Experiment: MoYLS4 with Neomycin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0380 and DvMF_0381 are separated by 466 nucleotides DvMF_0381 and DvMF_0382 are separated by 391 nucleotides
DvMF_0380: DvMF_0380 - hypothetical protein (RefSeq), at 447,301 to 447,816
_0380
DvMF_0381: DvMF_0381 - L-lactate transport (RefSeq), at 448,283 to 449,803
_0381
DvMF_0382: DvMF_0382 - phosphate uptake regulator, PhoU (RefSeq), at 450,195 to 450,878
_0382
Position (kb) 448
449
450 Strain fitness (log2 ratio) -2
-1
0
1 at 447.305 kb on - strand at 447.359 kb on + strand, within DvMF_0380 at 447.432 kb on + strand, within DvMF_0380 at 447.440 kb on - strand, within DvMF_0380 at 447.440 kb on - strand, within DvMF_0380 at 447.440 kb on - strand, within DvMF_0380 at 447.440 kb on - strand, within DvMF_0380 at 447.440 kb on - strand, within DvMF_0380 at 448.041 kb on - strand at 448.073 kb on + strand at 448.099 kb on - strand at 448.129 kb on - strand at 448.287 kb on + strand at 448.674 kb on + strand, within DvMF_0381 at 448.676 kb on - strand, within DvMF_0381 at 448.886 kb on + strand, within DvMF_0381 at 449.000 kb on - strand, within DvMF_0381 at 449.106 kb on + strand, within DvMF_0381 at 449.342 kb on - strand, within DvMF_0381 at 449.551 kb on + strand, within DvMF_0381 at 449.682 kb on + strand at 449.739 kb on - strand at 450.264 kb on + strand, within DvMF_0382 at 450.373 kb on + strand, within DvMF_0382 at 450.381 kb on - strand, within DvMF_0382 at 450.388 kb on - strand, within DvMF_0382 at 450.389 kb on + strand, within DvMF_0382 at 450.516 kb on - strand, within DvMF_0382 at 450.624 kb on + strand, within DvMF_0382 at 450.644 kb on - strand, within DvMF_0382
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Neomycin 0.125 mg/ml remove 447,305 - -0.9 447,359 + DvMF_0380 0.11 -0.6 447,432 + DvMF_0380 0.25 +0.3 447,440 - DvMF_0380 0.27 -0.6 447,440 - DvMF_0380 0.27 +0.6 447,440 - DvMF_0380 0.27 -0.4 447,440 - DvMF_0380 0.27 -0.4 447,440 - DvMF_0380 0.27 -0.1 448,041 - -0.8 448,073 + -0.8 448,099 - +0.2 448,129 - -1.1 448,287 + -0.3 448,674 + DvMF_0381 0.26 -1.9 448,676 - DvMF_0381 0.26 -0.0 448,886 + DvMF_0381 0.40 -2.5 449,000 - DvMF_0381 0.47 -0.8 449,106 + DvMF_0381 0.54 -1.4 449,342 - DvMF_0381 0.70 -1.8 449,551 + DvMF_0381 0.83 +0.6 449,682 + -1.4 449,739 - -0.7 450,264 + DvMF_0382 0.10 -0.8 450,373 + DvMF_0382 0.26 +0.1 450,381 - DvMF_0382 0.27 +0.4 450,388 - DvMF_0382 0.28 -0.3 450,389 + DvMF_0382 0.28 -0.5 450,516 - DvMF_0382 0.47 +0.1 450,624 + DvMF_0382 0.63 -1.4 450,644 - DvMF_0382 0.66 -0.1
Or see this region's nucleotide sequence