Strain Fitness in Shewanella loihica PV-4 around Shew_1933

Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcls and Shew_1933 are separated by 80 nucleotidesShew_1933 and Shew_1934 are separated by 46 nucleotides Shew_1932: cls - cardiolipin synthetase (RefSeq), at 2,231,216 to 2,232,814 cls Shew_1933: Shew_1933 - endonuclease/exonuclease/phosphatase (RefSeq), at 2,232,895 to 2,233,773 _1933 Shew_1934: Shew_1934 - hypothetical protein (RefSeq), at 2,233,820 to 2,234,548 _1934 Position (kb) 2232 2233 2234Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 2232.310 kb on - strand, within clsat 2232.885 kb on + strandat 2233.249 kb on - strand, within Shew_1933at 2233.426 kb on - strand, within Shew_1933at 2234.303 kb on + strand, within Shew_1934at 2234.311 kb on - strand, within Shew_1934at 2234.552 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM
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2,232,310 - cls Shew_1932 0.68 -1.2
2,232,885 + -0.2
2,233,249 - Shew_1933 0.40 -4.0
2,233,426 - Shew_1933 0.60 -5.0
2,234,303 + Shew_1934 0.66 +0.4
2,234,311 - Shew_1934 0.67 +0.2
2,234,552 - +0.3

Or see this region's nucleotide sequence