Strain Fitness in Shewanella loihica PV-4 around Shew_2075

Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntruvB and Shew_2076 are separated by 19 nucleotidesShew_2076 and Shew_2077 overlap by 4 nucleotides Shew_2075: ruvB - Holliday junction DNA helicase B (RefSeq), at 2,399,610 to 2,400,623 ruvB Shew_2076: Shew_2076 - Holliday junction DNA helicase RuvA (RefSeq), at 2,400,643 to 2,401,260 _2076 Shew_2077: Shew_2077 - crossover junction endodeoxyribonuclease RuvC (RefSeq), at 2,401,257 to 2,401,778 _2077 Position (kb) 2399 2400 2401Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2399.479 kb on + strandat 2399.484 kb on + strandat 2399.674 kb on - strandat 2399.674 kb on - strandat 2399.681 kb on + strandat 2399.736 kb on - strand, within ruvBat 2400.175 kb on - strand, within ruvBat 2400.409 kb on + strand, within ruvBat 2400.409 kb on + strand, within ruvBat 2401.337 kb on + strand, within Shew_2077at 2401.407 kb on + strand, within Shew_2077

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM
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2,399,479 + -2.1
2,399,484 + -0.4
2,399,674 - -4.1
2,399,674 - -4.4
2,399,681 + -2.9
2,399,736 - ruvB Shew_2075 0.12 -3.0
2,400,175 - ruvB Shew_2075 0.56 -4.8
2,400,409 + ruvB Shew_2075 0.79 -3.1
2,400,409 + ruvB Shew_2075 0.79 -3.2
2,401,337 + Shew_2077 0.15 -1.5
2,401,407 + Shew_2077 0.29 -2.7

Or see this region's nucleotide sequence