Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspB and Shew_2496 are separated by 20 nucleotides Shew_2496 and Shew_2497 are separated by 177 nucleotides Shew_2497 and Shew_2498 are separated by 87 nucleotides
Shew_2495: pspB - phage shock protein B (RefSeq), at 2,934,975 to 2,935,214
pspB
Shew_2496: Shew_2496 - phage shock protein A, PspA (RefSeq), at 2,935,235 to 2,935,918
_2496
Shew_2497: Shew_2497 - sigma-54 dependent trancsriptional regulator (RefSeq), at 2,936,096 to 2,937,166
_2497
Shew_2498: Shew_2498 - extracellular solute-binding protein (RefSeq), at 2,937,254 to 2,938,897
_2498
Position (kb)
2936
2937
2938 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2935.299 kb on - strand at 2935.360 kb on + strand, within Shew_2496 at 2935.611 kb on - strand, within Shew_2496 at 2935.611 kb on - strand, within Shew_2496 at 2935.682 kb on - strand, within Shew_2496 at 2935.953 kb on + strand at 2936.122 kb on + strand at 2936.130 kb on - strand at 2936.130 kb on - strand at 2936.142 kb on - strand at 2936.142 kb on - strand at 2936.249 kb on + strand, within Shew_2497 at 2936.436 kb on + strand, within Shew_2497 at 2936.436 kb on + strand, within Shew_2497 at 2936.444 kb on - strand, within Shew_2497 at 2936.517 kb on + strand, within Shew_2497 at 2936.810 kb on + strand, within Shew_2497 at 2936.961 kb on + strand, within Shew_2497 at 2937.207 kb on - strand at 2937.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 2,935,299 - +0.9 2,935,360 + Shew_2496 0.18 +0.6 2,935,611 - Shew_2496 0.55 -0.1 2,935,611 - Shew_2496 0.55 -2.3 2,935,682 - Shew_2496 0.65 -0.7 2,935,953 + +0.3 2,936,122 + +0.2 2,936,130 - +0.4 2,936,130 - +0.0 2,936,142 - -0.1 2,936,142 - +0.3 2,936,249 + Shew_2497 0.14 +0.1 2,936,436 + Shew_2497 0.32 -1.5 2,936,436 + Shew_2497 0.32 +0.7 2,936,444 - Shew_2497 0.32 -1.1 2,936,517 + Shew_2497 0.39 -2.6 2,936,810 + Shew_2497 0.67 -0.1 2,936,961 + Shew_2497 0.81 -0.2 2,937,207 - +3.4 2,937,282 - +3.2
Or see this region's nucleotide sequence