Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS15110 and LRK54_RS15115 are separated by 55 nucleotides LRK54_RS15115 and LRK54_RS15120 are separated by 17 nucleotides LRK54_RS15120 and LRK54_RS15125 are separated by 100 nucleotides LRK54_RS15125 and LRK54_RS15130 are separated by 4 nucleotides
LRK54_RS15110: LRK54_RS15110 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,337,469 to 3,337,948
_RS15110
LRK54_RS15115: LRK54_RS15115 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,338,004 to 3,338,834
_RS15115
LRK54_RS15120: LRK54_RS15120 - pantoate--beta-alanine ligase, at 3,338,852 to 3,339,697
_RS15120
LRK54_RS15125: LRK54_RS15125 - aspartate 1-decarboxylase, at 3,339,798 to 3,340,190
_RS15125
LRK54_RS15130: LRK54_RS15130 - tRNA epoxyqueuosine(34) reductase QueG, at 3,340,195 to 3,341,292
_RS15130
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3337.971 kb on + strand at 3338.007 kb on + strand at 3339.708 kb on + strand at 3339.756 kb on + strand at 3340.344 kb on - strand, within LRK54_RS15130 at 3340.344 kb on - strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.586 kb on + strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.587 kb on - strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.645 kb on + strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130 at 3340.646 kb on - strand, within LRK54_RS15130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 3,337,971 + -0.2 3,338,007 + -0.1 3,339,708 + -0.3 3,339,756 + -0.1 3,340,344 - LRK54_RS15130 0.14 +0.7 3,340,344 - LRK54_RS15130 0.14 +0.5 3,340,586 + LRK54_RS15130 0.36 +1.7 3,340,586 + LRK54_RS15130 0.36 +0.2 3,340,586 + LRK54_RS15130 0.36 -0.3 3,340,586 + LRK54_RS15130 0.36 -0.0 3,340,586 + LRK54_RS15130 0.36 +0.7 3,340,586 + LRK54_RS15130 0.36 +1.9 3,340,586 + LRK54_RS15130 0.36 -0.3 3,340,586 + LRK54_RS15130 0.36 +3.6 3,340,586 + LRK54_RS15130 0.36 +1.0 3,340,586 + LRK54_RS15130 0.36 -0.8 3,340,586 + LRK54_RS15130 0.36 -0.5 3,340,586 + LRK54_RS15130 0.36 +0.3 3,340,586 + LRK54_RS15130 0.36 -0.8 3,340,587 - LRK54_RS15130 0.36 +1.7 3,340,587 - LRK54_RS15130 0.36 -0.6 3,340,587 - LRK54_RS15130 0.36 -1.5 3,340,587 - LRK54_RS15130 0.36 -1.1 3,340,587 - LRK54_RS15130 0.36 -0.1 3,340,587 - LRK54_RS15130 0.36 -0.3 3,340,587 - LRK54_RS15130 0.36 -0.6 3,340,587 - LRK54_RS15130 0.36 -1.0 3,340,587 - LRK54_RS15130 0.36 +1.5 3,340,587 - LRK54_RS15130 0.36 +0.5 3,340,587 - LRK54_RS15130 0.36 -0.4 3,340,587 - LRK54_RS15130 0.36 -1.5 3,340,587 - LRK54_RS15130 0.36 -0.4 3,340,645 + LRK54_RS15130 0.41 -0.1 3,340,645 + LRK54_RS15130 0.41 -1.2 3,340,645 + LRK54_RS15130 0.41 +0.3 3,340,645 + LRK54_RS15130 0.41 -0.7 3,340,645 + LRK54_RS15130 0.41 -0.1 3,340,645 + LRK54_RS15130 0.41 -0.1 3,340,645 + LRK54_RS15130 0.41 +0.3 3,340,645 + LRK54_RS15130 0.41 +0.7 3,340,645 + LRK54_RS15130 0.41 -0.9 3,340,645 + LRK54_RS15130 0.41 +0.3 3,340,645 + LRK54_RS15130 0.41 +0.1 3,340,645 + LRK54_RS15130 0.41 -1.5 3,340,645 + LRK54_RS15130 0.41 +0.4 3,340,646 - LRK54_RS15130 0.41 -0.1 3,340,646 - LRK54_RS15130 0.41 -0.8 3,340,646 - LRK54_RS15130 0.41 +0.3 3,340,646 - LRK54_RS15130 0.41 -0.8 3,340,646 - LRK54_RS15130 0.41 -0.3 3,340,646 - LRK54_RS15130 0.41 -1.0 3,340,646 - LRK54_RS15130 0.41 +1.7 3,340,646 - LRK54_RS15130 0.41 +0.3 3,340,646 - LRK54_RS15130 0.41 -1.1
Or see this region's nucleotide sequence