Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05130 and MMJJ_RS05135 are separated by 18 nucleotides MMJJ_RS05135 and MMJJ_RS05140 are separated by 108 nucleotides MMJJ_RS05140 and MMJJ_RS05145 are separated by 62 nucleotides MMJJ_RS05145 and MMJJ_RS05150 are separated by 229 nucleotides
MMJJ_RS05130: MMJJ_RS05130 - translation initiation factor IF-6, at 950,566 to 951,249
_RS05130
MMJJ_RS05135: MMJJ_RS05135 - 50S ribosomal protein L18Ae, at 951,268 to 951,498
_RS05135
MMJJ_RS05140: MMJJ_RS05140 - DUF2666 domain-containing protein, at 951,607 to 952,029
_RS05140
MMJJ_RS05145: MMJJ_RS05145 - 5,10-methylenetetrahydromethanopterin reductase, at 952,092 to 953,054
_RS05145
MMJJ_RS05150: MMJJ_RS05150 - 5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH, at 953,284 to 954,360
_RS05150
Position (kb) 952
953
954 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 951.243 kb on + strand at 951.248 kb on + strand at 951.264 kb on - strand at 951.265 kb on + strand at 951.449 kb on - strand, within MMJJ_RS05135 at 951.496 kb on - strand at 951.496 kb on - strand at 951.505 kb on - strand at 951.514 kb on - strand at 951.515 kb on + strand at 951.540 kb on - strand at 951.545 kb on - strand at 951.546 kb on + strand at 951.547 kb on - strand at 951.547 kb on - strand at 951.550 kb on + strand at 951.554 kb on - strand at 951.563 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.565 kb on - strand at 951.577 kb on - strand at 951.579 kb on - strand at 951.579 kb on - strand at 951.580 kb on + strand at 951.583 kb on + strand at 951.606 kb on - strand at 951.623 kb on - strand at 951.653 kb on + strand, within MMJJ_RS05140 at 951.684 kb on - strand, within MMJJ_RS05140 at 951.801 kb on + strand, within MMJJ_RS05140 at 951.825 kb on - strand, within MMJJ_RS05140 at 951.825 kb on - strand, within MMJJ_RS05140 at 951.838 kb on + strand, within MMJJ_RS05140 at 951.877 kb on + strand, within MMJJ_RS05140 at 951.903 kb on - strand, within MMJJ_RS05140 at 951.954 kb on + strand, within MMJJ_RS05140 at 951.987 kb on - strand at 951.988 kb on + strand at 952.004 kb on + strand at 952.090 kb on + strand at 952.390 kb on + strand at 952.747 kb on + strand at 953.096 kb on - strand at 953.104 kb on + strand at 953.152 kb on - strand at 953.152 kb on - strand at 953.237 kb on + strand at 953.237 kb on + strand at 953.241 kb on + strand at 953.340 kb on + strand at 953.350 kb on + strand at 953.391 kb on - strand at 953.392 kb on + strand, within MMJJ_RS05150 at 953.460 kb on - strand, within MMJJ_RS05150 at 953.473 kb on + strand, within MMJJ_RS05150 at 953.553 kb on - strand, within MMJJ_RS05150 at 953.575 kb on + strand, within MMJJ_RS05150 at 953.584 kb on + strand, within MMJJ_RS05150 at 953.643 kb on - strand, within MMJJ_RS05150 at 953.643 kb on - strand, within MMJJ_RS05150 at 953.644 kb on + strand, within MMJJ_RS05150 at 953.679 kb on + strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.680 kb on - strand, within MMJJ_RS05150 at 953.681 kb on + strand, within MMJJ_RS05150 at 953.681 kb on + strand, within MMJJ_RS05150 at 953.692 kb on - strand, within MMJJ_RS05150 at 953.736 kb on - strand, within MMJJ_RS05150 at 953.736 kb on - strand, within MMJJ_RS05150 at 953.737 kb on + strand, within MMJJ_RS05150 at 953.822 kb on - strand, within MMJJ_RS05150 at 953.823 kb on + strand, within MMJJ_RS05150 at 953.823 kb on + strand, within MMJJ_RS05150 at 953.871 kb on - strand, within MMJJ_RS05150 at 953.872 kb on + strand, within MMJJ_RS05150 at 953.872 kb on + strand, within MMJJ_RS05150 at 953.901 kb on - strand, within MMJJ_RS05150 at 953.902 kb on + strand, within MMJJ_RS05150 at 953.950 kb on + strand, within MMJJ_RS05150 at 954.029 kb on - strand, within MMJJ_RS05150 at 954.030 kb on + strand, within MMJJ_RS05150
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 951,243 + +0.7 951,248 + +0.7 951,264 - +1.7 951,265 + +0.3 951,449 - MMJJ_RS05135 0.78 -2.0 951,496 - -1.1 951,496 - +1.8 951,505 - -0.7 951,514 - -0.5 951,515 + -4.5 951,540 - -1.1 951,545 - -1.5 951,546 + -1.2 951,547 - -0.8 951,547 - +1.2 951,550 + +1.4 951,554 - +2.2 951,563 - -1.4 951,565 - -0.3 951,565 - +1.1 951,565 - +2.8 951,565 - +1.7 951,577 - -0.3 951,579 - -0.1 951,579 - -2.8 951,580 + +0.1 951,583 + -2.3 951,606 - +1.2 951,623 - +1.3 951,653 + MMJJ_RS05140 0.11 +0.9 951,684 - MMJJ_RS05140 0.18 -1.9 951,801 + MMJJ_RS05140 0.46 +1.1 951,825 - MMJJ_RS05140 0.52 +3.1 951,825 - MMJJ_RS05140 0.52 -2.2 951,838 + MMJJ_RS05140 0.55 -0.5 951,877 + MMJJ_RS05140 0.64 +1.7 951,903 - MMJJ_RS05140 0.70 -1.5 951,954 + MMJJ_RS05140 0.82 -2.2 951,987 - +1.6 951,988 + +0.3 952,004 + +0.4 952,090 + -1.1 952,390 + -0.6 952,747 + +1.3 953,096 - -0.3 953,104 + -0.3 953,152 - -3.4 953,152 - +1.4 953,237 + -0.1 953,237 + -0.9 953,241 + +1.2 953,340 + +2.5 953,350 + -1.7 953,391 - +1.5 953,392 + MMJJ_RS05150 0.10 -1.9 953,460 - MMJJ_RS05150 0.16 +1.2 953,473 + MMJJ_RS05150 0.18 -1.7 953,553 - MMJJ_RS05150 0.25 +2.1 953,575 + MMJJ_RS05150 0.27 +3.0 953,584 + MMJJ_RS05150 0.28 +0.8 953,643 - MMJJ_RS05150 0.33 -2.5 953,643 - MMJJ_RS05150 0.33 -2.5 953,644 + MMJJ_RS05150 0.33 -0.8 953,679 + MMJJ_RS05150 0.37 -1.7 953,680 - MMJJ_RS05150 0.37 +1.1 953,680 - MMJJ_RS05150 0.37 +0.4 953,680 - MMJJ_RS05150 0.37 +0.2 953,680 - MMJJ_RS05150 0.37 +0.7 953,680 - MMJJ_RS05150 0.37 -3.5 953,681 + MMJJ_RS05150 0.37 +2.2 953,681 + MMJJ_RS05150 0.37 -0.5 953,692 - MMJJ_RS05150 0.38 +0.8 953,736 - MMJJ_RS05150 0.42 +1.6 953,736 - MMJJ_RS05150 0.42 +1.5 953,737 + MMJJ_RS05150 0.42 +3.0 953,822 - MMJJ_RS05150 0.50 +2.3 953,823 + MMJJ_RS05150 0.50 +1.4 953,823 + MMJJ_RS05150 0.50 -2.5 953,871 - MMJJ_RS05150 0.55 +0.1 953,872 + MMJJ_RS05150 0.55 +0.8 953,872 + MMJJ_RS05150 0.55 -0.8 953,901 - MMJJ_RS05150 0.57 +0.9 953,902 + MMJJ_RS05150 0.57 -2.4 953,950 + MMJJ_RS05150 0.62 +1.3 954,029 - MMJJ_RS05150 0.69 +2.2 954,030 + MMJJ_RS05150 0.69 -0.9
Or see this region's nucleotide sequence