Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03135 and MMJJ_RS03140 are separated by 14 nucleotides MMJJ_RS03140 and MMJJ_RS03145 are separated by 29 nucleotides MMJJ_RS03145 and MMJJ_RS03150 are separated by 105 nucleotides
MMJJ_RS03135: MMJJ_RS03135 - CBS domain-containing protein, at 592,485 to 592,898
_RS03135
MMJJ_RS03140: MMJJ_RS03140 - tRNA-dihydrouridine synthase, at 592,913 to 593,683
_RS03140
MMJJ_RS03145: MMJJ_RS03145 - GTP cyclohydrolase IIa, at 593,713 to 594,513
_RS03145
MMJJ_RS03150: MMJJ_RS03150 - 2-phospho-L-lactate transferase, at 594,619 to 595,548
_RS03150
Position (kb) 593
594
595 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 592.868 kb on - strand at 592.891 kb on - strand at 592.892 kb on + strand at 592.892 kb on + strand at 592.910 kb on - strand at 592.911 kb on + strand at 592.921 kb on - strand at 592.921 kb on - strand at 592.921 kb on - strand at 592.922 kb on + strand at 592.935 kb on + strand at 592.940 kb on + strand at 592.949 kb on - strand at 593.079 kb on + strand, within MMJJ_RS03140 at 593.105 kb on + strand, within MMJJ_RS03140 at 593.120 kb on - strand, within MMJJ_RS03140 at 593.120 kb on - strand, within MMJJ_RS03140 at 593.134 kb on + strand, within MMJJ_RS03140 at 593.135 kb on - strand, within MMJJ_RS03140 at 593.135 kb on - strand, within MMJJ_RS03140 at 593.136 kb on + strand, within MMJJ_RS03140 at 593.136 kb on + strand, within MMJJ_RS03140 at 593.136 kb on + strand, within MMJJ_RS03140 at 593.140 kb on - strand, within MMJJ_RS03140 at 593.253 kb on + strand, within MMJJ_RS03140 at 593.253 kb on + strand, within MMJJ_RS03140 at 593.307 kb on + strand, within MMJJ_RS03140 at 593.371 kb on - strand, within MMJJ_RS03140 at 593.411 kb on - strand, within MMJJ_RS03140 at 593.412 kb on + strand, within MMJJ_RS03140 at 593.480 kb on - strand, within MMJJ_RS03140 at 593.480 kb on - strand, within MMJJ_RS03140 at 593.492 kb on + strand, within MMJJ_RS03140 at 593.492 kb on + strand, within MMJJ_RS03140 at 593.525 kb on + strand, within MMJJ_RS03140 at 593.584 kb on - strand, within MMJJ_RS03140 at 593.585 kb on + strand, within MMJJ_RS03140 at 593.592 kb on - strand, within MMJJ_RS03140 at 593.593 kb on + strand, within MMJJ_RS03140 at 593.672 kb on - strand at 593.697 kb on - strand at 593.698 kb on + strand at 593.715 kb on + strand at 593.729 kb on - strand at 593.729 kb on - strand at 593.729 kb on - strand at 593.729 kb on - strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.730 kb on + strand at 593.735 kb on - strand at 593.736 kb on + strand at 593.778 kb on + strand at 593.946 kb on - strand at 593.959 kb on + strand, within MMJJ_RS03145 at 594.755 kb on + strand, within MMJJ_RS03150 at 594.763 kb on - strand, within MMJJ_RS03150 at 594.764 kb on + strand, within MMJJ_RS03150 at 594.776 kb on - strand, within MMJJ_RS03150 at 595.044 kb on - strand, within MMJJ_RS03150 at 595.389 kb on + strand, within MMJJ_RS03150 at 595.422 kb on - strand, within MMJJ_RS03150 at 595.432 kb on + strand, within MMJJ_RS03150 at 595.432 kb on + strand, within MMJJ_RS03150 at 595.433 kb on - strand, within MMJJ_RS03150 at 595.433 kb on - strand, within MMJJ_RS03150 at 595.433 kb on - strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.434 kb on + strand, within MMJJ_RS03150 at 595.437 kb on - strand, within MMJJ_RS03150 at 595.454 kb on + strand, within MMJJ_RS03150
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 592,868 - +0.3 592,891 - +3.5 592,892 + -1.2 592,892 + -0.1 592,910 - -1.1 592,911 + -0.8 592,921 - -1.2 592,921 - +0.9 592,921 - -0.6 592,922 + -2.0 592,935 + +1.3 592,940 + -1.6 592,949 - -3.4 593,079 + MMJJ_RS03140 0.22 -2.6 593,105 + MMJJ_RS03140 0.25 -2.9 593,120 - MMJJ_RS03140 0.27 +1.6 593,120 - MMJJ_RS03140 0.27 -1.0 593,134 + MMJJ_RS03140 0.29 -2.3 593,135 - MMJJ_RS03140 0.29 -0.5 593,135 - MMJJ_RS03140 0.29 -4.1 593,136 + MMJJ_RS03140 0.29 -1.6 593,136 + MMJJ_RS03140 0.29 -2.2 593,136 + MMJJ_RS03140 0.29 -2.3 593,140 - MMJJ_RS03140 0.29 +0.8 593,253 + MMJJ_RS03140 0.44 -1.8 593,253 + MMJJ_RS03140 0.44 -1.3 593,307 + MMJJ_RS03140 0.51 -2.5 593,371 - MMJJ_RS03140 0.59 -2.2 593,411 - MMJJ_RS03140 0.65 -1.2 593,412 + MMJJ_RS03140 0.65 -3.4 593,480 - MMJJ_RS03140 0.74 -1.6 593,480 - MMJJ_RS03140 0.74 -3.9 593,492 + MMJJ_RS03140 0.75 -2.6 593,492 + MMJJ_RS03140 0.75 -1.5 593,525 + MMJJ_RS03140 0.79 -1.6 593,584 - MMJJ_RS03140 0.87 -0.0 593,585 + MMJJ_RS03140 0.87 -1.9 593,592 - MMJJ_RS03140 0.88 -2.8 593,593 + MMJJ_RS03140 0.88 -0.4 593,672 - -2.7 593,697 - -0.5 593,698 + -2.5 593,715 + -2.1 593,729 - +2.5 593,729 - +3.0 593,729 - +2.9 593,729 - +1.5 593,730 + +1.1 593,730 + +0.1 593,730 + -3.0 593,730 + +2.7 593,730 + -0.3 593,730 + -0.1 593,730 + +2.0 593,735 - -3.2 593,736 + -0.8 593,778 + -2.0 593,946 - -0.6 593,959 + MMJJ_RS03145 0.31 -0.3 594,755 + MMJJ_RS03150 0.15 -0.2 594,763 - MMJJ_RS03150 0.15 -0.8 594,764 + MMJJ_RS03150 0.16 +0.8 594,776 - MMJJ_RS03150 0.17 -1.8 595,044 - MMJJ_RS03150 0.46 -1.5 595,389 + MMJJ_RS03150 0.83 -2.8 595,422 - MMJJ_RS03150 0.86 +0.2 595,432 + MMJJ_RS03150 0.87 -0.8 595,432 + MMJJ_RS03150 0.87 -2.1 595,433 - MMJJ_RS03150 0.88 -1.3 595,433 - MMJJ_RS03150 0.88 -0.8 595,433 - MMJJ_RS03150 0.88 -2.7 595,434 + MMJJ_RS03150 0.88 -1.6 595,434 + MMJJ_RS03150 0.88 -0.2 595,434 + MMJJ_RS03150 0.88 -1.5 595,434 + MMJJ_RS03150 0.88 -2.5 595,434 + MMJJ_RS03150 0.88 -0.6 595,437 - MMJJ_RS03150 0.88 +1.0 595,454 + MMJJ_RS03150 0.90 -1.0
Or see this region's nucleotide sequence