Experiment: Polymyxin B sulfate 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_1258 and Ga0059261_1259 are separated by 57 nucleotides Ga0059261_1259 and Ga0059261_1260 overlap by 4 nucleotides Ga0059261_1260 and Ga0059261_1261 are separated by 111 nucleotides Ga0059261_1261 and Ga0059261_1262 are separated by 9 nucleotides
Ga0059261_1258: Ga0059261_1258 - TonB-dependent siderophore receptor, at 1,323,622 to 1,325,934
_1258
Ga0059261_1259: Ga0059261_1259 - Ribbon-helix-helix domain, at 1,325,992 to 1,326,270
_1259
Ga0059261_1260: Ga0059261_1260 - cobaltochelatase CobT subunit (EC 6.6.1.2), at 1,326,267 to 1,328,099
_1260
Ga0059261_1261: Ga0059261_1261 - hypothetical protein, at 1,328,211 to 1,328,768
_1261
Ga0059261_1262: Ga0059261_1262 - cobaltochelatase CobS subunit (EC 6.6.1.2), at 1,328,778 to 1,329,785
_1262
Position (kb)
1326
1327
1328
1329 Strain fitness (log2 ratio)
-1
0
1
2 at 1325.364 kb on - strand, within Ga0059261_1258 at 1325.366 kb on + strand, within Ga0059261_1258 at 1325.367 kb on - strand, within Ga0059261_1258 at 1325.367 kb on - strand at 1325.367 kb on - strand, within Ga0059261_1258 at 1325.367 kb on - strand, within Ga0059261_1258 at 1325.468 kb on + strand, within Ga0059261_1258 at 1325.468 kb on + strand, within Ga0059261_1258 at 1325.468 kb on + strand, within Ga0059261_1258 at 1325.469 kb on - strand, within Ga0059261_1258 at 1325.469 kb on - strand at 1325.469 kb on - strand, within Ga0059261_1258 at 1325.469 kb on - strand, within Ga0059261_1258 at 1325.469 kb on - strand, within Ga0059261_1258 at 1325.469 kb on - strand, within Ga0059261_1258 at 1325.532 kb on - strand, within Ga0059261_1258 at 1325.954 kb on + strand at 1325.954 kb on + strand at 1325.954 kb on + strand at 1325.954 kb on + strand at 1326.187 kb on + strand, within Ga0059261_1259 at 1326.187 kb on + strand, within Ga0059261_1259 at 1326.187 kb on + strand, within Ga0059261_1259 at 1326.187 kb on + strand, within Ga0059261_1259 at 1326.187 kb on + strand, within Ga0059261_1259 at 1326.188 kb on - strand, within Ga0059261_1259 at 1326.188 kb on - strand, within Ga0059261_1259 at 1326.355 kb on + strand at 1326.355 kb on + strand at 1326.355 kb on + strand at 1326.355 kb on + strand at 1326.356 kb on - strand at 1326.356 kb on - strand at 1326.356 kb on - strand at 1326.358 kb on + strand at 1326.358 kb on + strand at 1326.359 kb on - strand at 1326.454 kb on + strand, within Ga0059261_1260 at 1326.454 kb on + strand, within Ga0059261_1260 at 1326.455 kb on - strand, within Ga0059261_1260 at 1326.455 kb on - strand, within Ga0059261_1260 at 1326.664 kb on + strand, within Ga0059261_1260 at 1326.664 kb on + strand, within Ga0059261_1260 at 1326.665 kb on + strand, within Ga0059261_1260 at 1326.665 kb on - strand, within Ga0059261_1260 at 1326.928 kb on + strand, within Ga0059261_1260 at 1326.928 kb on + strand, within Ga0059261_1260 at 1326.928 kb on + strand, within Ga0059261_1260 at 1326.929 kb on - strand, within Ga0059261_1260 at 1326.929 kb on - strand, within Ga0059261_1260 at 1326.929 kb on - strand, within Ga0059261_1260 at 1326.929 kb on - strand, within Ga0059261_1260 at 1327.102 kb on + strand, within Ga0059261_1260 at 1327.102 kb on + strand, within Ga0059261_1260 at 1327.165 kb on + strand, within Ga0059261_1260 at 1327.165 kb on + strand, within Ga0059261_1260 at 1327.165 kb on + strand, within Ga0059261_1260 at 1327.165 kb on + strand, within Ga0059261_1260 at 1327.165 kb on + strand, within Ga0059261_1260 at 1327.166 kb on - strand, within Ga0059261_1260 at 1327.166 kb on - strand, within Ga0059261_1260 at 1327.166 kb on - strand, within Ga0059261_1260 at 1327.184 kb on + strand, within Ga0059261_1260 at 1327.184 kb on + strand, within Ga0059261_1260 at 1327.184 kb on + strand, within Ga0059261_1260 at 1327.185 kb on - strand, within Ga0059261_1260 at 1327.185 kb on - strand, within Ga0059261_1260 at 1327.185 kb on - strand, within Ga0059261_1260 at 1327.185 kb on - strand, within Ga0059261_1260 at 1327.187 kb on - strand, within Ga0059261_1260 at 1327.187 kb on - strand, within Ga0059261_1260 at 1327.774 kb on + strand, within Ga0059261_1260 at 1327.775 kb on - strand, within Ga0059261_1260 at 1328.058 kb on - strand at 1328.065 kb on + strand at 1328.126 kb on + strand at 1328.182 kb on - strand at 1328.523 kb on + strand, within Ga0059261_1261 at 1328.524 kb on - strand, within Ga0059261_1261 at 1328.524 kb on - strand, within Ga0059261_1261 at 1328.833 kb on + strand at 1328.833 kb on + strand at 1328.834 kb on - strand at 1328.834 kb on - strand at 1328.836 kb on + strand at 1328.836 kb on + strand at 1328.837 kb on - strand at 1328.837 kb on - strand at 1328.837 kb on - strand at 1328.837 kb on - strand at 1328.924 kb on + strand, within Ga0059261_1262 at 1329.056 kb on - strand, within Ga0059261_1262
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.1 mg/ml remove 1,325,364 - Ga0059261_1258 0.75 -0.7 1,325,366 + Ga0059261_1258 0.75 -0.1 1,325,367 - Ga0059261_1258 0.75 -0.6 1,325,367 - -1.0 1,325,367 - Ga0059261_1258 0.75 -0.8 1,325,367 - Ga0059261_1258 0.75 +0.0 1,325,468 + Ga0059261_1258 0.80 +0.3 1,325,468 + Ga0059261_1258 0.80 -0.7 1,325,468 + Ga0059261_1258 0.80 -0.8 1,325,469 - Ga0059261_1258 0.80 -0.2 1,325,469 - +1.0 1,325,469 - Ga0059261_1258 0.80 -0.3 1,325,469 - Ga0059261_1258 0.80 -1.2 1,325,469 - Ga0059261_1258 0.80 +0.2 1,325,469 - Ga0059261_1258 0.80 -0.4 1,325,532 - Ga0059261_1258 0.83 -1.0 1,325,954 + -0.9 1,325,954 + +0.0 1,325,954 + +0.4 1,325,954 + -0.6 1,326,187 + Ga0059261_1259 0.70 +0.5 1,326,187 + Ga0059261_1259 0.70 -0.4 1,326,187 + Ga0059261_1259 0.70 -0.2 1,326,187 + Ga0059261_1259 0.70 +0.3 1,326,187 + Ga0059261_1259 0.70 -0.2 1,326,188 - Ga0059261_1259 0.70 -0.5 1,326,188 - Ga0059261_1259 0.70 -0.3 1,326,355 + -0.3 1,326,355 + -1.2 1,326,355 + +0.2 1,326,355 + +0.2 1,326,356 - +0.4 1,326,356 - -1.0 1,326,356 - +0.3 1,326,358 + -1.6 1,326,358 + -0.7 1,326,359 - -0.1 1,326,454 + Ga0059261_1260 0.10 +0.3 1,326,454 + Ga0059261_1260 0.10 +0.9 1,326,455 - Ga0059261_1260 0.10 +0.2 1,326,455 - Ga0059261_1260 0.10 +1.0 1,326,664 + Ga0059261_1260 0.22 -0.0 1,326,664 + Ga0059261_1260 0.22 +1.5 1,326,665 + Ga0059261_1260 0.22 +0.9 1,326,665 - Ga0059261_1260 0.22 +0.5 1,326,928 + Ga0059261_1260 0.36 +0.4 1,326,928 + Ga0059261_1260 0.36 +1.2 1,326,928 + Ga0059261_1260 0.36 -0.6 1,326,929 - Ga0059261_1260 0.36 +0.8 1,326,929 - Ga0059261_1260 0.36 +1.0 1,326,929 - Ga0059261_1260 0.36 +0.4 1,326,929 - Ga0059261_1260 0.36 +0.4 1,327,102 + Ga0059261_1260 0.46 +0.5 1,327,102 + Ga0059261_1260 0.46 +2.0 1,327,165 + Ga0059261_1260 0.49 +0.0 1,327,165 + Ga0059261_1260 0.49 +0.7 1,327,165 + Ga0059261_1260 0.49 +1.7 1,327,165 + Ga0059261_1260 0.49 +1.0 1,327,165 + Ga0059261_1260 0.49 +1.2 1,327,166 - Ga0059261_1260 0.49 +0.5 1,327,166 - Ga0059261_1260 0.49 +1.2 1,327,166 - Ga0059261_1260 0.49 +1.2 1,327,184 + Ga0059261_1260 0.50 +0.1 1,327,184 + Ga0059261_1260 0.50 +0.7 1,327,184 + Ga0059261_1260 0.50 +1.3 1,327,185 - Ga0059261_1260 0.50 +1.0 1,327,185 - Ga0059261_1260 0.50 +2.6 1,327,185 - Ga0059261_1260 0.50 +0.8 1,327,185 - Ga0059261_1260 0.50 -0.1 1,327,187 - Ga0059261_1260 0.50 +0.1 1,327,187 - Ga0059261_1260 0.50 -1.0 1,327,774 + Ga0059261_1260 0.82 +1.4 1,327,775 - Ga0059261_1260 0.82 +0.6 1,328,058 - +0.9 1,328,065 + +1.2 1,328,126 + +0.5 1,328,182 - +0.2 1,328,523 + Ga0059261_1261 0.56 +1.4 1,328,524 - Ga0059261_1261 0.56 -1.0 1,328,524 - Ga0059261_1261 0.56 +0.2 1,328,833 + +0.8 1,328,833 + +0.2 1,328,834 - +1.1 1,328,834 - +0.2 1,328,836 + +1.3 1,328,836 + -0.4 1,328,837 - +1.0 1,328,837 - +1.2 1,328,837 - +0.4 1,328,837 - +0.9 1,328,924 + Ga0059261_1262 0.14 +0.9 1,329,056 - Ga0059261_1262 0.28 +1.7
Or see this region's nucleotide sequence