Experiment: Formate N-free with 2mM Adenosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01140 and MMJJ_RS01150 are separated by 331 nucleotides MMJJ_RS01150 and MMJJ_RS01155 are separated by 35 nucleotides MMJJ_RS01155 and MMJJ_RS01160 are separated by 271 nucleotides
MMJJ_RS01140: MMJJ_RS01140 - helix-turn-helix domain-containing protein, at 222,633 to 222,836
_RS01140
MMJJ_RS01150: MMJJ_RS01150 - hypothetical protein, at 223,168 to 223,587
_RS01150
MMJJ_RS01155: MMJJ_RS01155 - hypothetical protein, at 223,623 to 224,075
_RS01155
MMJJ_RS01160: MMJJ_RS01160 - AAA family ATPase, at 224,347 to 225,390
_RS01160
Position (kb) 223
224
225 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 222.628 kb on - strand at 222.629 kb on + strand at 222.661 kb on - strand, within MMJJ_RS01140 at 222.662 kb on + strand, within MMJJ_RS01140 at 222.675 kb on - strand, within MMJJ_RS01140 at 222.675 kb on - strand, within MMJJ_RS01140 at 222.705 kb on - strand, within MMJJ_RS01140 at 222.708 kb on + strand, within MMJJ_RS01140 at 222.725 kb on - strand, within MMJJ_RS01140 at 222.737 kb on + strand, within MMJJ_RS01140 at 222.815 kb on + strand, within MMJJ_RS01140 at 222.815 kb on + strand, within MMJJ_RS01140 at 222.894 kb on - strand at 222.910 kb on - strand at 222.910 kb on - strand at 223.030 kb on - strand at 223.031 kb on + strand at 223.098 kb on + strand at 223.137 kb on - strand at 223.144 kb on - strand at 223.145 kb on + strand at 223.179 kb on - strand at 223.179 kb on - strand at 223.184 kb on + strand at 223.184 kb on + strand at 223.258 kb on - strand, within MMJJ_RS01150 at 223.259 kb on + strand, within MMJJ_RS01150 at 223.259 kb on + strand, within MMJJ_RS01150 at 223.270 kb on - strand, within MMJJ_RS01150 at 223.346 kb on - strand, within MMJJ_RS01150 at 223.492 kb on + strand, within MMJJ_RS01150 at 223.620 kb on - strand at 223.620 kb on - strand at 223.647 kb on + strand at 223.649 kb on - strand at 223.649 kb on - strand at 223.649 kb on - strand at 223.650 kb on + strand at 223.651 kb on - strand at 223.651 kb on - strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.652 kb on + strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.750 kb on - strand, within MMJJ_RS01155 at 223.782 kb on - strand, within MMJJ_RS01155 at 223.929 kb on + strand, within MMJJ_RS01155 at 223.944 kb on + strand, within MMJJ_RS01155 at 223.950 kb on - strand, within MMJJ_RS01155 at 223.952 kb on - strand, within MMJJ_RS01155 at 224.035 kb on - strand at 224.060 kb on + strand at 224.081 kb on - strand at 224.222 kb on - strand at 224.223 kb on + strand at 224.265 kb on + strand at 224.267 kb on - strand at 224.268 kb on + strand at 224.269 kb on - strand at 224.271 kb on - strand at 224.272 kb on + strand at 224.272 kb on + strand at 224.272 kb on + strand at 224.278 kb on - strand at 224.279 kb on + strand at 224.279 kb on + strand at 224.306 kb on + strand at 224.355 kb on + strand at 224.361 kb on + strand at 224.380 kb on - strand at 224.591 kb on - strand, within MMJJ_RS01160 at 224.688 kb on - strand, within MMJJ_RS01160 at 224.689 kb on + strand, within MMJJ_RS01160 at 224.717 kb on - strand, within MMJJ_RS01160 at 224.753 kb on - strand, within MMJJ_RS01160 at 224.753 kb on - strand, within MMJJ_RS01160 at 224.753 kb on - strand, within MMJJ_RS01160 at 224.753 kb on - strand, within MMJJ_RS01160 at 224.753 kb on - strand, within MMJJ_RS01160 at 224.753 kb on - strand, within MMJJ_RS01160 at 224.754 kb on + strand, within MMJJ_RS01160 at 224.754 kb on + strand, within MMJJ_RS01160 at 224.754 kb on + strand, within MMJJ_RS01160 at 224.754 kb on + strand, within MMJJ_RS01160 at 224.754 kb on + strand, within MMJJ_RS01160 at 224.755 kb on - strand, within MMJJ_RS01160 at 224.755 kb on - strand, within MMJJ_RS01160 at 224.755 kb on - strand, within MMJJ_RS01160 at 224.756 kb on + strand, within MMJJ_RS01160 at 224.756 kb on + strand, within MMJJ_RS01160 at 224.756 kb on + strand, within MMJJ_RS01160 at 224.756 kb on + strand, within MMJJ_RS01160 at 224.756 kb on + strand, within MMJJ_RS01160 at 224.761 kb on + strand, within MMJJ_RS01160 at 224.761 kb on + strand, within MMJJ_RS01160 at 224.800 kb on + strand, within MMJJ_RS01160 at 224.845 kb on - strand, within MMJJ_RS01160 at 224.859 kb on - strand, within MMJJ_RS01160 at 224.859 kb on - strand, within MMJJ_RS01160 at 224.869 kb on - strand, within MMJJ_RS01160 at 224.869 kb on - strand, within MMJJ_RS01160 at 224.870 kb on + strand, within MMJJ_RS01160 at 224.870 kb on + strand, within MMJJ_RS01160 at 224.942 kb on - strand, within MMJJ_RS01160 at 224.942 kb on - strand, within MMJJ_RS01160 at 224.976 kb on - strand, within MMJJ_RS01160 at 224.976 kb on - strand, within MMJJ_RS01160 at 224.977 kb on + strand, within MMJJ_RS01160 at 225.020 kb on + strand, within MMJJ_RS01160 at 225.028 kb on - strand, within MMJJ_RS01160 at 225.030 kb on - strand, within MMJJ_RS01160 at 225.030 kb on - strand, within MMJJ_RS01160 at 225.030 kb on - strand, within MMJJ_RS01160 at 225.030 kb on - strand, within MMJJ_RS01160 at 225.031 kb on + strand, within MMJJ_RS01160 at 225.031 kb on + strand, within MMJJ_RS01160 at 225.031 kb on + strand, within MMJJ_RS01160 at 225.031 kb on + strand, within MMJJ_RS01160 at 225.031 kb on + strand, within MMJJ_RS01160 at 225.031 kb on + strand, within MMJJ_RS01160 at 225.032 kb on - strand, within MMJJ_RS01160 at 225.032 kb on - strand, within MMJJ_RS01160 at 225.032 kb on - strand, within MMJJ_RS01160 at 225.032 kb on - strand, within MMJJ_RS01160 at 225.032 kb on - strand, within MMJJ_RS01160 at 225.032 kb on - strand, within MMJJ_RS01160 at 225.032 kb on - strand, within MMJJ_RS01160 at 225.033 kb on + strand, within MMJJ_RS01160 at 225.033 kb on + strand, within MMJJ_RS01160 at 225.033 kb on + strand, within MMJJ_RS01160 at 225.033 kb on + strand, within MMJJ_RS01160 at 225.033 kb on + strand, within MMJJ_RS01160 at 225.033 kb on + strand, within MMJJ_RS01160 at 225.033 kb on + strand, within MMJJ_RS01160 at 225.034 kb on - strand, within MMJJ_RS01160 at 225.034 kb on - strand, within MMJJ_RS01160 at 225.034 kb on - strand, within MMJJ_RS01160 at 225.034 kb on - strand, within MMJJ_RS01160 at 225.034 kb on - strand, within MMJJ_RS01160 at 225.035 kb on + strand, within MMJJ_RS01160 at 225.035 kb on + strand, within MMJJ_RS01160 at 225.039 kb on - strand, within MMJJ_RS01160 at 225.039 kb on - strand, within MMJJ_RS01160 at 225.051 kb on - strand, within MMJJ_RS01160 at 225.055 kb on + strand, within MMJJ_RS01160
Per-strain Table
Position Strand Gene LocusTag Fraction Formate N-free with 2mM Adenosine remove 222,628 - +0.2 222,629 + -3.7 222,661 - MMJJ_RS01140 0.14 -1.3 222,662 + MMJJ_RS01140 0.14 +1.8 222,675 - MMJJ_RS01140 0.21 +2.8 222,675 - MMJJ_RS01140 0.21 -1.5 222,705 - MMJJ_RS01140 0.35 -2.4 222,708 + MMJJ_RS01140 0.37 -0.6 222,725 - MMJJ_RS01140 0.45 +0.6 222,737 + MMJJ_RS01140 0.51 +1.6 222,815 + MMJJ_RS01140 0.89 +0.6 222,815 + MMJJ_RS01140 0.89 +0.7 222,894 - +0.8 222,910 - -0.8 222,910 - +1.1 223,030 - +1.5 223,031 + -2.3 223,098 + -1.2 223,137 - -1.5 223,144 - +4.7 223,145 + -1.5 223,179 - -0.2 223,179 - +0.7 223,184 + +3.2 223,184 + -1.3 223,258 - MMJJ_RS01150 0.21 +1.1 223,259 + MMJJ_RS01150 0.22 -0.9 223,259 + MMJJ_RS01150 0.22 -1.5 223,270 - MMJJ_RS01150 0.24 -1.8 223,346 - MMJJ_RS01150 0.42 +1.8 223,492 + MMJJ_RS01150 0.77 +0.5 223,620 - -1.1 223,620 - +1.1 223,647 + -0.7 223,649 - +1.0 223,649 - -0.4 223,649 - +2.5 223,650 + +0.9 223,651 - +0.7 223,651 - -0.5 223,652 + -1.4 223,652 + -1.0 223,652 + +1.0 223,652 + -1.4 223,652 + -2.7 223,652 + +2.3 223,652 + +1.1 223,652 + +2.6 223,652 + -0.7 223,652 + +3.2 223,652 + +0.5 223,656 + +2.5 223,656 + +0.3 223,750 - MMJJ_RS01155 0.28 +1.1 223,782 - MMJJ_RS01155 0.35 +0.8 223,929 + MMJJ_RS01155 0.68 +2.2 223,944 + MMJJ_RS01155 0.71 +3.4 223,950 - MMJJ_RS01155 0.72 +1.7 223,952 - MMJJ_RS01155 0.73 -0.6 224,035 - +1.8 224,060 + -1.6 224,081 - -2.4 224,222 - +1.3 224,223 + -1.1 224,265 + +0.7 224,267 - +0.5 224,268 + -0.8 224,269 - +0.7 224,271 - -1.9 224,272 + +2.6 224,272 + +0.9 224,272 + -0.4 224,278 - +0.8 224,279 + +1.3 224,279 + -0.3 224,306 + -0.0 224,355 + -1.1 224,361 + +1.6 224,380 - -1.4 224,591 - MMJJ_RS01160 0.23 -2.5 224,688 - MMJJ_RS01160 0.33 +2.7 224,689 + MMJJ_RS01160 0.33 +2.8 224,717 - MMJJ_RS01160 0.35 -0.1 224,753 - MMJJ_RS01160 0.39 +0.7 224,753 - MMJJ_RS01160 0.39 +0.2 224,753 - MMJJ_RS01160 0.39 +1.6 224,753 - MMJJ_RS01160 0.39 +1.9 224,753 - MMJJ_RS01160 0.39 -1.7 224,753 - MMJJ_RS01160 0.39 -0.3 224,754 + MMJJ_RS01160 0.39 +2.8 224,754 + MMJJ_RS01160 0.39 -1.8 224,754 + MMJJ_RS01160 0.39 -0.8 224,754 + MMJJ_RS01160 0.39 -1.3 224,754 + MMJJ_RS01160 0.39 -0.5 224,755 - MMJJ_RS01160 0.39 -1.1 224,755 - MMJJ_RS01160 0.39 -2.1 224,755 - MMJJ_RS01160 0.39 +3.0 224,756 + MMJJ_RS01160 0.39 +0.5 224,756 + MMJJ_RS01160 0.39 +1.5 224,756 + MMJJ_RS01160 0.39 +0.4 224,756 + MMJJ_RS01160 0.39 -1.0 224,756 + MMJJ_RS01160 0.39 -0.7 224,761 + MMJJ_RS01160 0.40 -1.4 224,761 + MMJJ_RS01160 0.40 -1.7 224,800 + MMJJ_RS01160 0.43 -0.8 224,845 - MMJJ_RS01160 0.48 +1.8 224,859 - MMJJ_RS01160 0.49 +0.5 224,859 - MMJJ_RS01160 0.49 -2.4 224,869 - MMJJ_RS01160 0.50 -2.4 224,869 - MMJJ_RS01160 0.50 +0.6 224,870 + MMJJ_RS01160 0.50 -0.1 224,870 + MMJJ_RS01160 0.50 -2.5 224,942 - MMJJ_RS01160 0.57 +1.4 224,942 - MMJJ_RS01160 0.57 -0.3 224,976 - MMJJ_RS01160 0.60 +2.5 224,976 - MMJJ_RS01160 0.60 +1.0 224,977 + MMJJ_RS01160 0.60 +2.9 225,020 + MMJJ_RS01160 0.64 +1.1 225,028 - MMJJ_RS01160 0.65 -0.2 225,030 - MMJJ_RS01160 0.65 -1.7 225,030 - MMJJ_RS01160 0.65 +0.3 225,030 - MMJJ_RS01160 0.65 -1.3 225,030 - MMJJ_RS01160 0.65 -0.1 225,031 + MMJJ_RS01160 0.66 +0.6 225,031 + MMJJ_RS01160 0.66 +0.2 225,031 + MMJJ_RS01160 0.66 +2.0 225,031 + MMJJ_RS01160 0.66 -1.3 225,031 + MMJJ_RS01160 0.66 -0.6 225,031 + MMJJ_RS01160 0.66 +0.9 225,032 - MMJJ_RS01160 0.66 +1.8 225,032 - MMJJ_RS01160 0.66 +1.9 225,032 - MMJJ_RS01160 0.66 +0.2 225,032 - MMJJ_RS01160 0.66 +2.5 225,032 - MMJJ_RS01160 0.66 +1.7 225,032 - MMJJ_RS01160 0.66 -0.5 225,032 - MMJJ_RS01160 0.66 +2.7 225,033 + MMJJ_RS01160 0.66 -0.9 225,033 + MMJJ_RS01160 0.66 +3.3 225,033 + MMJJ_RS01160 0.66 +0.7 225,033 + MMJJ_RS01160 0.66 -3.1 225,033 + MMJJ_RS01160 0.66 -1.6 225,033 + MMJJ_RS01160 0.66 +2.9 225,033 + MMJJ_RS01160 0.66 -2.4 225,034 - MMJJ_RS01160 0.66 +1.5 225,034 - MMJJ_RS01160 0.66 +1.8 225,034 - MMJJ_RS01160 0.66 +1.1 225,034 - MMJJ_RS01160 0.66 +0.7 225,034 - MMJJ_RS01160 0.66 +1.7 225,035 + MMJJ_RS01160 0.66 -0.4 225,035 + MMJJ_RS01160 0.66 -2.4 225,039 - MMJJ_RS01160 0.66 +0.7 225,039 - MMJJ_RS01160 0.66 -2.2 225,051 - MMJJ_RS01160 0.67 -1.0 225,055 + MMJJ_RS01160 0.68 -1.1
Or see this region's nucleotide sequence