Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2283 and Shew_2284 are separated by 288 nucleotides Shew_2284 and Shew_2285 are separated by 31 nucleotides Shew_2285 and Shew_2286 overlap by 17 nucleotides
Shew_2283: Shew_2283 - porin (RefSeq), at 2,667,624 to 2,668,781
_2283
Shew_2284: Shew_2284 - hypothetical protein (RefSeq), at 2,669,070 to 2,669,291
_2284
Shew_2285: Shew_2285 - UDP-glucose 4-epimerase (RefSeq), at 2,669,323 to 2,670,378
_2285
Shew_2286: Shew_2286 - SSS family solute/sodium (Na+) symporter (RefSeq), at 2,670,362 to 2,671,921
_2286
Position (kb)
2669
2670
2671 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2668.638 kb on + strand, within Shew_2283 at 2668.638 kb on + strand, within Shew_2283 at 2668.737 kb on + strand at 2669.009 kb on + strand at 2669.010 kb on - strand at 2669.067 kb on - strand at 2669.156 kb on + strand, within Shew_2284 at 2669.164 kb on - strand, within Shew_2284 at 2669.170 kb on + strand, within Shew_2284 at 2669.179 kb on + strand, within Shew_2284 at 2669.365 kb on + strand at 2669.799 kb on + strand, within Shew_2285 at 2669.848 kb on - strand, within Shew_2285 at 2670.041 kb on - strand, within Shew_2285 at 2670.522 kb on - strand, within Shew_2286 at 2670.541 kb on - strand, within Shew_2286
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 2,668,638 + Shew_2283 0.88 -0.5 2,668,638 + Shew_2283 0.88 +0.1 2,668,737 + -0.4 2,669,009 + +0.2 2,669,010 - +0.1 2,669,067 - -0.5 2,669,156 + Shew_2284 0.39 +0.3 2,669,164 - Shew_2284 0.42 -0.1 2,669,170 + Shew_2284 0.45 -0.6 2,669,179 + Shew_2284 0.49 -0.2 2,669,365 + +0.0 2,669,799 + Shew_2285 0.45 +0.2 2,669,848 - Shew_2285 0.50 +3.7 2,670,041 - Shew_2285 0.68 -0.1 2,670,522 - Shew_2286 0.10 +0.6 2,670,541 - Shew_2286 0.11 -0.2
Or see this region's nucleotide sequence