Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2534 and Shew_2535 are separated by 226 nucleotides Shew_2535 and Shew_2536 are separated by 40 nucleotides Shew_2536 and Shew_2537 are separated by 307 nucleotides
Shew_2534: Shew_2534 - glycerate kinase (RefSeq), at 2,991,493 to 2,992,638
_2534
Shew_2535: Shew_2535 - pyridoxal-dependent decarboxylase (RefSeq), at 2,992,865 to 2,994,505
_2535
Shew_2536: Shew_2536 - putative thiol-disulphide oxidoreductase DCC (RefSeq), at 2,994,546 to 2,995,016
_2536
Shew_2537: Shew_2537 - AraC family transcriptional regulator (RefSeq), at 2,995,324 to 2,996,262
_2537
Position (kb)
2992
2993
2994
2995 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2991.875 kb on - strand, within Shew_2534 at 2992.336 kb on - strand, within Shew_2534 at 2992.336 kb on - strand, within Shew_2534 at 2992.402 kb on - strand, within Shew_2534 at 2992.402 kb on - strand, within Shew_2534 at 2992.649 kb on + strand at 2992.785 kb on - strand at 2993.619 kb on + strand, within Shew_2535 at 2994.378 kb on + strand at 2994.655 kb on + strand, within Shew_2536 at 2994.737 kb on + strand, within Shew_2536 at 2994.958 kb on + strand, within Shew_2536 at 2995.031 kb on + strand at 2995.127 kb on - strand at 2995.157 kb on + strand at 2995.435 kb on - strand, within Shew_2537
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 2,991,875 - Shew_2534 0.33 -0.1 2,992,336 - Shew_2534 0.74 -4.0 2,992,336 - Shew_2534 0.74 -0.8 2,992,402 - Shew_2534 0.79 +0.4 2,992,402 - Shew_2534 0.79 -0.1 2,992,649 + +0.1 2,992,785 - +2.5 2,993,619 + Shew_2535 0.46 +2.9 2,994,378 + +1.2 2,994,655 + Shew_2536 0.23 +0.4 2,994,737 + Shew_2536 0.41 -1.8 2,994,958 + Shew_2536 0.87 -0.9 2,995,031 + -0.5 2,995,127 - +0.9 2,995,157 + +0.4 2,995,435 - Shew_2537 0.12 +0.2
Or see this region's nucleotide sequence