Experiment: Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_2191 and Ga0059261_2192 are separated by 49 nucleotides Ga0059261_2192 and Ga0059261_2193 are separated by 7 nucleotides Ga0059261_2193 and Ga0059261_2194 are separated by 10 nucleotides Ga0059261_2194 and Ga0059261_2195 overlap by 4 nucleotides
Ga0059261_2191: Ga0059261_2191 - ribosomal subunit interface protein, at 2,299,292 to 2,299,870
_2191
Ga0059261_2192: Ga0059261_2192 - DNA polymerase III, epsilon subunit, Proteobacterial, at 2,299,920 to 2,300,612
_2192
Ga0059261_2193: Ga0059261_2193 - dephospho-CoA kinase, at 2,300,620 to 2,301,213
_2193
Ga0059261_2194: Ga0059261_2194 - shikimate dehydrogenase (EC 1.1.1.25) (from data) , at 2,301,224 to 2,302,036
_2194
Ga0059261_2195: Ga0059261_2195 - Nucleotide-binding protein implicated in inhibition of septum formation, at 2,302,033 to 2,302,620
_2195
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.779 kb on + strand, within Ga0059261_2191 at 2299.779 kb on + strand, within Ga0059261_2191 at 2299.779 kb on + strand, within Ga0059261_2191 at 2299.780 kb on - strand, within Ga0059261_2191 at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.871 kb on - strand at 2299.871 kb on - strand at 2299.871 kb on - strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.887 kb on - strand at 2299.887 kb on - strand at 2299.899 kb on + strand at 2299.899 kb on + strand at 2299.899 kb on + strand at 2299.899 kb on + strand at 2299.900 kb on - strand at 2299.900 kb on - strand at 2299.900 kb on - strand at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.709 kb on - strand, within Ga0059261_2194 at 2301.709 kb on - strand, within Ga0059261_2194 at 2301.727 kb on + strand, within Ga0059261_2194 at 2301.933 kb on + strand, within Ga0059261_2194 at 2301.933 kb on + strand, within Ga0059261_2194 at 2302.024 kb on - strand at 2302.024 kb on - strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.057 kb on - strand at 2302.057 kb on - strand at 2302.057 kb on - strand at 2302.057 kb on - strand at 2302.065 kb on - strand at 2302.065 kb on - strand at 2302.065 kb on - strand at 2302.065 kb on - strand at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.104 kb on - strand, within Ga0059261_2195 at 2302.104 kb on - strand, within Ga0059261_2195 at 2302.202 kb on + strand, within Ga0059261_2195 at 2302.202 kb on + strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.1 mg/ml remove 2,299,725 + Ga0059261_2191 0.75 +0.6 2,299,725 + Ga0059261_2191 0.75 -1.5 2,299,725 + Ga0059261_2191 0.75 -2.4 2,299,725 + Ga0059261_2191 0.75 -0.8 2,299,725 + Ga0059261_2191 0.75 -1.9 2,299,779 + Ga0059261_2191 0.84 -0.8 2,299,779 + Ga0059261_2191 0.84 -1.5 2,299,779 + Ga0059261_2191 0.84 -1.2 2,299,780 - Ga0059261_2191 0.84 +0.2 2,299,870 + -1.1 2,299,870 + -1.5 2,299,870 + -0.7 2,299,870 + -0.8 2,299,870 + -1.9 2,299,870 + -2.5 2,299,871 - -1.4 2,299,871 - -0.2 2,299,871 - +0.1 2,299,886 + -1.0 2,299,886 + -1.4 2,299,886 + -1.6 2,299,886 + -0.8 2,299,886 + -0.8 2,299,887 - +0.1 2,299,887 - +0.4 2,299,899 + -2.1 2,299,899 + -0.8 2,299,899 + -1.7 2,299,899 + -1.5 2,299,900 - +0.6 2,299,900 - -0.7 2,299,900 - +0.1 2,301,394 - Ga0059261_2194 0.21 -0.1 2,301,394 - Ga0059261_2194 0.21 -1.7 2,301,394 - Ga0059261_2194 0.21 +0.1 2,301,394 - Ga0059261_2194 0.21 +0.2 2,301,394 - Ga0059261_2194 0.21 +0.6 2,301,406 - Ga0059261_2194 0.22 -0.0 2,301,406 - Ga0059261_2194 0.22 -1.4 2,301,406 - Ga0059261_2194 0.22 +0.2 2,301,406 - Ga0059261_2194 0.22 -1.1 2,301,709 - Ga0059261_2194 0.60 +0.2 2,301,709 - Ga0059261_2194 0.60 +0.0 2,301,727 + Ga0059261_2194 0.62 -1.6 2,301,933 + Ga0059261_2194 0.87 +0.2 2,301,933 + Ga0059261_2194 0.87 -2.7 2,302,024 - +0.3 2,302,024 - -0.8 2,302,056 + +0.0 2,302,056 + -0.4 2,302,056 + -0.0 2,302,056 + +0.4 2,302,056 + -0.1 2,302,056 + +1.6 2,302,056 + +0.9 2,302,057 - +1.2 2,302,057 - -1.4 2,302,057 - +0.5 2,302,057 - +0.0 2,302,065 - +0.5 2,302,065 - +0.2 2,302,065 - +0.0 2,302,065 - +0.1 2,302,103 + Ga0059261_2195 0.12 -0.2 2,302,103 + Ga0059261_2195 0.12 +0.6 2,302,103 + Ga0059261_2195 0.12 -0.1 2,302,103 + Ga0059261_2195 0.12 -0.1 2,302,104 - Ga0059261_2195 0.12 -0.5 2,302,104 - Ga0059261_2195 0.12 -1.6 2,302,202 + Ga0059261_2195 0.29 -0.4 2,302,202 + Ga0059261_2195 0.29 +1.8 2,302,203 - Ga0059261_2195 0.29 +0.4 2,302,203 - Ga0059261_2195 0.29 +0.1 2,302,203 - Ga0059261_2195 0.29 -0.3 2,302,203 - Ga0059261_2195 0.29 -0.6
Or see this region's nucleotide sequence