Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1844 and Shew_1845 are separated by 113 nucleotides Shew_1845 and Shew_1846 are separated by 101 nucleotides Shew_1846 and Shew_1847 overlap by 4 nucleotides
Shew_1844: Shew_1844 - chromosome replication initiation inhibitor protein (RefSeq), at 2,120,304 to 2,121,191
_1844
Shew_1845: Shew_1845 - lysine exporter protein LysE/YggA (RefSeq), at 2,121,305 to 2,121,949
_1845
Shew_1846: Shew_1846 - redoxin domain-containing protein (RefSeq), at 2,122,051 to 2,122,521
_1846
Shew_1847: Shew_1847 - amino acid-binding ACT domain-containing protein (RefSeq), at 2,122,518 to 2,123,072
_1847
Position (kb)
2121
2122 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2120.653 kb on - strand, within Shew_1844 at 2120.687 kb on - strand, within Shew_1844 at 2120.766 kb on + strand, within Shew_1844 at 2120.774 kb on - strand, within Shew_1844 at 2120.800 kb on - strand, within Shew_1844 at 2120.808 kb on - strand, within Shew_1844 at 2121.220 kb on + strand at 2121.220 kb on + strand at 2121.573 kb on - strand, within Shew_1845 at 2121.682 kb on + strand, within Shew_1845 at 2121.690 kb on - strand, within Shew_1845 at 2121.924 kb on - strand at 2121.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM remove 2,120,653 - Shew_1844 0.39 -0.1 2,120,687 - Shew_1844 0.43 -0.9 2,120,766 + Shew_1844 0.52 -0.4 2,120,774 - Shew_1844 0.53 -0.4 2,120,800 - Shew_1844 0.56 -3.5 2,120,808 - Shew_1844 0.57 -2.1 2,121,220 + -0.4 2,121,220 + -0.7 2,121,573 - Shew_1845 0.42 -1.4 2,121,682 + Shew_1845 0.58 -1.5 2,121,690 - Shew_1845 0.60 -4.1 2,121,924 - -1.2 2,121,935 - -1.3
Or see this region's nucleotide sequence