Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3630 and Shew_3631 are separated by 58 nucleotides Shew_3631 and Shew_3632 overlap by 8 nucleotides Shew_3632 and Shew_3633 are separated by 492 nucleotides Shew_3633 and Shew_3634 are separated by 87 nucleotides
Shew_3630: Shew_3630 - bioH protein (RefSeq), at 4,328,079 to 4,328,858
_3630
Shew_3631: Shew_3631 - hypothetical protein (RefSeq), at 4,328,917 to 4,329,411
_3631
Shew_3632: Shew_3632 - sulfatase (RefSeq), at 4,329,404 to 4,331,392
_3632
Shew_3633: Shew_3633 - hypothetical protein (RefSeq), at 4,331,885 to 4,332,103
_3633
Shew_3634: Shew_3634 - RNA-binding S1 domain-containing protein (RefSeq), at 4,332,191 to 4,334,554
_3634
Position (kb) 4329
4330
4331
4332 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 4328.570 kb on - strand, within Shew_3630 at 4328.834 kb on - strand at 4329.085 kb on - strand, within Shew_3631 at 4329.289 kb on + strand, within Shew_3631 at 4329.434 kb on + strand at 4329.457 kb on - strand at 4329.548 kb on + strand at 4329.751 kb on - strand, within Shew_3632 at 4329.762 kb on - strand, within Shew_3632 at 4329.838 kb on + strand, within Shew_3632 at 4329.850 kb on + strand, within Shew_3632 at 4330.063 kb on - strand, within Shew_3632 at 4330.089 kb on + strand, within Shew_3632 at 4330.224 kb on - strand, within Shew_3632 at 4330.522 kb on - strand, within Shew_3632 at 4330.522 kb on - strand, within Shew_3632 at 4330.672 kb on + strand, within Shew_3632 at 4330.680 kb on - strand, within Shew_3632 at 4330.815 kb on - strand, within Shew_3632 at 4330.956 kb on + strand, within Shew_3632 at 4331.175 kb on + strand, within Shew_3632 at 4331.743 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 4,328,570 - Shew_3630 0.63 -1.0 4,328,834 - -3.0 4,329,085 - Shew_3631 0.34 +0.3 4,329,289 + Shew_3631 0.75 -0.7 4,329,434 + -1.1 4,329,457 - -3.2 4,329,548 + -3.1 4,329,751 - Shew_3632 0.17 -3.2 4,329,762 - Shew_3632 0.18 -3.3 4,329,838 + Shew_3632 0.22 -4.3 4,329,850 + Shew_3632 0.22 -5.1 4,330,063 - Shew_3632 0.33 -4.6 4,330,089 + Shew_3632 0.34 -6.1 4,330,224 - Shew_3632 0.41 -4.4 4,330,522 - Shew_3632 0.56 -3.9 4,330,522 - Shew_3632 0.56 -3.8 4,330,672 + Shew_3632 0.64 -3.9 4,330,680 - Shew_3632 0.64 -4.2 4,330,815 - Shew_3632 0.71 -6.0 4,330,956 + Shew_3632 0.78 -3.3 4,331,175 + Shew_3632 0.89 -4.3 4,331,743 + +0.6
Or see this region's nucleotide sequence