Experiment: R2A with Chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS12365 and LRK54_RS12370 are separated by 1 nucleotides LRK54_RS12370 and LRK54_RS12375 are separated by 95 nucleotides LRK54_RS12375 and LRK54_RS12380 are separated by 102 nucleotides
LRK54_RS12365: LRK54_RS12365 - ATP-binding protein, at 2,753,861 to 2,757,403
_RS12365
LRK54_RS12370: LRK54_RS12370 - DNA polymerase III subunit chi, at 2,757,405 to 2,757,824
_RS12370
LRK54_RS12375: LRK54_RS12375 - leucyl aminopeptidase, at 2,757,920 to 2,759,410
_RS12375
LRK54_RS12380: LRK54_RS12380 - LPS export ABC transporter permease LptF, at 2,759,513 to 2,760,634
_RS12380
Position (kb)
2757
2758
2759
2760 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.415 kb on + strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.416 kb on - strand at 2757.824 kb on + strand at 2757.824 kb on + strand at 2757.824 kb on + strand at 2757.824 kb on + strand at 2757.824 kb on + strand at 2757.824 kb on + strand at 2757.825 kb on - strand at 2757.825 kb on - strand at 2757.825 kb on - strand at 2757.825 kb on - strand at 2757.825 kb on - strand at 2757.825 kb on - strand at 2757.825 kb on - strand at 2757.825 kb on - strand at 2757.863 kb on + strand at 2757.863 kb on + strand at 2757.863 kb on + strand at 2757.863 kb on + strand at 2757.863 kb on + strand at 2757.863 kb on + strand at 2758.143 kb on + strand, within LRK54_RS12375 at 2758.143 kb on + strand, within LRK54_RS12375 at 2758.143 kb on + strand, within LRK54_RS12375 at 2758.143 kb on + strand, within LRK54_RS12375 at 2758.144 kb on - strand, within LRK54_RS12375 at 2758.144 kb on - strand, within LRK54_RS12375 at 2758.314 kb on + strand, within LRK54_RS12375 at 2758.314 kb on + strand, within LRK54_RS12375 at 2758.314 kb on + strand, within LRK54_RS12375 at 2758.314 kb on + strand, within LRK54_RS12375 at 2758.314 kb on + strand, within LRK54_RS12375 at 2758.314 kb on + strand, within LRK54_RS12375 at 2758.314 kb on + strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.315 kb on - strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.329 kb on + strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.330 kb on - strand, within LRK54_RS12375 at 2758.428 kb on + strand, within LRK54_RS12375 at 2758.428 kb on + strand, within LRK54_RS12375 at 2758.428 kb on + strand, within LRK54_RS12375 at 2758.429 kb on - strand, within LRK54_RS12375 at 2758.429 kb on - strand, within LRK54_RS12375 at 2758.629 kb on + strand, within LRK54_RS12375 at 2758.629 kb on + strand, within LRK54_RS12375 at 2758.629 kb on + strand, within LRK54_RS12375 at 2758.629 kb on + strand, within LRK54_RS12375 at 2758.629 kb on + strand, within LRK54_RS12375 at 2758.629 kb on + strand, within LRK54_RS12375 at 2758.629 kb on + strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.630 kb on - strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.659 kb on + strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.660 kb on - strand, within LRK54_RS12375 at 2758.812 kb on + strand, within LRK54_RS12375 at 2758.812 kb on + strand, within LRK54_RS12375 at 2758.812 kb on + strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.813 kb on - strand, within LRK54_RS12375 at 2758.986 kb on + strand, within LRK54_RS12375 at 2758.986 kb on + strand, within LRK54_RS12375 at 2758.987 kb on - strand, within LRK54_RS12375 at 2758.987 kb on - strand, within LRK54_RS12375 at 2759.073 kb on + strand, within LRK54_RS12375 at 2759.073 kb on + strand, within LRK54_RS12375 at 2759.073 kb on + strand, within LRK54_RS12375 at 2759.073 kb on + strand, within LRK54_RS12375 at 2759.073 kb on + strand, within LRK54_RS12375 at 2759.073 kb on + strand, within LRK54_RS12375 at 2759.074 kb on - strand, within LRK54_RS12375 at 2759.074 kb on - strand, within LRK54_RS12375 at 2759.074 kb on - strand, within LRK54_RS12375 at 2759.074 kb on - strand, within LRK54_RS12375 at 2759.139 kb on + strand, within LRK54_RS12375 at 2759.337 kb on + strand at 2759.338 kb on - strand at 2759.338 kb on - strand at 2759.338 kb on - strand at 2759.453 kb on + strand at 2759.453 kb on + strand at 2759.453 kb on + strand at 2759.453 kb on + strand at 2759.453 kb on + strand at 2759.453 kb on + strand at 2759.453 kb on + strand at 2759.453 kb on + strand at 2759.454 kb on - strand at 2759.454 kb on - strand at 2759.490 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.491 kb on + strand at 2759.507 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloride 250 mM remove 2,757,415 + +0.1 2,757,415 + -0.3 2,757,415 + +1.2 2,757,415 + -1.4 2,757,415 + -0.9 2,757,415 + -0.7 2,757,415 + +1.7 2,757,415 + -0.3 2,757,415 + -0.3 2,757,415 + +0.3 2,757,416 - -1.4 2,757,416 - -1.5 2,757,416 - +0.6 2,757,416 - -0.0 2,757,416 - -1.5 2,757,416 - -2.0 2,757,416 - +1.2 2,757,416 - +0.1 2,757,416 - -0.7 2,757,416 - +0.3 2,757,824 + +0.0 2,757,824 + +0.4 2,757,824 + +0.4 2,757,824 + -0.4 2,757,824 + -0.9 2,757,824 + +1.4 2,757,825 - -0.2 2,757,825 - -1.3 2,757,825 - +0.1 2,757,825 - +0.7 2,757,825 - +0.8 2,757,825 - -0.6 2,757,825 - -0.6 2,757,825 - -0.9 2,757,863 + -1.9 2,757,863 + +0.7 2,757,863 + +0.5 2,757,863 + -0.1 2,757,863 + -0.6 2,757,863 + +1.7 2,758,143 + LRK54_RS12375 0.15 -0.6 2,758,143 + LRK54_RS12375 0.15 -0.9 2,758,143 + LRK54_RS12375 0.15 -1.5 2,758,143 + LRK54_RS12375 0.15 -2.0 2,758,144 - LRK54_RS12375 0.15 -1.2 2,758,144 - LRK54_RS12375 0.15 -0.1 2,758,314 + LRK54_RS12375 0.26 -0.1 2,758,314 + LRK54_RS12375 0.26 -0.6 2,758,314 + LRK54_RS12375 0.26 -1.5 2,758,314 + LRK54_RS12375 0.26 +0.7 2,758,314 + LRK54_RS12375 0.26 -2.2 2,758,314 + LRK54_RS12375 0.26 +0.1 2,758,314 + LRK54_RS12375 0.26 -1.4 2,758,315 - LRK54_RS12375 0.26 +0.1 2,758,315 - LRK54_RS12375 0.26 -0.2 2,758,315 - LRK54_RS12375 0.26 +0.9 2,758,315 - LRK54_RS12375 0.26 -0.2 2,758,315 - LRK54_RS12375 0.26 +0.4 2,758,315 - LRK54_RS12375 0.26 -0.3 2,758,315 - LRK54_RS12375 0.26 -1.0 2,758,315 - LRK54_RS12375 0.26 -0.3 2,758,315 - LRK54_RS12375 0.26 -2.8 2,758,315 - LRK54_RS12375 0.26 -0.6 2,758,315 - LRK54_RS12375 0.26 +1.0 2,758,315 - LRK54_RS12375 0.26 -1.8 2,758,329 + LRK54_RS12375 0.27 -2.2 2,758,329 + LRK54_RS12375 0.27 -1.4 2,758,329 + LRK54_RS12375 0.27 -2.3 2,758,329 + LRK54_RS12375 0.27 -0.9 2,758,329 + LRK54_RS12375 0.27 -0.9 2,758,329 + LRK54_RS12375 0.27 +0.6 2,758,329 + LRK54_RS12375 0.27 -2.3 2,758,329 + LRK54_RS12375 0.27 +1.7 2,758,329 + LRK54_RS12375 0.27 -0.1 2,758,329 + LRK54_RS12375 0.27 -0.1 2,758,329 + LRK54_RS12375 0.27 -1.3 2,758,329 + LRK54_RS12375 0.27 -1.8 2,758,329 + LRK54_RS12375 0.27 -0.1 2,758,329 + LRK54_RS12375 0.27 -2.0 2,758,329 + LRK54_RS12375 0.27 -0.6 2,758,330 - LRK54_RS12375 0.27 +0.7 2,758,330 - LRK54_RS12375 0.27 -0.1 2,758,330 - LRK54_RS12375 0.27 +1.5 2,758,330 - LRK54_RS12375 0.27 -0.1 2,758,330 - LRK54_RS12375 0.27 +0.2 2,758,330 - LRK54_RS12375 0.27 -0.9 2,758,330 - LRK54_RS12375 0.27 +0.0 2,758,330 - LRK54_RS12375 0.27 -0.3 2,758,330 - LRK54_RS12375 0.27 -0.5 2,758,428 + LRK54_RS12375 0.34 +0.6 2,758,428 + LRK54_RS12375 0.34 -0.4 2,758,428 + LRK54_RS12375 0.34 -0.1 2,758,429 - LRK54_RS12375 0.34 -0.8 2,758,429 - LRK54_RS12375 0.34 +0.8 2,758,629 + LRK54_RS12375 0.48 -1.7 2,758,629 + LRK54_RS12375 0.48 +3.3 2,758,629 + LRK54_RS12375 0.48 +0.5 2,758,629 + LRK54_RS12375 0.48 -1.6 2,758,629 + LRK54_RS12375 0.48 -1.7 2,758,629 + LRK54_RS12375 0.48 -0.3 2,758,629 + LRK54_RS12375 0.48 -0.7 2,758,630 - LRK54_RS12375 0.48 -0.6 2,758,630 - LRK54_RS12375 0.48 +0.7 2,758,630 - LRK54_RS12375 0.48 -0.5 2,758,630 - LRK54_RS12375 0.48 -0.6 2,758,630 - LRK54_RS12375 0.48 +2.1 2,758,630 - LRK54_RS12375 0.48 -0.5 2,758,630 - LRK54_RS12375 0.48 +0.1 2,758,630 - LRK54_RS12375 0.48 -2.2 2,758,630 - LRK54_RS12375 0.48 +1.9 2,758,659 + LRK54_RS12375 0.50 -0.5 2,758,659 + LRK54_RS12375 0.50 -0.5 2,758,659 + LRK54_RS12375 0.50 +0.1 2,758,659 + LRK54_RS12375 0.50 -1.2 2,758,659 + LRK54_RS12375 0.50 -0.6 2,758,659 + LRK54_RS12375 0.50 -0.2 2,758,659 + LRK54_RS12375 0.50 -1.7 2,758,659 + LRK54_RS12375 0.50 -0.1 2,758,659 + LRK54_RS12375 0.50 -1.7 2,758,659 + LRK54_RS12375 0.50 -2.2 2,758,659 + LRK54_RS12375 0.50 -0.1 2,758,660 - LRK54_RS12375 0.50 -1.1 2,758,660 - LRK54_RS12375 0.50 -0.0 2,758,660 - LRK54_RS12375 0.50 -0.6 2,758,660 - LRK54_RS12375 0.50 +1.2 2,758,660 - LRK54_RS12375 0.50 -0.7 2,758,660 - LRK54_RS12375 0.50 -1.7 2,758,660 - LRK54_RS12375 0.50 -0.1 2,758,660 - LRK54_RS12375 0.50 -1.5 2,758,660 - LRK54_RS12375 0.50 -0.6 2,758,660 - LRK54_RS12375 0.50 -0.7 2,758,660 - LRK54_RS12375 0.50 -1.5 2,758,660 - LRK54_RS12375 0.50 -0.9 2,758,660 - LRK54_RS12375 0.50 -0.9 2,758,660 - LRK54_RS12375 0.50 -1.5 2,758,812 + LRK54_RS12375 0.60 -0.7 2,758,812 + LRK54_RS12375 0.60 -0.3 2,758,812 + LRK54_RS12375 0.60 -0.1 2,758,813 - LRK54_RS12375 0.60 +0.1 2,758,813 - LRK54_RS12375 0.60 -0.6 2,758,813 - LRK54_RS12375 0.60 -0.6 2,758,813 - LRK54_RS12375 0.60 -1.3 2,758,813 - LRK54_RS12375 0.60 -0.4 2,758,813 - LRK54_RS12375 0.60 -0.9 2,758,813 - LRK54_RS12375 0.60 -0.0 2,758,813 - LRK54_RS12375 0.60 -1.4 2,758,986 + LRK54_RS12375 0.71 -0.3 2,758,986 + LRK54_RS12375 0.71 -0.3 2,758,987 - LRK54_RS12375 0.72 -2.0 2,758,987 - LRK54_RS12375 0.72 -1.4 2,759,073 + LRK54_RS12375 0.77 +0.1 2,759,073 + LRK54_RS12375 0.77 -0.6 2,759,073 + LRK54_RS12375 0.77 -0.8 2,759,073 + LRK54_RS12375 0.77 -1.2 2,759,073 + LRK54_RS12375 0.77 -0.5 2,759,073 + LRK54_RS12375 0.77 -1.5 2,759,074 - LRK54_RS12375 0.77 -0.8 2,759,074 - LRK54_RS12375 0.77 -1.0 2,759,074 - LRK54_RS12375 0.77 -0.9 2,759,074 - LRK54_RS12375 0.77 -0.8 2,759,139 + LRK54_RS12375 0.82 -0.2 2,759,337 + -1.6 2,759,338 - +1.6 2,759,338 - +0.2 2,759,338 - -1.4 2,759,453 + -0.9 2,759,453 + -1.5 2,759,453 + -1.8 2,759,453 + -0.5 2,759,453 + -1.0 2,759,453 + -0.2 2,759,453 + -1.6 2,759,453 + -0.9 2,759,454 - +1.7 2,759,454 - -0.7 2,759,490 + -0.8 2,759,491 + -1.9 2,759,491 + -0.4 2,759,491 + +0.5 2,759,491 + -0.3 2,759,491 + -1.1 2,759,491 + -1.2 2,759,491 + -0.2 2,759,491 + -1.5 2,759,491 + +0.5 2,759,491 + -2.7 2,759,491 + -0.4 2,759,507 + +0.0
Or see this region's nucleotide sequence