJJ_RS06365 0.48 +0.4 1,179,755 + MMJJ_RS06365 0.48 -0.8 1,179,755 + MMJJ_RS06365 0.48 -0.7 1,179,755 + MMJJ_RS06365 0.48 +0.5 1,179,755 + MMJJ_RS06365 0.48 -0.6 1,179,755 + MMJJ_RS06365 0.48 +0.7 1,179,755 + MMJJ_RS06365 0.48 -1.7 1,179,755 + MMJJ_RS06365 0.48 -4.1 1,179,755 + MMJJ_RS06365 0.48 +0.1 1,179,755 + MMJJ_RS06365 0.48 -0.3
Or see this region's nucleotide sequence