Strain Fitness in Shewanella loihica PV-4 around Shew_1514

Experiment: 1-ethyl-3-methylimidazolium lysine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_1513 and upp are separated by 211 nucleotidesupp and Shew_1515 are separated by 94 nucleotides Shew_1513: Shew_1513 - phosphoribosylaminoimidazole synthetase (RefSeq), at 1,735,736 to 1,736,773 _1513 Shew_1514: upp - uracil phosphoribosyltransferase (RefSeq), at 1,736,985 to 1,737,611 upp Shew_1515: Shew_1515 - Na+/H+ antiporter NhaC (RefSeq), at 1,737,706 to 1,739,025 _1515 Position (kb) 1736 1737 1738Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 1736.251 kb on + strand, within Shew_1513at 1737.150 kb on + strand, within uppat 1737.570 kb on + strandat 1737.601 kb on + strandat 1737.699 kb on + strandat 1737.759 kb on + strandat 1737.767 kb on - strandat 1737.899 kb on - strand, within Shew_1515at 1738.563 kb on + strand, within Shew_1515

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium lysine 10 mM
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1,736,251 + Shew_1513 0.50 +5.2
1,737,150 + upp Shew_1514 0.26 -0.2
1,737,570 + -0.2
1,737,601 + -2.1
1,737,699 + -0.3
1,737,759 + -0.6
1,737,767 - -0.3
1,737,899 - Shew_1515 0.15 -1.9
1,738,563 + Shew_1515 0.65 +0.9

Or see this region's nucleotide sequence