Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2883 and Shew_2884 are separated by 125 nucleotides Shew_2884 and Shew_2885 are separated by 129 nucleotides Shew_2885 and Shew_2886 are separated by 110 nucleotides
Shew_2883: Shew_2883 - BolA family protein (RefSeq), at 3,423,038 to 3,423,337
_2883
Shew_2884: Shew_2884 - glycine cleavage system regulatory protein-like protein (RefSeq), at 3,423,463 to 3,423,966
_2884
Shew_2885: Shew_2885 - 2OG-Fe(II) oxygenase (RefSeq), at 3,424,096 to 3,424,743
_2885
Shew_2886: Shew_2886 - rRNA (guanine-N(2)-)-methyltransferase (RefSeq), at 3,424,854 to 3,426,059
_2886
Position (kb)
3424
3425 Strain fitness (log2 ratio)
-2
-1
0
1 at 3423.217 kb on + strand, within Shew_2883 at 3423.469 kb on - strand at 3423.497 kb on + strand at 3423.666 kb on + strand, within Shew_2884 at 3423.675 kb on + strand, within Shew_2884 at 3423.918 kb on + strand at 3424.063 kb on - strand at 3424.073 kb on - strand at 3424.073 kb on - strand at 3424.160 kb on - strand at 3424.247 kb on + strand, within Shew_2885 at 3424.247 kb on + strand, within Shew_2885 at 3424.258 kb on + strand, within Shew_2885 at 3424.271 kb on - strand, within Shew_2885 at 3424.317 kb on - strand, within Shew_2885 at 3424.457 kb on - strand, within Shew_2885 at 3424.457 kb on - strand, within Shew_2885 at 3424.493 kb on - strand, within Shew_2885 at 3424.519 kb on + strand, within Shew_2885 at 3424.650 kb on + strand, within Shew_2885 at 3424.796 kb on - strand at 3424.858 kb on - strand at 3424.997 kb on - strand, within Shew_2886 at 3425.193 kb on - strand, within Shew_2886 at 3425.193 kb on - strand, within Shew_2886 at 3425.329 kb on - strand, within Shew_2886 at 3425.452 kb on + strand, within Shew_2886 at 3425.533 kb on - strand, within Shew_2886
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 3,423,217 + Shew_2883 0.60 -0.3 3,423,469 - -0.1 3,423,497 + +0.5 3,423,666 + Shew_2884 0.40 +0.2 3,423,675 + Shew_2884 0.42 -0.1 3,423,918 + -0.2 3,424,063 - +0.6 3,424,073 - +0.3 3,424,073 - +0.1 3,424,160 - -0.1 3,424,247 + Shew_2885 0.23 -0.2 3,424,247 + Shew_2885 0.23 -0.1 3,424,258 + Shew_2885 0.25 -0.3 3,424,271 - Shew_2885 0.27 -0.1 3,424,317 - Shew_2885 0.34 -0.1 3,424,457 - Shew_2885 0.56 -0.9 3,424,457 - Shew_2885 0.56 -0.4 3,424,493 - Shew_2885 0.61 -0.1 3,424,519 + Shew_2885 0.65 +0.5 3,424,650 + Shew_2885 0.85 +0.5 3,424,796 - -0.0 3,424,858 - -0.7 3,424,997 - Shew_2886 0.12 -0.5 3,425,193 - Shew_2886 0.28 +0.1 3,425,193 - Shew_2886 0.28 -0.2 3,425,329 - Shew_2886 0.39 -2.4 3,425,452 + Shew_2886 0.50 -0.7 3,425,533 - Shew_2886 0.56 -0.4
Or see this region's nucleotide sequence