Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2115 and Shew_2116 are separated by 217 nucleotides Shew_2116 and Shew_2117 are separated by 37 nucleotides Shew_2117 and Shew_2118 are separated by 209 nucleotides
Shew_2115: Shew_2115 - lytic transglycosylase, catalytic (RefSeq), at 2,450,005 to 2,451,540
_2115
Shew_2116: Shew_2116 - AsmA family protein (RefSeq), at 2,451,758 to 2,453,602
_2116
Shew_2117: Shew_2117 - ribonuclease activity regulator protein RraA (RefSeq), at 2,453,640 to 2,454,134
_2117
Shew_2118: Shew_2118 - hypothetical protein (RefSeq), at 2,454,344 to 2,454,658
_2118
Position (kb)
2451
2452
2453
2454 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2450.780 kb on - strand, within Shew_2115 at 2451.108 kb on - strand, within Shew_2115 at 2451.376 kb on - strand, within Shew_2115 at 2451.560 kb on + strand at 2452.011 kb on - strand, within Shew_2116 at 2452.092 kb on - strand, within Shew_2116 at 2452.440 kb on + strand, within Shew_2116 at 2452.758 kb on + strand, within Shew_2116 at 2453.209 kb on - strand, within Shew_2116 at 2453.280 kb on + strand, within Shew_2116 at 2453.388 kb on - strand, within Shew_2116 at 2453.434 kb on - strand at 2453.529 kb on - strand at 2453.910 kb on + strand, within Shew_2117 at 2454.153 kb on - strand at 2454.474 kb on + strand, within Shew_2118 at 2454.533 kb on + strand, within Shew_2118 at 2454.571 kb on - strand, within Shew_2118
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 2,450,780 - Shew_2115 0.50 +0.1 2,451,108 - Shew_2115 0.72 -1.5 2,451,376 - Shew_2115 0.89 -0.4 2,451,560 + +0.1 2,452,011 - Shew_2116 0.14 -3.3 2,452,092 - Shew_2116 0.18 +0.9 2,452,440 + Shew_2116 0.37 +0.8 2,452,758 + Shew_2116 0.54 -2.1 2,453,209 - Shew_2116 0.79 -0.8 2,453,280 + Shew_2116 0.82 -1.7 2,453,388 - Shew_2116 0.88 -1.8 2,453,434 - -0.9 2,453,529 - -0.4 2,453,910 + Shew_2117 0.55 +0.0 2,454,153 - +0.3 2,454,474 + Shew_2118 0.41 -0.9 2,454,533 + Shew_2118 0.60 -0.4 2,454,571 - Shew_2118 0.72 -0.2
Or see this region's nucleotide sequence