Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3704 and Shew_3705 are separated by 28 nucleotides Shew_3705 and Shew_3706 are separated by 65 nucleotides Shew_3706 and Shew_3707 are separated by 27 nucleotides
Shew_3704: Shew_3704 - dTDP-glucose 4,6-dehydratase (RefSeq), at 4,408,268 to 4,409,338
_3704
Shew_3705: Shew_3705 - dTDP-4-dehydrorhamnose reductase (RefSeq), at 4,409,367 to 4,410,296
_3705
Shew_3706: Shew_3706 - 3-deoxy-D-manno-octulosonic-acid kinase (RefSeq), at 4,410,362 to 4,411,087
_3706
Shew_3707: Shew_3707 - 3-deoxy-D-manno-octulosonic-acid transferase domain-containing protein (RefSeq), at 4,411,115 to 4,412,380
_3707
Position (kb) 4409
4410
4411 Strain fitness (log2 ratio) -1
0
1 at 4408.390 kb on - strand, within Shew_3704 at 4408.392 kb on + strand, within Shew_3704 at 4408.493 kb on - strand, within Shew_3704 at 4408.611 kb on + strand, within Shew_3704 at 4408.640 kb on - strand, within Shew_3704 at 4408.915 kb on + strand, within Shew_3704 at 4408.985 kb on - strand, within Shew_3704 at 4408.998 kb on + strand, within Shew_3704 at 4409.006 kb on - strand, within Shew_3704 at 4409.422 kb on - strand at 4409.630 kb on - strand, within Shew_3705 at 4409.936 kb on + strand, within Shew_3705 at 4409.992 kb on + strand, within Shew_3705 at 4411.081 kb on + strand at 4411.089 kb on - strand at 4411.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 4,408,390 - Shew_3704 0.11 -1.1 4,408,392 + Shew_3704 0.12 -0.7 4,408,493 - Shew_3704 0.21 -1.0 4,408,611 + Shew_3704 0.32 -0.7 4,408,640 - Shew_3704 0.35 +0.6 4,408,915 + Shew_3704 0.60 +0.1 4,408,985 - Shew_3704 0.67 +0.2 4,408,998 + Shew_3704 0.68 -0.1 4,409,006 - Shew_3704 0.69 +0.1 4,409,422 - -0.1 4,409,630 - Shew_3705 0.28 +0.4 4,409,936 + Shew_3705 0.61 +0.0 4,409,992 + Shew_3705 0.67 +0.9 4,411,081 + +0.5 4,411,089 - +0.1 4,411,089 - -0.1
Or see this region's nucleotide sequence